Actin beta / ACTB antibody

Principal name

Actin beta / ACTB antibody

Alternative names for Actin beta / ACTB antibody

Actin cytoplasmic 1, Beta-Actin

SwissProt ID

P29751 (Rabit), P60706 (Chick), P60709 (Human), P60710 (Mouse), P60711 (Rat), P60712 (Bovin)

Gene ID

60 (ACTB), 81822 (Actb), 11461

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Can (Canine), Dros (Drosophila), Fe (Feline), GP (Guinea Pig), Por (Porcine), Sh (Sheep), Bov (Bovine), Chk (Chicken), Fi (Fish), Le (Leech), Dictyostelium, Carp, Xen (Xenopus), Mky (Monkey), Ye (Yeast), Ce (C. elegans), All Species, Hst (Hamster), Ze (Zebrafish), Prim (Primate), Eq (Equine), Gt (Goat), Arabidopsis, Insect

Available hosts

Rabbit, Mouse, Chicken, Goat

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F), ChIP assay (ChIP)

Background of Actin beta / ACTB antibody

Actin is a globular protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is the monomeric subunit of two types of filaments: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actin participates in many important cellular processes including muscle contraction, cell sigling, motility and division, cytokinesis, vesicle and organelle movement, and the establishment and maintence of cell junctions and shape. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cells as components of the cytoskeleton, and as mediators of interl cell motility (Doherty GJ, et al 2008). Among these actins, beta actin, which is expressed constantly, and at high levels, in all the tissue types used in protein research except muscle (skeletal and cardiac). Beta actin acts as a useful loading control in Western blot alysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. Loading controls can also be used to compare amounts of protein tested (or loaded) per gel lane in western blotting. While very useful when comparing lysates of the same origin (for example transfected and untransfected HEK 293s), comparing lysates from different tissues can be complex. Actin is almost universally expressed, but different tissues express different amounts. In western blotting, an actin antibody titered to the detection limit can show these differences, while an actin antibody used well above the detection limit can show a response that appears equally robust in all samples probed. Therefore caution must be used when using actin or another 'loading control antibody.'

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Actin beta / ACTB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTB (RC203643, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polyclonal anti-ACTB antibody at 1:500 dilution. Rabbit Purified Can, Hu, Mky, Ms WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

Actin beta / ACTB (Loading Control) antibody

Western blot analysis of β-Actin in Jurkat cell lysate (left lane) and mouse intestine tissue lysate (right lane). Rabbit Purified Hu, Ms, Rb WB
0.1 mg / €390.00
  Acris Antibodies GmbH

Actin beta / ACTB (C-term) (Loading Control) antibody

Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Western Blot: Beta Actin Antibody [TA336689] - Rabbit polyclonal at 1/5000. Rabbit Purified Hu, Ms, Por, Rt C, IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Western Blot: Beta Actin Antibody [TA336690] - Detection of actin in 3T3 (20ug) lysates   ECL detection 30 seconds.     Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Western Blot: Beta Actin Antibody (8H10D10) [TA336770] - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates. Mouse IgG2b 8H10D10 Purified Hu, Ms, Prim, Rt E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Host: Rabbit; Target Name: ACTB; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml.ACTB is strongly supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Eq, Gt, Hu, Ms, Por, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Western blot detection of beta actin in C6,3T3,COS7,CHO-k1,K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted).Predicted band size:45KDa.Observed band size:45KDa. Mouse IgG2b 8F10-G10 Purified Arabidopsis, Hst, Rb WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Beta-actin Monoclonal Antibody (Cat.-No AM11015SU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Mouse 8H10D10 Ascites Hu P, WB

Regular Price: 0.1 ml / €370.00

Special Price: 0.1 ml / €231.00

  Acris Antibodies GmbH

Actin beta / ACTB antibody

Human Prostate, Stroma: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1E7 Purified Hu, Ms, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Actin beta / ACTB antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Actin beta / ACTB antibody

Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml] Mouse IgG1 3G4-F9 Purified Hu, Ms E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Actin beta / ACTB antibody

Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml] Mouse IgG1 1E7 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Actin beta / ACTB antibody

Human Breast; IHC Information: Paraffin embedded breast tissue, tested with an antibody dilution of 5 ug/ml.; ACTB Antibody - middle region (ARP40174_P050) in Human Breast cells using Immunohistochemistry Rabbit Aff - Purified Can, Ce, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Actin beta / ACTB antibody

Immunofluorescence: Beta-actin Antibody (AC-15) [NB600-501] - Immunofluroescence  Cultured FS-11 human fibroblast cell line stained using Monoclonal Anti-â Actin, Clone No. AC-15 (NB600-501). Mouse IgG1 AC-15 Ascites Bov, Can, Chk, Fe, Fi, GP, Hu, Le, Ms, Por, Rb, Rt, Sh C, E, ICC/IF, P, WB
0.1 ml / €400.00
  Novus Biologicals Inc.

Actin beta / ACTB (N-term) antibody

Actin beta / ACTB Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €320.00
  SICGEN

Actin beta / ACTB (N-term) antibody

AM21043PU Actin beta staining of Raji cell lysate by western blotting. Rabbit IgG SP124 Purified Hu F, P, WB
0.5 ml / €510.00
  Acris Antibodies GmbH

Actin beta / ACTB (N-term) antibody

AM21043PU Actin beta staining of Raji cell lysate by western blotting. Rabbit IgG SP124 Purified Hu F, P, WB
1 ml / €730.00
  Acris Antibodies GmbH

Actin beta / ACTB (N-term) antibody

AM21043PU Actin beta staining of Raji cell lysate by western blotting. Rabbit IgG SP124 Purified Hu F, P, WB
0.1 ml / €290.00
  Acris Antibodies GmbH

Actin beta / ACTB (N-term) antibody

Leiomyoma stained with Anti-beta Actin Rabbit Purified Bov, Can, Chk, Fe, GP, Hu, Ms, Por, Rb, Rt, Sh P
0.5 ml / €360.00
  Acris Antibodies GmbH

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