Actin beta / ACTB antibody

Principal name

Actin beta / ACTB antibody

Alternative names for Actin beta / ACTB antibody

Actin cytoplasmic 1, Beta-Actin

SwissProt ID

P29751 (Rabit), P60706 (Chick), P60709 (Human), P60710 (Mouse), P60711 (Rat), P60712 (Bovin)

Gene ID

60 (ACTB), 81822 (Actb), 11461

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Fe (Feline), GP (Guinea Pig), Por (Porcine), Sh (Sheep), Ce (C. elegans), Dros (Drosophila), All Species, Hst (Hamster), Mky (Monkey), Ze (Zebrafish), Prim (Primate), Eq (Equine), Gt (Goat), Arabidopsis, Insect

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), ChIP assay (ChIP)

Background of Actin beta / ACTB antibody

Actin is a globular protein found in all eukaryotic cells (the only known exception being nematode sperm) where it may be present at concentrations of over 100 uM. It is the monomeric subunit of two types of filaments: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. Actin participates in many important cellular processes including muscle contraction, cell sigling, motility and division, cytokinesis, vesicle and organelle movement, and the establishment and maintence of cell junctions and shape. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cells as components of the cytoskeleton, and as mediators of interl cell motility (Doherty GJ, et al 2008). Among these actins, beta actin, which is expressed constantly, and at high levels, in all the tissue types used in protein research except muscle (skeletal and cardiac). Beta actin acts as a useful loading control in Western blot alysis. Loading controls serve a number of purposes; they ensure the protein of interest has been correctly loaded on the gel, that it is being transferred correctly, and that all reagents are functioning normally. Loading controls can also be used to compare amounts of protein tested (or loaded) per gel lane in western blotting. While very useful when comparing lysates of the same origin (for example transfected and untransfected HEK 293s), comparing lysates from different tissues can be complex. Actin is almost universally expressed, but different tissues express different amounts. In western blotting, an actin antibody titered to the detection limit can show these differences, while an actin antibody used well above the detection limit can show a response that appears equally robust in all samples probed. Therefore caution must be used when using actin or another 'loading control antibody.'

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Actin beta / ACTB antibody

  Rabbit polyclonal anti-ACTB(beta Actin) antibody, Loading control  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTB (RC203643, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with Rabbit polyclonal anti-ACTB antibody at 1:500 dilution. Rabbit Purified Can, Hu, Mky, Ms WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

Actin beta / ACTB (Loading Control) antibody

Western blot analysis of β-Actin in Jurkat cell lysate (left lane) and mouse intestine tissue lysate (right lane). Rabbit Purified Hu, Ms, Rb WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Actin beta / ACTB (C-term) (Loading Control) antibody

  Rabbit Polyclonal Beta-actin Antibody  
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Polyclonal beta-Actin Antibody  
Western Blot: Beta Actin Antibody [TA336689] - Rabbit polyclonal at 1/5000. Rabbit Purified Hu, Ms, Por, Rt C, IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Polyclonal beta-Actin Antibody  
Western Blot: Beta Actin Antibody [TA336690] - Detection of actin in 3T3 (20ug) lysates   ECL detection 30 seconds.     Lane 1 - 1:15,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - 1:15,000 dilution Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Mouse Monoclonal beta-Actin Antibody (8H10D10)  
Western Blot: Beta Actin Antibody (8H10D10) [TA336770] - Analysis of Beta Actin expression in 1) HeLa 2) HepG2 and 3) Cos7 whole cell lysates. Mouse IgG2b 8H10D10 Purified Hu, Ms, Prim, Rt E, F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Polyclonal Anti-ACTB Antibody  
Host: Rabbit; Target Name: ACTB; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml.ACTB is strongly supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Eq, Gt, Hu, Ms, Por, Rt, Sh, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Mouse Monoclonal beta Actin Antibody  
Western blot detection of beta actin in C6,3T3,COS7,CHO-k1,K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted).Predicted band size:45KDa.Observed band size:45KDa. Mouse IgG2b 8F10-G10 Purified Arabidopsis, Hst, Rb WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Monoclonal anti-Actin-beta Antibody  
Western blot analysis of Raji cell lystate with anti-actinbeta antibody Rabbit IgG SP124 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Beta-actin Monoclonal Antibody (Cat.-No AM11015SU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Mouse 8H10D10 Ascites Hu P, WB

Regular Price: 0.1 ml / €370.00

Special Price: 0.1 ml / €231.00

  Acris Antibodies GmbH

Actin beta / ACTB antibody

Human Prostate, Stroma: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1E7 Purified Hu, Ms, Rt E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Actin beta / ACTB antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Actin beta / ACTB (N-term) antibody

Actin beta / ACTB Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €320.00
  SICGEN

Actin beta / ACTB antibody

  Rabbit Polyclonal antibody to beta Actin (actin, beta)  
Sample (30 ug of whole cell lysate). A: A431 . B: H1299. 10% SDS PAGE. TA308840 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Mouse monoclonal anti-ACTB(beta Actin) antibody, Loading control  
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-beta-Actin monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Mouse IgG 8H10 Purified Can, Hu, Mky, Ms, Rt WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit polyclonal anti-Actin-pan antibody  
Western blot analysis of extracts from HeLa cells, using Actin-pan antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Polyclonal anti-ß-Actin Antibody  
Western blot analysis of Hela (lane 1), mouse spleen (lane 2) and NIH3T3 (lane 3) cells, using anti-β-actin antibody (poly6221). Rabbit IgG Poly6221 Purified Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Mouse Monoclonal anti-ß-actin Antibody  
NIH/3T3 cell extract was western blotted with 2F1-1. Proteins were visualized using a HRP goat anti-mouse antibody and a chemiluminescence detection system. Mouse IgG2b 2F1-1 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Polyclonal beta-Actin Antibody  
Western Blot: Beta Actin Antibody [TA336477] - analysis using beta actin antibody: Decreasing amounts of human ovary tissue lysate were probed using beta actin antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, and D) 10 ug per lane. Rabbit Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Actin beta / ACTB antibody

  Rabbit Monoclonal Antibody against Beta-Actin  
Actin beta / ACTB Rabbit RM112 Purified ChIP, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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