Actin gamma / ACTG antibody

Principal name

Actin gamma / ACTG antibody

SwissProt ID

P63258 (Bovin), P63259 (Rat), P63260 (Mouse), P63261 (Human), Q5ZMQ2 (Chick)

Gene ID

71 (ACTG1), 60 (ACTB)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Ze (Zebrafish), Chk (Chicken), Por (Porcine), Rb (Rabbit), Xen (Xenopus)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of Actin gamma / ACTG antibody

Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus,  but the sequence of each of these actins is highly conserved in higher vertebrates.
β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.

  1. Quick-Filters:
  2. Highly Validated (1)

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Actin gamma / ACTG antibody

Simple Western: Actin Gamma 1 Antibody [TA336691] - Simple Western lane view shows a specific band detected for Actin Gamma 1 in HeLa lysate.  This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa ( Rabbit Purified Hu, Ms ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin gamma / ACTG antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. B: JC. C: BCL-1. 10% SDS PAGE. TA308842 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin gamma / ACTG antibody

Western blot analysis of human SW-620 cell lysates with anti-ACTG antibody at 1:300. Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin gamma / ACTG antibody

Predicted band size: 42 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin gamma / ACTG antibody

Predicted band size: 42 kDa . Positive control: Human lymphoma tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 second) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Actin gamma / ACTG (Cytopl.) antibody

Methanol fixed human dermal fibroblasts immunostained with 2A3 (1/500) Mouse IgG2b 2A3 Purified Chk, Hu, Ms, Por, Rb, Rt, Ze C, E, F, ICC/IF, P, WB
0.1 mg / €320.00
  Acris Antibodies GmbH

Actin gamma / ACTG antibody

Western blot analysis of ACTG1 antibody (Center) (AP17084PU-N) in HepG2 cell line lysates (35 µg/lane). ACTG1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Actin gamma / ACTG (1-119) antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Mky, Ze P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Actin gamma / ACTG antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with anti-VASP rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 1G6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Actin gamma / ACTG antibody

Western blot analysis of ACTG1 in NIH/3T3 ( 20 µg ) lysates using ACTG1 polyclonal antibody ( Cat # PAB12510 ). Lane 1 - 1 : 5,000 dilution. Lane 2 - 1 : 10,000 dilution. Lane 3 - 1 : 15,000 dilution. Rabbit Hu, Ms IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

Actin gamma / ACTG antibody

Actin gamma / ACTG Rabbit Aff - Purified Hu, Ms ICC/IF, IP, WB
25 µl / €200.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Actin gamma / ACTG

Actin gamma / ACTG Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Actin gamma / ACTG

Actin gamma / ACTG Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ACTG1 overexpression lysate

ACTG1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
  • LinkedIn