ACTL6A / BAF53 antibody

Principal name

ACTL6A / BAF53 antibody

Alternative names for ACTL6A / BAF53 antibody

BAF53A, INO80K, Actin-like protein 6A, 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, INO80 complex subunit K

SwissProt ID

O96019 (Human), Q9Z2N8 (Mouse)

Gene ID

86 (ACTL6A), 56456 (Actl6a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of ACTL6A / BAF53 antibody

ACTL6A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling.
ACTL6A is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix.
ACTL6A is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.

General readings

Harata M, Mochizuki R, Mizuno S.
Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues.
Biosci Biotechnol Biochem. 1999 May;63(5):917-23.
PMID: 10380635

Items 1 to 20 of 22 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACTL6A / BAF53 (C-term) antibody

  Goat Polyclonal Antibody against ACTL6A / ACTL6B  
TA302939 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

  Rabbit polyclonal anti-ACL6A antibody  
Western blot analysis of extracts from Jurkat cells and COLO205 cells, using ACL6A antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

  Rabbit anti-ACTL6A Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ACTL6A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

  Rabbit Polyclonal Anti-ACTL6A Antibody  
WB Suggested Anti-ACTL6A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

  Rabbit Polyclonal Anti-ACTL6A Antibody - middle region  
Host: Rabbit; Target Name: ACTL6A; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells. Mouse IgG2b N336B/83 Supernatant Hu, Ms, Rt ICC/IF, WB
5 ml / €460.00
  Antibodies Incorporated

NeuroMab

ACTL6A / BAF53 antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells. Mouse IgG2b N336B/83 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €540.00
  Antibodies Incorporated

NeuroMab

ACTL6A / BAF53 antibody

Immunofluorescence analysis of HuvEc cells, using ACTL6A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech

ACTL6A / BAF53 antibody

Immunofluorescence analysis of HuvEc cells, using ACTL6A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Actl6a - mouse gene knockout kit via CRISPR

Actl6a - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Actl6a - mouse gene knockout kit via CRISPR

Actl6a - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Actl6a - mouse gene knockout kit via CRISPR

Actl6a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Actl6a - mouse gene knockout kit via CRISPR

Actl6a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ACTL6A / BAF53 (transcript variant 1)

  Recombinant protein of human actin-like 6A (ACTL6A), transcript variant 1  
ACTL6A / BAF53 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ACTL6A overexpression lysate

ACTL6A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ACTL6A overexpression lysate

ACTL6A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ACTL6A overexpression lysate

ACTL6A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ACTL6A overexpression lysate

ACTL6A overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ACTL6A (mGFP-tagged) - Human actin-like 6A (ACTL6A), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, ACTL6A (mGFP-tagged) - Human actin-like 6A (ACTL6A), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, ACTL6A (mGFP-tagged) - Human actin-like 6A (ACTL6A), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, ACTL6A (mGFP-tagged) - Human actin-like 6A (ACTL6A), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Items 1 to 20 of 22 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn