ACTL6A / BAF53 antibody

Principal name

ACTL6A / BAF53 antibody

Alternative names for ACTL6A / BAF53 antibody

BAF53A, INO80K, Actin-like protein 6A, 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, INO80 complex subunit K

SwissProt ID

O96019 (Human), Q9Z2N8 (Mouse)

Gene ID

86 (ACTL6A), 51412 (ACTL6B)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Chicken, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P), PLA/Proximity ligation assay (PLA)

Background of ACTL6A / BAF53 antibody

This protein is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This protein is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.

General readings

Harata M, Mochizuki R, Mizuno S.
Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues.
Biosci Biotechnol Biochem. 1999 May;63(5):917-23.
PMID: 10380635

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACTL6A/ACTL6B antibody

The ACTL6A/ACTL6B polyclonal antibody ( Cat # PAB6615 ) staining ( 1 µg/ml ) of Hela lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Purified Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ACTL6A / BAF53 antibody

Immunohistochemistry-Paraffin: BAF53A Antibody [NB100-61628] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-BAF53A used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, PLA, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ACTL6A / BAF53 (C-term) antibody

TA302939 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

Western blot analysis of extracts from Jurkat cells and COLO205 cells, using ACL6A antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

Western blot analysis of extracts of various cell lines, using ACTL6A antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

WB Suggested Anti-ACTL6A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

Host: Rabbit; Target Name: ACTL6A; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ACTL6A / BAF53 antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells. Mouse IgG2b N336B/83 Supernatant Hu, Ms, Rt ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

ACTL6A / BAF53 antibody

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged BAF53a, BAF53b or Kv2.1 plasmids and probed with N336B/83 (left) or N52A/42 (right) TC supe. Note that BAF53a is ubiquitously expressed in many different cell types, including COS cells. Mouse IgG2b N336B/83 Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

ACTL6A / BAF53 antibody

Immunoprecipitation of ACTL6A transfected lysate using anti-ACTL6A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ACTL6A monoclonal antibody (M10), clone 3C4 (H00000086-M10). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

ACTL6A / BAF53 antibody

Western Blot analysis of ACTL6A expression in transfected 293T cell line by ACTL6A monoclonal antibody (M10), clone 3C4.

Lane 1: ACTL6A transfected lysate(47.5 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 3C4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACTL6A / BAF53 antibody

ACTL6A / BAF53 Mouse IgG1 3E4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ACTL6A / BAF53 antibody

Immunofluorescence analysis of HuvEc cells, using ACTL6A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

ACTL6A / BAF53 antibody

Immunofluorescence analysis of HuvEc cells, using ACTL6A Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

ACTL6A / BAF53 antibody

ACTL6A / BAF53 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ACTL6A / BAF53 antibody

Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ACTL6A / BAF53 antibody

Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ACTL6A / BAF53 antibody

Immunohistochemical analysis of BAF53A staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Por, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ACTL6A / BAF53 antibody

NB 100-1421 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ACTL6A / BAF53 antibody

ACTL6A / BAF53 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

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