Acylphosphatase 1 / ACYP1 antibody

Principal name

Acylphosphatase 1 / ACYP1 antibody

Alternative names for Acylphosphatase 1 / ACYP1 antibody

ACYPE, Acylphosphate phosphohydrolase 1

SwissProt ID

P07311 (Human), P41500 (Bovin), P56376 (Mouse)

Gene ID

97 (ACYP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB)

Background of Acylphosphatase 1 / ACYP1 antibody

ACYP1, also known as erythrocyte acylphosphatase, is a cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two acylphophatase isoenzymes exist: ACYP1 and ACYP2. The two isoenzymes share 60% homology and have the same substrate specificity, although ACYP1 has higher catalytic activity than ACYP2. Recombinant human ACYP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

General readings

Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G.
A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase.
J Biol Chem. 2003 Jan 3;278(1):194-9. Epub 2002 Oct 29.
PMID: 12409302

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Acylphosphatase 1 / ACYP1 antibody

TA303184 (0.01µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Acylphosphatase 1 / ACYP1 antibody

Predicted band size: 11 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acylphosphatase 1 / ACYP1 antibody

Predicted band size: 11 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Acylphosphatase 1 / ACYP1 antibody

WB Suggested Anti-ACYP1 Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Acylphosphatase 1 / ACYP1 (72-85) antibody

Testis: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bt, Can, Elephant, Hst, Hu, Mky, Ms, Por, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Acylphosphatase 1 / ACYP1 antibody

Western Blot analysis of ACYP1 expression in transfected 293T cell line by ACYP1 monoclonal antibody (M01), clone 1B2-3A2.

Lane 1: ACYP1 transfected lysate(11.3 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 1B2-3A2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Acylphosphatase 1 / ACYP1 antibody

ACYP1 polyclonal antibody ( Cat # PAB7186 ) ( 0.01 µg/ml ) staining of human testis lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Acylphosphatase 1 / ACYP1 antibody

Western Blot: ACYP1 Antibody [NB100-57091] - NB100-57091 (0.01ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Acylphosphatase 1 / ACYP1 antibody

Immunohistochemistry-Paraffin: ACYP1 Antibody [NBP1-86314] Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Acylphosphatase 1 / ACYP1 (transcript variant 1)

Acylphosphatase 1 / ACYP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Acylphosphatase 1 / ACYP1 (1-99, His-tag)

Acylphosphatase 1 / ACYP1 Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

Acylphosphatase 1 / ACYP1 (1-99, His-tag)

Acylphosphatase 1 / ACYP1 Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Acylphosphatase 1 / ACYP1

Acylphosphatase 1 / ACYP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Acylphosphatase 1 / ACYP1

Acylphosphatase 1 / ACYP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Acylphosphatase 1 / ACYP1

Acylphosphatase 1 / ACYP1 Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

Acylphosphatase 1 / ACYP1

Acylphosphatase 1 / ACYP1 Human Purified
0.5 mg / €870.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

ACYP1 overexpression lysate

ACYP1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

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