ADAM12 / MLTN antibody

Principal name

ADAM12 / MLTN antibody

Alternative names for ADAM12 / MLTN antibody

Meltrin alpha, Disintegrin and metalloproteinase domain-containing protein 12

SwissProt ID

O43184 (Human), Q61824 (Mouse)

Gene ID

8038 (ADAM12)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), Por (Porcine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of ADAM12 / MLTN antibody

ADAM12 is a member of the ADAM (a disintegrin and metalloprotease-like domain) family. Two forms of ADAM12 have been described: ADAM12S and ADAM12L (short and long forms). The short form is a soluble form lacking the transmembrane and cytoplasmic domains. The short form of ADAM12 was reported to provoke myogenesis, and the lack of cytoplasmic domain suggests different regulation pathways (although both forms can be expressed in the same tissue). Other papers investigated the SH3 ligand domains in the cytoplasmic portion of ADAM12, demonstrating regulation routes for ADAM12 via Src and Src tyrosine kinase. ADAM12 is primarily expressed in muscle and bone (although placenta, osteoblasts, and many tumor cell lines also express ADAM12). ADAM12 supports cell adhesion by acting as a cell attachment molecule and binding integrins through the cysteine-rich domain. A marker of skeletal muscle regeneration, it has been implicated in myoblast differentiation and fusion. ADAM12 contains the canonical HExxHxxxxxH zinc metalloproteinase motif. Full length ADAM12 (909 amino acids) has a predicted mass of 99.5 kDa, but due to glycosylation and cyteine-rich regions, the reduced protein migrates to 114 kDa (unprocessed) and 84 kDa (processed). The soluble form of ADAM12 (738 amino acids, predicted mass of 80.5 kDa) is seen as a 92 kDa zymogen and 68 kDa furin-processed form.

General readings

Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E. Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion. J Biol Chem. 2000 May 5;275(18):13933-9. PMID: 10788519

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ADAM12 / MLTN (N-term) antibody

TA302969 staining (0.1µg/ml) of human heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ADAM12 / MLTN antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human breast and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ADAM12 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAM12 / MLTN (225-239) antibody

TA326752 (0.3µg/ml) staining of Mouse Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ADAM12 / MLTN antibody

WB Suggested Anti-ADAM12 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ADAM12 / MLTN (N-term) antibody

Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ADAM12 / MLTN antibody

ADAM12 / MLTN Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ADAM12 / MLTN antibody

Western Blot analysis of ADAM12 expression in transfected 293T cell line (H00008038-T01) by ADAM12 MaxPab polyclonal antibody.

Lane 1: ADAM12 transfected lysate(81.07 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ADAM12 / MLTN antibody

Western Blot analysis of ADAM12 expression in transfected 293T cell line (H00008038-T02) by ADAM12 MaxPab polyclonal antibody.

Lane 1: ADAM12 transfected lysate(80.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ADAM12 / MLTN antibody

Detection limit for recombinant GST tagged ADAM12 is approximately 0.3ng/ml as a capture antibody. Mouse IgG3 1G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ADAM12 / MLTN antibody

ADAM12 polyclonal antibody ( Cat # PAB6697 ) staining ( 0.1 µg/ml ) of human heart lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ADAM12 / MLTN antibody

Human Placenta; WB Suggested Anti-ADAM12 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Placenta; ADAM12 antibody - N-terminal region (ARP41334_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

ADAM12 / MLTN antibody

Immunohistochemistry: ADAM12 Antibody[NB300-889]- (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat IgG Aff - Purified Hu E, P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ADAM12 / MLTN antibody

Western  Blot:  ADAM12 Antibody [NBP1-33718] – Western blot analysis of ADAM12 on A431 lysates using NBP1-33718 at a dilution of 1:3000. 30ug whole cell lysate loaded on 7.5% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ADAM12 / MLTN antibody

Western Blot: ADAM12 Antibody [NBP1-59142] - This Anti-ADAM12 antibody was used in Western Blot of Placenta tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

ADAM12 / MLTN antibody

Immunohistochemistry-Paraffin: ADAM12 Antibody [NBP1-82791] - Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ADAM12 / MLTN

ADAM12 / MLTN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ADAM12 / MLTN

ADAM12 / MLTN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ADAM12 / MLTN

ADAM12 / MLTN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ADAM12 / MLTN

ADAM12 / MLTN Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Antibody Pairs

Catalog No.    

ADAM12 (Human) Matched Antibody Pair

ADAM12 (Human) Matched Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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