ADAM2 / FTNB antibody

Principal name

ADAM2 / FTNB antibody

Alternative names for ADAM2 / FTNB antibody

PH-30, PH30-beta, Fertilin subunit beta, Disintegrin and metalloproteinase domain-containing protein 2, Cancer/testis antigen 15

SwissProt ID

Q60718 (Mouse), Q63202 (Rat), Q99965 (Human)

Gene ID

2515 (ADAM2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of ADAM2 / FTNB antibody

ADAM-2, also known as PH-30?and Fertilin-? was first described as a sperm-egg fusion protein from guinea pig. A member of the metalloproteinase family containing disintigrin-like domains (ADAMs), the function of ADAM-2 is still poorly understood. Unlike ADAM-1, ADAM-2 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. Like the other ADAMs, ADAM-2 domain structure consists of a signal sequence followed by a propeptide domain, a metalloproteinase domain, a disintegrin domain cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. Three isoforms of ADAM-2 are reported to date, which differ in the beginning of the metalloproteinase domain and cysteine-rich domain. The sequences are coded from intronless genes. The longest ADAM-2 message encodes a protein of 735 amino acids, with a predicted mass of 82.5 kDa and a pI of 5.73. The 716 amino acid form of ADAM-2 has a deletion in the start of the metalloproteinase domain, relative to the longer form, and has a predicted mass of 80.2 kDa and pI of 5.75. The shorter form of 579 amino acids shared the deletion in the MP domain, and also has a deletion in the cystein-rich domain. Localized on the surface of sperm, the ADAM-2 isoforms are thought to form a heterodimer with ADAM-1 (fertilin-a), and facilitate sperm-egg fusion, although there is some controversy about the precise actions the proteins play. Integrin a6? on the egg surface is thought to dock with a QDECD motif in the disintegrin domain of ADAM-2, and there is some speculation that ADAM-1/ADAM-2 heterodimewr initiated ADAM-3 production on the cell surface.

General readings

Eto,K., (2002) J. Biol. Chem. 277 (20), 17804-17810

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ADAM2 / FTNB antibody

Predicted band size: 64 kDa . Positive control: 293T and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lane1: 293T cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAM2 / FTNB antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ADAM2 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAM2 / FTNB antibody

WB Suggested Anti-ADAM2 Antibody Titration: 0.2-1 ug/ml; Positive Control: 721_B cell lysate Rabbit Aff - Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

ADAM2 / FTNB antibody

ADAM2 / FTNB Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ADAM2 / FTNB antibody

ADAM2 / FTNB Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ADAM2 / FTNB antibody

Detection limit for recombinant GST tagged ADAM2 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2a 4A2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ADAM2 / FTNB antibody

ADAM2 / FTNB Mouse IgG2a 1B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ADAM2 / FTNB antibody

Human 721_B; WB Suggested Anti-ADAM2 Antibody Titration: 0.2-1 ug/ml. Positive Control: 721_B cell lysate; ADAM2 antibody - middle region (ARP53589_P050) in Human 721_B cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ADAM2 / FTNB antibody

ADAM2 / FTNB Rabbit IgG Aff - Purified Hu WB
0.1 mg / €290.00
  Boster Immunoleader

ADAM2 / FTNB antibody

Western Blot: ADAM2 Antibody [NBP1-59224] - This Anti-ADAM2 antibody was used in Western Blot of 721_B tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

ADAM2 / FTNB antibody

Immunohistochemistry-Paraffin: ADAM2 Antibody [NBP1-85415] - Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in mature spermatogonia. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

ADAM2 / FTNB antibody

Immunohistochemistry-Paraffin: ADAM2 Antibody [NBP1-85416] Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ADAM2 / FTNB

ADAM2 / FTNB Human
2 µg / €300.00
  Abnova Taiwan Corp.

ADAM2 / FTNB

ADAM2 / FTNB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ADAM2 / FTNB

ADAM2 / FTNB Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ADAM2 293T Cell Transient Overexpression Lysate(Denatured)

ADAM2 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

ADAM2 overexpression lysate

ADAM2 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

17 Item(s)

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