ADAMTS2 antibody

Principal name

ADAMTS2 antibody

Alternative names for ADAMTS2 antibody

ADAMTS-2, ADAM-TS2, PCINP, PCPNI, A disintegrin and metalloproteinase with thrombospondin motifs 2, Procollagen I/II amino propeptide-processing enzyme, Procollagen I N-proteinase, Procollagen N-endopeptidase

SwissProt ID

O95450 (Human), P79331 (Bovin), Q8C9W3 (Mouse)

Gene ID

9509 (ADAMTS2)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine)

Available hosts

Rabbit, Mouse

Available applications

Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E)

Background of ADAMTS2 antibody

ADAMTS2 is a member of the larger family of ADAMs (A Disintegrin And Metalloproteinase) metalloproteinases containing thrombospondin (TS) repeats. ADAMTS2 (A Disintegrin And Metalloproteinase with ThromboSpondin-2 motif), also known as Procollagen I N-Proteinase (PNP), was first described in calf skin as a proteinase that processes the amino end of Type-I collagen. PNP expression was found in skin, aorta, liver, tendon, bladder, retina, and skeletal muscle. Later, PNP was found to be a member of a larger family of ADAMs metalloproteinases containing thrombospondin (TS) repeats. Full length human ADAMTS2 contains 1211 amino acids (bovine, 1205 amino acids) and has a predicted mass of 134.7 kDa, but glycosylation and the abundance of cysteine residues gives ADAMTS2 a greater apparent molecular weight on reduced SDS-PAGE gels. Purified ADAMTS2 resolves at a lower molecular weight of 107 kDa, due to cleavage at the furin site. ADAMTS2 contains the canonical HexxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active, cleaving procollagen. In addition to the metalloprotease domain, ADAMTS2 has a propeptide domain, a prohormone convertase (PC, furin) cleavage site, a cysteine-rich domain, and three thrombospondin 1 like domains, followed by a unique C-terminal domain. ADAMTS2 does not have a transmembrane domain, unlike many of the ADAMs proteases, and is a secreted protein, much of which binds to the ECM (extracellular matrix). ADAMTS2 knockout mice develop fragile skin (similar to dermatospaxis), and male infertility. Mutations of the ADAMTS2 gene are responsible for human Ehlers-Danlos syndrome type VII C and bovine dermatosparaxis. ADAMTS2 is involved in collagen biosynthesis and may also play role in development and angiogenesis.

General readings

Li SW et al. Transgenic mice with inactive alleles for procollagen N-proteinase (ADAMTS-2) develop fragile skin and male sterility. Biochem J 355:271-8 (2001). PubMed PMID: 11284712
Tang BL ADAMTS: a novel family of extracellular matrix proteases. Int J Biochem Cell Biol 33:33-44 (2001). PubMed PMID: 11167130
Colige A et al. cDNA cloning and expression of bovine procollagen I N-proteinase: a new member of the superfamily of zinc-metalloproteinases with binding sites for cells and other matrix components. Proc Natl Acad Sci U S A 94:2374-9 (1997). PubMed PMID: 9122202
Colige A et al. Characterization and partial amino acid sequencing of a 107-kDa procollagen I N-proteinase purified by affinity chromatography on immobilized type XIV collagen. J Biol Chem 270:16724-30 (1995). PubMed PMID: 7622483

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ADAMTS2 antibody

Western blot analysis of human fetal brain lysates with anti-ADAMTS2 antibody (1:1000). This antibody identified zymogen form of ADAMTS2 at 130-140kDa, activated forms at 94-105kDa, and breakdown products at 50kDa and 34kDa in human fetal brain lysates. Rabbit Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAMTS2 antibody

Western blot analysis of extracts from K562/HeLa/COLO205/HepG2 cells, using ADAMTS2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAMTS2 antibody

Predicted cell location: Secreted,Extracellular space,Extracellular matrix. Positive control: Human colon and gastric cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using ADAMTS2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAMTS2 antibody

ADAMTS2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

ADAMTS2 antibody

ADAMTS2 monoclonal antibody (M03), clone 7G3 Western Blot analysis of ADAMTS2 expression in PC-12 ( Cat # L012V1 ). Mouse IgG2a 7G3 Purified Hu, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ADAMTS2 antibody

ADAMTS2 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

ADAMTS2 antibody

Western blot analysis of ADAMTS2 expression in A431 (A); MCF7 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ADAMTS2 antibody

Western blot analysis of ADAMTS2 expression in A431 (A); MCF7 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ADAMTS2 antibody

Western blot analysis of ADAMTS2 expression in A431 (A); MCF7 (B); SP2/0 (C); H9C2 (D) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ADAMTS2 antibody

ADAMTS2 Rabbit Aff - Purified Hu P, WB
50 µg / €420.00
  Novus Biologicals Inc.

ADAMTS2 antibody

Immunohistochemistry-Paraffin: ADAMTS2 Antibody [NBP1-89246] Immunohistochemical staining of human cerebellum shows strong positivity in purkinje cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ADAMTS2

ADAMTS2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ADAMTS2

ADAMTS2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

13 Item(s)

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