ADAP1 / CENTA1 antibody

Principal name

ADAP1 / CENTA1 antibody

Alternative names for ADAP1 / CENTA1 antibody

Centaurin alpha 1, Centaurin-alpha-1, PM25, Arf-GAP with dual PH domain-containing protein 1, Putative MAPK-activating protein PM25

SwissProt ID

E9PY16, O75689 (Human)

Gene ID

11033 (ADAP1), 231821 (Adap1)

Available reactivities

Hu (Human), Por (Porcine), Ms (Mouse), Can (Canine), Rt (Rat), Bov (Bovine), GP (Guinea Pig)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot)

Background of ADAP1 / CENTA1 antibody

ADP-ribosylation factor (ARF) family of small GTP-binding proteins are involved in vesicular transport regulation and in controlling cytoskeletal organization and cell adhesion. These proteins are best characterized as regulators of membrane traffic. The Centaurin GTPase-activating protein family comprise a subset of ARF regulatory molecules that transduce PI 3-kinase activation into coordinated control of Arf-dependent pathways. This family includes ASAP1, ACAP1, ACAP2, AGAP1, ARAP1, ARAP2, Centaurin a1 and Centaurin g3. The Centaurin a1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), Centaurin a1 is able to switch off ARF6 and inhibit its ability to mediate b2-adrenoceptor internalization and negatively regulate ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.

General readings

Venkateswarlu K, Oatey PB, Tavare JM, Jackson TR, Cullen PJ.
Identification of centaurin-alpha1 as a potential in vivo phosphatidylinositol 3,4,5-trisphosphate-binding protein that is functionally homologous to the yeast ADP-ribosylation factor (ARF) GTPase-activating protein, Gcs1.
Biochem J. 1999 Jun 1;340 ( Pt 2):359-63.
PMID: 10333475

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ADAP1 / CENTA1 (C-term) antibody

TA302895 (1µg/ml) staining of Human Brain lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ADAP1 / CENTA1 antibody

Sample(30 ug of whole cell lysate). A: A431 . B: HeLaS3. 7.5% SDS PAGE. TA308995 diluted at 1:500 Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAP1 / CENTA1 antibody

Predicted band size: 43 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 15 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAP1 / CENTA1 antibody

Predicted band size: 43 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/162.5 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADAP1 / CENTA1 antibody

WB Suggested Anti-Centa1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Mouse Kidney Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ADAP1 / CENTA1 antibody

Immunofluorescence of purified MaxPab antibody to CENTA1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

ADAP1 / CENTA1 antibody

ADAP1 / CENTA1 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ADAP1 / CENTA1 antibody

ADAP1 polyclonal antibody ( Cat # PAB6491 ) staining ( 1 µg/ml ) of human brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Ms E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ADAP1 / CENTA1 antibody

Western Blot: CENTA1 Antibody [NB300-907] - NB300-907 (1ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ADAP1 / CENTA1 antibody

Western Blot: Centaurin alpha 1 Antibody [NBP1-31179] - Western blot analysis of Centaurin alpha 1 Antibody on: A:293T B:H1299 7.5% SDS PAGE diluted at 1:500 Rabbit IgG Hu Dot, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

ADAP1 / CENTA1 antibody

Immunohistochemistry-Paraffin: Centaurin alpha 1 Antibody [NBP1-88097] - Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal and glial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ADAP1 / CENTA1

ADAP1 / CENTA1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ADAP1 / CENTA1

ADAP1 / CENTA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ADAP1 / CENTA1

ADAP1 / CENTA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ADAP1 overexpression lysate

ADAP1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

CENTA1 293T Cell Transient Overexpression Lysate(Denatured)

CENTA1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

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