Adenosine kinase antibody

Principal name

Adenosine kinase antibody

Alternative names for Adenosine kinase antibody

ADK, AK, Adenosine 5'-phosphotransferase

SwissProt ID

P55263 (Human), P55264 (Mouse), Q64640 (Rat)

Gene ID

132 (ADK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of Adenosine kinase antibody

Adenosine kinase (ATP:adenosine 5-prime-phosphotransferase) is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a potentially important regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of ADK could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as antiinflammatory agents. The encoded protein does not present any sequence similarities to other well-characterized mammalian nucleoside kinases. In contrast, 2 regions were identified with significant sequence identity to microbial ribokinase and fructokinases and a bacterial inosine/guanosine kinase. Thus, ADK is a structurally distinct mammalian nucleoside kinase that appears to be akin to sugar kinases of microbial origin. Animal studies have demonstrated that a deficiency of adenosine metabolism a powerful contributor to the development of neonatal hepatic steatosis, providing a model for the rapid development of postnatally lethal fatty liver.

General readings

1. Mathews II , et al. (1998). Biochemistry 10;37(45):15607-20.
2. Lindberg B, Klenow H, Hansen K (1967). J. Biol. Chem. 242 (3): 350-6.
3. CAPUTTO R (1951). J. Biol. Chem. 189 (2): 801-14.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Adenosine kinase antibody

Western blot analysis of extracts from RAW264.7 cells, using ADK antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adenosine kinase (22-362) antibody

Western blot analysis: The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT4F8 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Adenosine kinase (22-362) antibody

Western blot analysis: The cell lysates (35 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADK (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT4F8 Purified Hu E, F, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

Adenosine kinase (N-term) antibody

The anti-ADK Pab is used in Western blot to detect ADK in mouse bladder tissue lysate Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Adenosine kinase (C-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ADK antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Adenosine kinase antibody

Western blot (WB) analysis of ADK antibody (Cat.-No.: AP20391) in extracts from RAW264.7 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Adenosine kinase antibody

ADK polyclonal antibody (A01), Lot # 051116JC01 Western Blot analysis of ADK expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Adenosine kinase antibody

Detection limit for recombinant GST tagged ADK is approximately 10ng/ml as a capture antibody. Mouse IgG2a 4E7 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Adenosine kinase antibody

The ADK polyclonal antibody ( Cat # PAB4653 ) is used in Western blot to detect ADK in mouse bladder tissue lysate . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Adenosine kinase antibody

Western blot analysis of ADK polyclonal antibody ( Cat # PAB4654 ) in mouse liver tissue lysate ( 35 µg/lane ) . ADK ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Adenosine kinase antibody

Western blot analysis of extracts from RAW264.7/NIH-3T3 cells, using ADK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Adenosine kinase antibody

Western blot analysis of extracts from RAW264.7/NIH-3T3 cells, using ADK Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Adenosine kinase antibody

Immunofluorescent analysis of Adenosine Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Adenosine kinase antibody

Immunofluorescent analysis of Adenosine Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Adenosine kinase antibody

Immunofluorescent analysis of Adenosine Kinase staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Adenosine kinase antibody

Western Blot: ADK Antibody [NBP1-19546] - extracts from RAW264.7 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Adenosine kinase antibody

Adenosine kinase Rabbit IgG Hu WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Adenosine kinase antibody

Immunohistochemistry-Paraffin: Adenosine Kinase Antibody [NBP1-90197] - Immunohistochemical staining of human lymph node shows strong nuclear positivity in cells outside the reaction centra. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Adenosine kinase (transcript variant ADK-short)

Adenosine kinase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Adenosine kinase (22-362, His-tag)

Adenosine kinase Human Purified > 95 % by SDS-PAGE E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

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