Adenylate kinase 3 / AK3 antibody

Principal name

Adenylate kinase 3 / AK3 antibody

Alternative names for Adenylate kinase 3 / AK3 antibody

AK3L1, AK6, AKL3L, GTP:AMP phosphotransferase mitochondrial

SwissProt ID

P08760 (Bovin), P29411 (Rat), Q9UIJ7 (Human), Q9WTP7 (Mouse)

Gene ID

50808 (AK3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Por (Porcine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Adenylate kinase 3 / AK3 antibody

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Noda, L.H. et al. (1973) The Enzymes. Vol. 8, 279-305
Khoo, J.C. et al. (1972) Biochim. Biophys. Acta 268, 98-101
Tomasselli, A.G. et al. (1979) Eur.J.Biochemolecules 93, 257-262
Lee, Y. et al. (1998) J. Biochem. 123, 47-54.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Adenylate kinase 3 / AK3 antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AK3 mouse monoclonal antibody. (TA501819) Mouse IgG1 OTI5A1 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK3 mouse monoclonal antibody. (TA501834) Mouse IgG1 OTI3G1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AK3 mouse monoclonal antibody. (TA501835) Mouse IgG2a OTI6B7 Purified Can, Hu, Mky, Rt P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AK3 mouse monoclonal antibody. (TA501888) Mouse IgG2b OTI4C4 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

Western Blot analysis of AK3 expression in transfected 293T cell line (H00050808-T01) by AK3 MaxPab polyclonal antibody.

Lane 1: AK3 transfected lysate(24.97 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu, Ms E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

Western Blot analysis of AK3 expression in transfected 293T cell line (H00050808-T02) by AK3 MaxPab polyclonal antibody.

Lane 1: AK3 transfected lysate(24.97 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu, Ms E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

WB Suggested Anti-AK3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Liver Rabbit IgG Purified Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Adenylate kinase 3 / AK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (RC204408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3. Mouse IgG1 OTI6D6 Purified Can, Hu, Mky, Rt WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (RC204408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3. Mouse IgG1 OTI4A2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501863) Mouse IgG1 OTI6G4 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adenylate kinase 3 / AK3 antibody

Immunohistochemistry: Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromogen. Mouse IgG1 SJB3-36 Purified Hu, Ms E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Adenylate kinase 3 / AK3 antibody

Immunohistochemistry: Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromogen. Mouse IgG1 SJB3-36 Purified Hu, Ms E, P, WB

Regular Price: 50 µl / €235.00

Special Price: 50 µl / €165.00

  Acris Antibodies GmbH

Adenylate kinase 3 / AK3 antibody

AK3L1 MaxPab polyclonal antibody. Western Blot analysis of AK3L1 expression in human liver. Mouse Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

Adenylate kinase 3 / AK3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

AK3 MaxPab rabbit polyclonal antibody. Western Blot analysis of AK3 expression in HeLa. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

Western Blot analysis of AK3 expression in transfected 293T cell line (H00050808-T01) by AK3 MaxPab polyclonal antibody.

Lane 1: AK3 transfected lysate(25.60 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

AK3 monoclonal antibody (M01), clone 1D2 Western Blot analysis of AK3 expression in HepG2 ( Cat # L019V1 ). Mouse IgG1 1D2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

Western blot analysis of recombinant human AK isozymes. Lane 1 : AK1. Lane 2 : AK2. Lane 3 : AK3. Lane 4 : mouse liver. To probed with AK3 monoclonal antibody , clone SJB3 - 36 ( 1 : 1000 ) ( Cat # MAB1065 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 SJB3-36 Hu, Ms C, E, WB
0.1 ml / €400.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

Western blot analysis of AK3L1 polyclonal antibody ( Cat # PAB3989 ) in mouse kidney tissue lysate ( 35 µg/lane ) . AK3L1 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 50dilution ) . Rabbit IgG Purified Hu E
0.4 ml / €390.00
  Abnova Taiwan Corp.

Adenylate kinase 3 / AK3 antibody

Human Liver; WB Suggested Anti-AK3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; AK3 antibody - N-terminal region (ARP56872_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

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