Adiponectin antibody

Principal name

Adiponectin antibody

Alternative names for Adiponectin antibody

ADIPOQ, ACDC, ACRP30, APM1, GBP28, Adipocyte, C1q and collagen domain-containing protein, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein

SwissProt ID

Q15848 (Human), Q3Y5Z3 (Bovin), Q60994 (Mouse), Q8K3R4 (Rat)

Gene ID

11450 (Adipoq), 9370 (ADIPOQ), 246253

Available reactivities

Ms (Mouse), Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Rat, Chicken

Available applications

Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Frozen Sections (C), Radioimmunoassay (R), Flow Cytometry (F)

Background of Adiponectin antibody

Adepnectin (Adpn) is a protein hormone, exclusively secreted from adipose tissue in to the blood stream that modulates number of metabolic processes, including glucose regulation and fatty acid catabolism. Plasma levels of Adpn are relatively high compared to many other hormones and the concentrations are inversely correlated with body fat percentage in adults, while the association in infants and young children is mofre unclear. Adiponectin is secreted into the bloodsteam where it accounts for approximately 0.01% of all plasma protein at around 5-10 μg/mL. Plasma concentrations reveal a sexual dimorphism, with females having higher levels than males. Levels of adiponectin are reduced in diabetics compared to non-diabetics. Weight reduction significantly increases circulating levels. The hormone plays a role in the suppression of the metabolic derangements that may result in type 2 diabetes, obesity, atherosclerosis and non-alcoholic fatty liver disease (NAFLD). Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin, but the two hormones perform complementary actions. Adiponectin was first characterized in mice as a transcript overexpressed in preadipocytes (precursors of fat cells) differentiating into adipocytes. The human homologue was identified as the most abundant transcript in adipose tissue.
Adiponectin is a 244-amino-acid-long polypeptide, Adpn gene is located on chromosome 3p27a region high lighted as affecting genetic susceptibility to type 2 diabetes. The globular structure of adpn resembles the TNF structure despite unrelated prortein sequences. Primarily the Adpn has three distinct structural motifs, first is a short signal squence that targets the hormone for secretion outside the cell; next is a short variable region thatis species related and the third is a 65-amino acid region with similarity to collagenous proteins; the last is a globular domain. Overall this gene shows similarity to the complement 1Q factors. Adpn exist as self associated homotrimers containing three molecules of Adpn, these homotrimers continue to associate with other Adpn molecules to form hexamers ans dodecamers. The role of these high molecular aggragates remain to be elucidated. Adiponectin binds to a two GPCR that have been identified and characterized. hese have distinct tissue specificities within the body and have different affinities to the various forms of adiponectin. The receptors affect the downstream target AMP kinase, an important cellular metabolic rate control point. Expression of the receptors are correlated with insulin levels, as well as reduced in mouse models of diabetes, particularly in skeletal muscle and adipose tissue.

General readings

Joachim Fruebis, et al(2001) PNAS 98(4) 2005-2010
Das,K., et al(2001) Biochem. Biophys.Res.Commun. 280(4)1120-1129
Yamauchi T., et al(2001) Nature Medicine 7(8) 941-946
Berg AH, et al(2001) Nature Medicine 7(8) 947-952
Yamauchi. T., et al(2002) Nature Medicine 8(11) 1288-1295

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503801) Mouse IgG1 OTI1B2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ADIPOQ mouse monoclonal antibody. (UM500072; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB104 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ADIPOQ mouse monoclonal antibody. (UM500072; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB104 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Adiponectin antibody

Western Blot: Adiponectin Antibody [TA336486] - Western blot analysis of Adoponectin in 1. human liver 2. rat liver 3. HL60 Rabbit Purified Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503826) Mouse IgG1 OTI1H1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Anti-ADIPOQ mouse monoclonal antibody (TA503913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADIPOQ(RC215161). Mouse IgG2b OTI7B11 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804768) Mouse IgG2b OTI3G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin (C-term) antibody

Western blot analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Adiponectin (N-term) antibody

Western blot analysis of adiponectin in rat brain cell lysate with adiponectin antibody at (A) 1, (B) 2, and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

Predicted band size: 26 kDa . Positive control: Human liver cancer and mouse skeletal muscle tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane2: Mouse skeletal muscle tissue lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

Predicted band size: 26 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

Recombinant human adiponectin (1 μg/lane) was resolved by SDS-PAGE electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-adiponectin antibody. The protein was visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADIPOQ (RC229756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPOQ. Mouse IgG2a OTI1A8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804438) Mouse IgG2a OTI7H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804554) Mouse IgG1 OTI3F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804555) Mouse IgG1 OTI2C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804576) Mouse IgG1 OTI3E12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804590) Mouse IgG1 OTI1G4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ADIPOQ Mouse monoclonal antibody. (TA804591; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI3D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADIPOQ (RC229756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPOQ. Mouse IgG1 OTI7G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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