Adiponectin antibody

Principal name

Adiponectin antibody

Alternative names for Adiponectin antibody

ADIPOQ, ACDC, ACRP30, APM1, GBP28, Adipocyte, C1q and collagen domain-containing protein, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein

SwissProt ID

Q15848 (Human), Q3Y5Z3 (Bovin), Q60994 (Mouse), Q8K3R4 (Rat)

Gene ID

9370 (ADIPOQ), 11450 (Adipoq), 246253

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine), Rb (Rabbit)

Available hosts

Mouse, Rat, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), ELISA (capture) (E(capture)), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Radioimmunoassay (R), Flow Cytometry (F)

Background of Adiponectin antibody

Adiponectin, alternately named Adipocyte complement-related protein of 30 kDa (Acrp30), adipoQ, adipose most abundant gene transcript 1 (apM1), and gelatin-binding protein of 28 kDa (GBP28), is an adipocyte-specific, secreted protein with potential roles in glucose and lipid homeostasis. Circulating Adiponectin levels are high, accounting for approximately 0.01% of total plasma protein . Adiponectin contains a modular structure that includes an N-terminal collagen-like domain followed by a C-terminal globular domain with significant sequence and structural resemblance to the complement factor C1q . Although they share little sequence identity, similar three-dimensional structure and certain conserved amino acid residues suggest an evolutionary link between the C1q-like domain of Adiponectin and members of the TNF superfamily . Adiponectin assembles into different complexes including trimers (low molecular weight), hexamers (middle molecular weight), and higher order oligomeric structures (high molecular weight) that may affect biological activity . Adiponectin is induced during adipocyte differentiation and its secretion is stimulated by insulin . Two receptors for Adiponectin, termed AdipoR1 and AdipoR2, have been cloned . Although functionally distinct from G-protein-coupled receptors, the genes encode predicted proteins containing 7 transmembrane domains. AdipoR1 is highly expressed in skeletal muscle, while AdipoR2 is primarily found in hepatic tissues.

Injection of Adiponectin into non-obese diabetic mice leads to an insulin-independent decrease in glucose levels . This is likely due to insulin-sensitizing effects involving Adiponectin regulation of triglyceride metabolism . A truncated form of Adiponectin (gAdiponectin) containing only the C-terminal globular domain has been identified in the blood, and recombinant gAdiponectin has been shown to regulate weight reduction as well as free fatty acid oxidation in mouse muscle and liver . The full-length recombinant Adiponectin protein is apparently less potent at mediating these effects . The mechanism underlying the role of Adiponectin in lipid oxidation may involve the regulation of expression or activity of proteins associated with triglyceride metabolism including CD36, acyl CoA oxidase, AMPK, and PPAR. .

Although Adiponectin-regulation of glucose and lipid metabolism in humans is less clear, similar mechanisms may also be in place . A negative correlation between obesity and circulating Adiponectin has been well established , and Adiponectin levels increase concomitantly with weight loss . Decreased Adiponectin levels are associated with insulin resistance and hyperinsulinemia, and patients with type-2 diabetes are reported to exhibit decreased circulating Adiponectin . Thiazolidinediones, a class of insulin-sensitizing, anti-diabetic drugs, elevate Adiponectin in insulin-resistant patients . In addition, high Adiponectin levels are associated with a reduced risk of type-2 diabetes . Using magnetic resonance spectroscopy it has been demonstrated that intracellular lipid content in human muscle negatively correlates with Adiponectin levels, potentially due to Adiponectin-induced fatty acid oxidation .

Adiponectin may also play anti-atherogenic and anti-inflammatory roles. Adiponectin plasma levels are decreased in patients with coronary artery disease . Furthermore, neointimal thickening of damaged arteries is exacerbated in Adiponectin-deficient mice and is inhibited by exogenous Adiponectin . Adiponectin inhibits endothelial cell expression of adhesion molecules in vitro, suppressing the attachment of monocytes . In addition, Adiponectin negatively regulates myelomonocytic progenitor cell growth and TNF-. production in macrophages .

General readings

Scherer, P.E. et al. (1995) J. Biol. Chem. 270:26746.

Fruebis, J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2005.

Berg, A.H. et al. (2002) Trends Endocrinol. Metab. 13:84.

Arita, Y. et al. (1999) Biochem. Biophys. Res. Commun. 257:79.

Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.

Kishore, U. and K.B. Reid (2000) Immunopharmacology 49:159.

Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.

Hu, E. et al. (1996) J. Biol. Chem. 271:10697.

Yamauchi, T. et al. (2003) Nature 423:762.

Berg, A.H. et al. (2001) Nat. Med. 7:947.

Yamauchi, T. et al. (2001) Nat. Med. 7:941.

Tomas, E. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16309.

Yamauchi, T. et al. (2002) Nat. Med. 8:1288.

Thamer, C. et al. (2002) Horm. Metab. Res. 34:646.

Stefan, N. et al. (2002) J. Clin. Endocrinol. Metab. 87:4652.

Matsubara, M. et al. (2002) Eur. J. Endocrinol. 147:173.

Faraj, M. et al. (2003) J. Clin. Endorinol. Metab. 88:1594.

Weyer, C. et al. (2001) J. Clin. Endocrinol. Metab. 86:1930.

Hotta, K. et al. (2000) Arterioscler. Thromb. Vasc. Biol. 20:1595.

Maeda, N. et al. (2001) Diabetes 50:2094.

Spranger, J. et al. (2003) Lancet 361:1060.

Matsuda, M. et al. (2002) J. Biol. Chem. 277:37487.

Ouchi, N. et al. (1999) Circulation 100:2473.

Yokota, T. et al. (2000) Blood 96:1723.

Ouchi, N. et al. (2001) Circulation 103:1057.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Adiponectin antibody

  ADIPOQ (Adiponectin) mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503801) Mouse IgG1 OTI1B2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody,clone UMAB104  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ADIPOQ mouse monoclonal antibody. (UM500072; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB104 Purified Hu P
0.1 ml / €468.00
  OriGene Technologies, Inc.

UltraMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody,clone UMAB104  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ADIPOQ mouse monoclonal antibody. (UM500072; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min) Mouse IgG1 UMAB104 Purified Hu P
30 µl / €140.00
  OriGene Technologies, Inc.

UltraMAB

Adiponectin antibody

  Rabbit Polyclonal Adiponectin/Acrp30 Antibody  
Western Blot: Adiponectin Antibody [TA336486] - Western blot analysis of Adoponectin in 1. human liver 2. rat liver 3. HL60 Rabbit Purified Hu, Ms, Rt C, F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

  ADIPOQ (Adiponectin) mouse monoclonal antibody, clone OTI1H1 (formerly 1H1)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503826) Mouse IgG1 OTI1H1 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ (Adiponectin) mouse monoclonal antibody, clone OTI7B11 (formerly 7B11)  
Anti-ADIPOQ mouse monoclonal antibody (TA503913) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADIPOQ(RC215161). Mouse IgG2b OTI7B11 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI3G10 (formerly 3G10)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804768) Mouse IgG2b OTI3G10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin (C-term) antibody

  Rabbit Polyclonal Adiponectin Antibody  
Western blot analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Adiponectin (N-term) antibody

  Rabbit Polyclonal Adiponectin Antibody  
Western blot analysis of adiponectin in rat brain cell lysate with adiponectin antibody at (A) 1, (B) 2, and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

  Anti-ADIPOQ Rabbit Polyclonal Antibody  
Predicted band size: 26 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

  Rabbit Polyclonal anti-Adiponectin Antibody  
Recombinant human adiponectin (1 μg/lane) was resolved by SDS-PAGE electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-adiponectin antibody. The protein was visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Aff - Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI1A8 (formerly 1A8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADIPOQ (RC229756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPOQ. Mouse IgG2a OTI1A8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI7H8 (formerly 7H8)  
Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804438) Mouse IgG2a OTI7H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI3F12 (formerly 3F12)  
Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804554) Mouse IgG1 OTI3F12 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI2C9 (formerly 2C9)  
Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804555) Mouse IgG1 OTI2C9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI3E12 (formerly 3E12)  
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804576) Mouse IgG1 OTI3E12 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)  
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA804590) Mouse IgG1 OTI1G4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI3D11 (formerly 3D11)  
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ADIPOQ Mouse monoclonal antibody. (TA804591; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI3D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI7G1 (formerly 7G1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADIPOQ (RC229756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPOQ. Mouse IgG1 OTI7G1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Adiponectin antibody

  ADIPOQ mouse monoclonal antibody, clone OTI1F11 (formerly 1F11)  
Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-ADIPOQ Mouse monoclonal antibody. (TA804596; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min) Mouse IgG1 OTI1F11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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