Adipophilin / ADFP antibody

Principal name

Adipophilin / ADFP antibody

Alternative names for Adipophilin / ADFP antibody

Adipose differentiation-related protein, ADRP

SwissProt ID

P43883 (Mouse), Q4PLW0 (Pig), Q99541 (Human), Q9TUM6 (Bovin)

Gene ID

123 (PLIN2)

Available reactivities

Can (Canine), Hu (Human), Rt (Rat), Bov (Bovine), Ms (Mouse), Mky (Monkey), Por (Porcine)

Available hosts

Mouse, Guinea Pig, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E)

Selected product citations ‐ of BM5051

1. Enjoji M, Kohjima M, Ohtsu K, Matsunaga K, Murata Y, Nakamuta M, et al. Intracellular mechanisms underlying lipid accumulation (white opaque substance) in gastric epithelial neoplasms: A pilot study of expression profiles of lipid-metabolism-associated genes. J Gastroenterol Hepatol. 2015 Oct 29. doi: 10.1111/jgh.13216. PubMed PMID: 26513060.
2. Gushima R, Yao T, Kurisaki-Arakawa A, Hara K, Hayashi T, Fukumura Y, et al. Expression of adipophilin in gastric epithelial neoplasia is associated with intestinal differentiation and discriminates between adenoma and adenocarcinoma. Virchows Arch. 2016 Feb;468(2):169-77. doi: 10.1007/s00428-015-1870-0. Epub 2015 Nov 3. PubMed PMID: 26531096.
3. Hisabe T, Yao K, Imamura K, Ishihara H, Yamasaki K, Yasaka T, et al. Novel Endoscopic Findings as Visualized by Magnifying Endoscopy with Narrow-Band Imaging: White Opaque Substance Is Present in Colorectal Hyperplastic Polyps. Digestion. 2016;93(2):127-31. doi: 10.1159/000441841. Epub 2015 Dec 4. PubMed PMID: 26636961.
4. Králová A, Králová Lesná I, Froněk J, Čejková S, Sekerková A, Janoušek L, et al. Macrophage phenotypes in the adipose tissue of postmenopausal women. Physiol Res. 2015;64 Suppl 3:S427-33. PubMed PMID: 26680677. 
5. Imamura, K; Yao, K; Hisabe, T; Nambu, M; Ohtsu, K; Ueo, T; Yano, S; Ishihara, H; Nagahama, T; Kanemitsu, T, Yamasaki, K;Matsui, T;Tanabe, H;Iwashita, A;Daa, T;Yokoyama, S;Matsunaga, K;Enjoji, M. The nature of the white opaque substance within colorectal neoplastic epithelium as visualized by magnifying endoscopy with narrow-band imaging. Endosc Int Open 2016; 04(11): E1151-E1157. DOI: 10.1055/s-0042-116487.

Background of Adipophilin / ADFP antibody

Milk lipid globules from humans, cows and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase. ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells. In tissues, however, expression of adipophilin is restricted to specific cell types, such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells of the male reproductive system, and steatosis or fatty change hepatocytes in alcoholic liver cirrhosis. ADFP may be a possible new marker for the identification of specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.

General readings

1. Heid HW, Moll R, Schwetlick I, Rackwitz HR and Keenan TW. Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res 294, 309-321 (1998)
2. Heid HW, Schnölzer M and TW Keenan. Adipocyte differentiation-related protein is secreted into milk as a constituent of milk lipid globule membrane. Biochem J 320, 1025-1030 (1996)
3. Mrozinzki J, SchwetlicK I, Heid HW, Winter-Simanowski S, Holzbach A, Hinze R, Franke WW and Moll R. Adipophilin and perilipin, two lipid droplet-associated proteins, in the pathohistological differential diagnosis of human soft tissue sarcomas. Abstract from AEK meeting 1999, German Cancer Research Center. J Cancer Res & Clin Oncol 125 suppl (1999)
4. Than NG, Sumegi B, Than GN, Kispal B, Bohn H cloning and sequencing of human oncodevelopmental soluble placental tissue protein 17 (PP17); homology with adipophilin and the mouse adipose differentiation related protein. Tumour Biol 20, 184-192 (1999).
5. Hope RG and McLauchlan J. Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J General Virology 81, 1913-1925 (2000)
6. Ostermeyer AG, Paci JM, Zeng Y, Lublin DM, Munro S, Brown DA. Accumulation of Caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets. J Cell Biol 152, 1071-1078 (2001)
7. Fujimoto T, Kogo H, Ishiguro K, Tauchi K, Nomura R. Caveolin-2 is targeted to lipid droplets, a new "Membrane Domain" in the cell. J Cell Biol 152, 1079-1085 (2001)
Buechler C, Ritter M, Duong C Q, Orso E, Kapinsky M, Schmitz G. Adipophilin is a sensitive marker for lipid loading in human blood monocytes. Biochim Biophys Acta 1532, 97-104 (2001)
8. Hope RG, Murphy DJ, McLauchlan J. The domains required to direct core proteins of hepatitis C virus and GB Virus-B to lipid droplets share common features with plant oleosin proteins. J Biol Chem 277, 4261-4270 (2002)
9. Imamura M, Inoguchi T, Ikuyama S, Taniguchi S, Kobayashi K, Nakashima N, Nawata H. ADRP stimulates lipid accumulation and lipid droplet formation in murine fibroblasts. Am J Physiol Endocrinol Metab 283, E775-E783 (2002)
10. Fukumoto S, Fujimoto T: deformation of lipid droplets in fixed samples. Histochem Cell Biol 118, 423-428 (2002)
11. Targett-Adams P, Chambers D, Gledhill S, Hope RG, Coy JF, Girod A, McLauchlan J. Live cell analysis and targeting of the lipid droplet binding protein ADRP. J Biol Chem, Manuscript M211289200 (2003)
12. Ohsaki Y, Maeda T, Fujimoto T: Fixation and permeabilization protocol is critical for the immunolabeling of lipid droplet proteins. Histochem Cell Biol 124, 445-452 (2005)
13. Larigauderie G, Cuaz-Perolin C, Younes AB, Furman C, Lasselin C, Copin C, Jaye M, Fruchart J-C, Rouis M: Adipophilin increases triglyceride storage in human macrophages by stimulation of biosynthesis and inhibition of beta-oxidation. FEBS J 273, 3498-3510 (2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Adipophilin / ADFP (5-27) antibody

Adipophilin / ADFP Mouse IgG1 AP125 Purified Can, Hu, Rt C, ICC/IF, P, WB
50 µg / €350.00
  Acris Antibodies GmbH

Adipophilin / ADFP (N-term, 1-16) antibody

Adipophilin / ADFP Guinea Pig Serum Hu C, P, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

Adipophilin / ADFP antibody

  Mouse Monoclonal Perilipin-2/ADFP Antibody (2C5A3)  
Western Blot: ADFP Antibody (2C5A3) [TA336945] - Western blot analysis of ADFP in (1) HEK293 and (2) PLIN2 hIgGFc transfected HEK293 cell lysate. Mouse IgG1 2C5A3 Purified Hu C, E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adipophilin / ADFP antibody

  Rabbit Polyclonal Antibody against ADFP  
Detection of human ADFP in human liver lysate Rabbit IgG Purified Bov, Hu, Mky, Ms, Por P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adipophilin / ADFP (28-281) antibody

  Anti-PLIN2 Rabbit Polyclonal Antibody  
Adipophilin / ADFP Rabbit IgG Aff - Purified Bov, Hu, Ms, Por P, WB
50 µl / €400.00
  OriGene Technologies, Inc.

Adipophilin / ADFP antibody

  Anti-PLIN2 Rabbit Polyclonal Antibody  
Predicted band size: 48 kDa. Positive control: Human liver cancer and mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane2: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adipophilin / ADFP antibody

  Anti-PLIN2 Rabbit Polyclonal Antibody  
Predicted band size: 48 kDa. Positive control: Human liver cancer and mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane2: Mouse brain tissue lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adipophilin / ADFP antibody

  Rabbit Polyclonal Perilipin-2/ADFP Antibody  
Western Blot: ADFP Antibody [TA336519] - Detection of murine ADFP in mouse liver lysate. Rabbit Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Adipophilin / ADFP (C-term) antibody

Adipophilin / ADFP Guinea Pig Serum Hu C, P, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

Adipophilin / ADFP (N-term, 1-16) antibody

Adipophilin / ADFP Guinea Pig Serum Ms C, ICC/IF, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

Adipophilin / ADFP (C-term) antibody

Adipophilin / ADFP Guinea Pig Serum Ms C, ICC/IF, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

Adipophilin / ADFP (N-term, 6-27) antibody

Adipophilin / ADFP Guinea Pig Serum Hu, Ms C, P, WB
0.1 ml / €370.00
  Acris Antibodies GmbH

Adipophilin / ADFP (N-term) antibody

Adipophilin / ADFP Guinea Pig Serum Bov, Can, Hu, Ms, Rt C, P, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PLIN2 - human gene knockout kit via CRISPR

PLIN2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLIN2 - human gene knockout kit via CRISPR

PLIN2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PLIN2 - human gene knockout kit via CRISPR

PLIN2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Adipophilin / ADFP

  Recombinant protein of human adipose differentiation-related protein (ADFP)  
Adipophilin / ADFP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Adipophilin / ADFP (1-437, T7 tag)

Adipophilin / ADFP Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Adipophilin / ADFP (1-437, T7 tag)

Adipophilin / ADFP Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PLIN2 (mGFP-tagged) - Human perilipin 2 (PLIN2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PLIN2 (mGFP-tagged) - Human perilipin 2 (PLIN2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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