ADO antibody

Principal name

ADO antibody

Alternative names for ADO antibody

C10orf22, 2-aminoethanethiol dioxygenase, Cysteamine dioxygenase

SwissProt ID

Q6PDY2 (Mouse), Q96SZ5 (Human)

Gene ID

84890 (ADO)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of ADO antibody

Human thiol dioxygeses include cysteine dioxygese (CDO; MIM 603943) and cysteamine (2-aminoethanethiol) dioxygese (ADO; EC 1.13.11.19). CDO adds 2 oxygen atoms to free cysteine; whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygese activity in vitro towards cysteamine but not cysteine. Recombint Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by R-mediated interference; hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

General readings

Dominy, J.E. Jr., et al. J. Biol. Chem. 282(35):25189-25198(2007)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ADO antibody

Gel: 8%SDS-PAGE,Lysate: 40 µg,,Primary antibody: ADO Antibody at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADO antibody

Predicted band size: 30 kDa . Positive control: Mouse testis tissue lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/1200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ADO (C-term) antibody

ADO Antibody (C-term) (Cat. #AP50095PU-N) western blot analysis in mouse kidney tissue lysates (35µg/lane).This demonstrates the ADO antibody detected the ADO protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ADO antibody

ADO Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

ADO antibody

Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88529] Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

ADO antibody

Immunohistochemistry-Paraffin: ADO Antibody [NBP1-88530] Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ADO

ADO Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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