AES / ESP1 antibody

Principal name

AES / ESP1 antibody

Alternative names for AES / ESP1 antibody

GRG protein, AES1, Gp130-associated protein GAM, Amino-terminal enhancer of split

SwissProt ID

P63002 (Mouse), P63003 (Rat), Q08117 (Human)

Gene ID

166 (AES), 14797 (Aes), 29466

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunoprecipitation (IP)

Background of AES / ESP1 antibody

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).

General readings

Yochum G S., et al. (2001) Mol Cell Biol. 21:4110-4118.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AES / ESP1 (C-term) antibody

  Rabbit Polyclonal AES Antibody  
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 (N-term) antibody

  Rabbit Polyclonal AES Antibody  
Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 2 and (B) 4 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 antibody

  Goat Anti-AES Antibody  
(0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 antibody

  Rabbit Polyclonal Anti-Aes Antibody  
WB Suggested Anti-Aes Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Stomach Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 antibody

  Rabbit Polyclonal Anti-AES Antibody  
WB Suggested Anti-AES Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: DU145 cell lysateAES is supported by BioGPS gene expression data to be expressed in DU145 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 antibody

  Rabbit polyclonal Anti-Aes Antibody  
Host: Rabbit; Target Name: Aes ; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 antibody

  Rabbit Polyclonal Anti-AES Antibody  
WB Suggested Anti-AES Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat cell lysateAES is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, GP, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AES / ESP1 antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AES / ESP1 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Skeletal Muscle. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AES / ESP1 (C-term) antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human AES (SP2180P) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

AES / ESP1 antibody

Immunocytochemical staining of HeLa cells with Rabbit anti Human AES (SP2180P) Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
50 µg / €300.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

AES - human gene knockout kit via CRISPR

AES - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AES - human gene knockout kit via CRISPR

AES - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AES - human gene knockout kit via CRISPR

AES - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AES - human gene knockout kit via CRISPR

AES - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AES / ESP1 (transcript variant 2)

  Recombinant protein of human amino-terminal enhancer of split (AES), transcript variant 2  
AES / ESP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

AES / ESP1 (transcript variant 3)

  Purified recombinant protein of Homo sapiens amino-terminal enhancer of split (AES), transcript variant 3  
AES / ESP1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AES / ESP1 (1-197, His-tag)

AES / ESP1 Human Purified > 95 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH

AES / ESP1 (1-197, His-tag)

AES / ESP1 Human Purified > 95 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH

Lysates (Knockout)

Catalog No.    

AES-knockout Cell lysate

AES-knockout Cell lysate  
0.1 mg / €495.00
  OriGene Technologies, Inc.

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