AFAP1 antibody

Principal name

AFAP1 antibody

Alternative names for AFAP1 antibody

AFAP-110, Actin filament-associated protein 1, 110 kDa actin filament-associated protein

SwissProt ID

Q80YS6 (Mouse), Q8N556 (Human), Q8VH46 (Rat), Q90738 (Chick)

Gene ID

60312 (AFAP1), 70292 (Afap1), 140935

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of AFAP1 antibody

Kit Component:
- KN300952G1, Afap1 gRNA vector 1 in pCas-Guide vector
- KN300952G2, Afap1 gRNA vector 2 in pCas-Guide vector
- KN300952D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900.
2. Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16.
3. Baisden JM, Qian Y, Zot HM, et al. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity. Oncogene 2001; 20:6435-47.
Zhang J, Park SI, Artime MC, et al. AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts. J. Clin. Invest. 2007; 117:2962-73.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI2G9 (formerly 2G9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AFAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802329) Mouse IgG1 OTI2G9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 (N-term) antibody

  Rabbit Polyclonal AFAP1 Antibody  
Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AFAP1 antibody

  Anti-AFAP1 Rabbit Polyclonal Antibody  
Predicted band size: 90 kDa . Positive control: A172 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/134 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AFAP1 antibody

  Anti-AFAP1 Rabbit Polyclonal Antibody  
Predicted band size: 90 kDa . Positive control: Human brain malignant glioma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/116 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI5G2 (formerly 5G2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI5G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI2D1 (formerly 2D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI2D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI4F4 (formerly 4F4)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI4F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI3C1 (formerly 3C1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI3C1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI2A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

  AFAP1 mouse monoclonal antibody, clone OTI4A2 (formerly 4A2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI4A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 (C-term) antibody

AFAP1 Antibody (C-term) (Cat. #AP50104PU-N) western blot analysis in MDA-MB435 cell line lysates (35 µg/lane).This demonstrates the AFAP1 antibody detected the AFAP1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

AFAP1 - human gene knockout kit via CRISPR

AFAP1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AFAP1 - human gene knockout kit via CRISPR

AFAP1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AFAP1 - human gene knockout kit via CRISPR

AFAP1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AFAP1 - human gene knockout kit via CRISPR

AFAP1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Afap1 - mouse gene knockout kit via CRISPR

Afap1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Afap1 - mouse gene knockout kit via CRISPR

Afap1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Afap1 - mouse gene knockout kit via CRISPR

Afap1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Afap1 - mouse gene knockout kit via CRISPR

Afap1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AFAP1 (full length, N-term HIS tag, transcript variant 2)

  Purified recombinant protein of Human actin filament associated protein 1 (AFAP1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
AFAP1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

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