AFAP1 antibody

Principal name

AFAP1 antibody

Alternative names for AFAP1 antibody

AFAP-110, Actin filament-associated protein 1, 110 kDa actin filament-associated protein

SwissProt ID

Q80YS6 (Mouse), Q8N556 (Human), Q8VH46 (Rat), Q90738 (Chick)

Gene ID

60312 (AFAP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of AFAP1 antibody

Actin filament associated protein (AFAP-110) interacts directly with Actin filaments through its C-terminal Actin binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. AFAP-110 links signaling molecules to Actin filaments, provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. Deletion of certain binding elements of AFAP-110 results in altered Actin phenotypes; for instance, deletion of the leucine zipper motif causes repositioning of Actin into rosettes. Because inhibition of certain Actin cytoskeletal conformations inhibits cell division and movement, these conformational changes to AFAP-110, and thus Actin organization in the cell, represent a possible therapeutic target for controlling tumorigenesis and metastasis.

General readings

1. Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900.
2. Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16.
3. Baisden JM, Qian Y, Zot HM, et al. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity. Oncogene 2001; 20:6435-47.
Zhang J, Park SI, Artime MC, et al. AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts. J. Clin. Invest. 2007; 117:2962-73.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AFAP1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AFAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802329) Mouse IgG1 OTI2G9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 (N-term) antibody

Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AFAP1 antibody

Predicted band size: 90 kDa . Positive control: A172 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/134 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AFAP1 antibody

Predicted band size: 90 kDa . Positive control: Human brain malignant glioma tissue lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/116 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AFAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI5G2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI2D1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI4F4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI3C1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI2A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Mouse IgG1 OTI4A2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AFAP1 (C-term) antibody

AFAP1 Antibody (C-term) (Cat. #AP50104PU-N) western blot analysis in MDA-MB435 cell line lysates (35 µg/lane).This demonstrates the AFAP1 antibody detected the AFAP1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AFAP1 (N-term) Control Peptide

AFAP1
50 µg / €160.00
  Acris Antibodies GmbH

AFAP1 antibody

AFAP1 Mouse IgG2a 3B12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

AFAP1 antibody

Hum. Fetal Heart; Host:Rabbit. Target Name:AFAP1. Sample Tissue:Human Fetal Heart. Antibody Dilution: 1.0ug/ml.; AFAP1 antibody - middle region (ARP57552_P050) in Hum. Fetal Heart cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AFAP1 antibody

Western Blot: AFAP Antibody [NBP1-56581] - This Anti-AFAP1 antibody was used in Western Blot of Fetal Liver tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

AFAP1 antibody

Immunohistochemistry-Paraffin: AFAP Antibody [NBP1-90216] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AFAP1 (full length, N-term HIS tag, transcript variant 2)

AFAP1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

AFAP1 (250-588, His-tag)

AFAP1 Human Purified > 80 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

AFAP1 (250-588, His-tag)

AFAP1 Human Purified > 80 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

AFAP1

AFAP1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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