AGAP3 / CENTG3 antibody

Principal name

AGAP3 / CENTG3 antibody

Alternative names for AGAP3 / CENTG3 antibody

Centaurin gamma 3, MRIP-1, CRAG, Arf-GAP, GTPase, ANK repeat and PH domain-containing protein 3, MR1-interacting protein, CRAM-associated GTPase

SwissProt ID

Q8VHH5 (Mouse), Q96P47 (Human)

Gene ID

116988 (AGAP3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of AGAP3 / CENTG3 antibody

This product is one of a range of Investigative Grade antibodies, made against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues available to us. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot (see Application Notes below). However no additional claims are made for their ability to recognise native protein in any application. CENTG3, or M-RIP, is a 116 kDa protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

General readings

Surks HK, Richards CT, Mendelsohn ME.
Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA.
J Biol Chem. 2003 Dec 19;278(51):51484-93. Epub 2003 Sep 23.
PMID: 14506264

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AGAP3 / CENTG3 (C-term) antibody

TA305820 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Goat Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AGAP3 / CENTG3 antibody

Host: Rabbit; Target Name: AGAP3; Sample Tissue: 293T Whole Cell lysates; Antibody Dilution: 1.0 ug/ml. Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AGAP3 / CENTG3 (899-911) antibody

Kidney: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Prim, Rb, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

AGAP3 / CENTG3 antibody

AGAP3 / CENTG3 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

AGAP3 / CENTG3 antibody

AGAP3 / CENTG3 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

AGAP3 / CENTG3 antibody

AGAP3 / CENTG3 Goat Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

AGAP3 / CENTG3 antibody

Western Blot: CENTG3 Antibody [NBP1-22968]- Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CENTG3 antibody used for IP at 10 mcg/mg lysate. CENTG3 was also immunoprecipitated by rabbit anti-CENTG3 antibody NBP1-22969, which recognizes a downstream epitope. Rabbit IgG Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

AGAP3 / CENTG3 antibody

AGAP3 / CENTG3 Goat Hu, Ms, Rt C, E
50 µg / €480.00
  Novus Biologicals Inc.

AGAP3 / CENTG3 antibody

Immunohistochemistry-Paraffin: AGAP3 Antibody [NBP1-85685] Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AGAP3 / CENTG3 (transcript variant 1)

AGAP3 / CENTG3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AGAP3 / CENTG3

AGAP3 / CENTG3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AGAP3 / CENTG3

AGAP3 / CENTG3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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