Agrin antibody

Principal name

Agrin antibody

Alternative names for Agrin antibody

AGRN

SwissProt ID

A2ASQ1 (Mouse), O00468 (Human), P25304 (Rat), P31696 (Chick)

Gene ID

25592 (Agrn), 375790 (AGRN)

Available reactivities

Ms (Mouse), Rt (Rat), Hu (Human)

Available hosts

Mouse, Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Agrin antibody

Agrin is a neuronal aggregating factor that induces the aggregation of acetylcholine receptors and other postsynaptic proteins on muscle fibers and is crucial for the formation of the neuromuscular junction. Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

General readings

General / background references:Grow WA & Gordon H Sialic acid inhibits agrin signaling in C2 myotubes. Cell Tissue Res 299:273-9 (2000). PubMed PMID: 10741468
Hoch W Formation of the neuromuscular junction. Agrin and its unusual receptors. Eur J Biochem 265:1-10 (1999). PubMed PMID: 10491152
Groffen AJ et al. Agrin is a major heparan sulfate proteoglycan in the human glomerular basement membrane. J Histochem Cytochem 46:19-27 (1998). PubMed PMID: 9405491
Sharp AA & Caldwell JH Aggregation of sodium channels induced by a postnatally upregulated isoform of agrin. J Neurosci 16:6775-83 (1996). PubMed PMID: 8824318
Campanelli JT et al. Alternative RNA splicing that determines agrin activity regulates binding to heparin and alpha-dystroglycan. Development 122:1663-72 (1996). PubMed PMID: 8625852
Glass DJ et al. Agrin acts via a MuSK receptor complex. Cell 85:513-23 (1996). PubMed PMID: 8653787
Bowen DC et al. Neural agrin activates a high-affinity receptor in C2 muscle cells that is unresponsive to muscle agrin. J Neurosci 16:3791-7 (1996). PubMed PMID: 8656273
Hoch W et al. Structural domains of agrin required for clustering of nicotinic acetylcholine receptors. EMBO J 13:2814-21 (1994). PubMed PMID: 8026466
Ferns MJ et al. The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11:491-502 (1993). PubMed PMID: 8398142
Tsim KW et al. cDNA that encodes active agrin. Neuron 8:677-89 (1992). PubMed PMID: 1314620
Wallace BG Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus. J Neurosci 9:1294-302 (1989). PubMed PMID: 2539442

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Agrin antibody

Predicted cell location: Cytoplasm . Positive control: Human thyroid cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using AGRN antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Agrin antibody

Predicted cell location: Cytoplasm . Positive control: Human esophagus and lung cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human esophagus cancer tissue using AGRN antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Agrin antibody

Agrin Mouse IgG1 Agr247 Purified Ms, Rt C, WB
0.2 mg / €690.00
  Acris Antibodies GmbH

Agrin antibody

Western blot analysis of Agrin expression in HEK293T (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Agrin antibody

Western blot analysis of Agrin expression in HEK293T (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Agrin antibody

Western blot analysis of Agrin expression in HEK293T (A); NIH3T3 (B); PC12 (C) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Agrin antibody

Agrin Mouse IgG1 Purified Rt E, WB
0.5 mg / €410.00
  Neuromics Antibodies

Agrin antibody

Agrin Mouse IgG1 AGR 131 Ms, Rt ICC/IF, WB
0.1 mg / €380.00
  Novus Biologicals Inc.

Agrin antibody

Agrin Mouse IgG2a Agr-131 Purified Rt WB
50 µg / €380.00
  Novus Biologicals Inc.

Agrin antibody

Agrin Mouse IgG1 Agr-247 Purified Ms, Rt P, WB
50 µg / €380.00
  Novus Biologicals Inc.

Agrin antibody

Immunohistochemistry-Paraffin: Agrin Antibody [NBP1-90209] Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

AGRN overexpression lysate

AGRN overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

12 Item(s)

per page
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