AGXT / AGT1 antibody

Principal name

AGXT / AGT1 antibody

Alternative names for AGXT / AGT1 antibody

SPAT, SPT, Serine-pyruvate aminotransferase

SwissProt ID

O35423 (Mouse), P09139 (Rat), P21549 (Human)

Gene ID

189 (AGXT)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C)

Background of AGXT / AGT1 antibody

Serine—pyruvate aminotransferase is an enzyme that in humans is encoded by the AGXT gene. This gene is expressed only in the liver and the encoded protein is localized mostly in the peroxisomes; where it is involved in glyoxylate detoxification. Mutations in this gene; some of which alter subcellular targetting; have been associated with type I primary hyperoxaluria. Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate; and the progressive accumulation of insoluble calcium oxalate in the kidney and uriry tract.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AGXT / AGT1 antibody

Predicted band size: 43 kDa. Positive control: Human fetal liver tissue and hepg2 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human fetal liver tissue lysate Lane2: Hepg2 cell lysate Lysates: 40 ug per lane Primary antibody: 1/1050 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AGXT / AGT1 antibody

Predicted band size: 43 kDa. Positive control: Hela cell and human fetal liver tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Human fetal liver tissue lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AGXT / AGT1 antibody

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT2T4 Purified Hu E, ICC/IF
0.1 ml / €310.00
  Acris Antibodies GmbH

AGXT / AGT1 antibody

Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-AGXT antibody (1:500) with Alexa 488 (Green). Mouse IgG2b AT2T4 Purified Hu E, ICC/IF
50 µl / €230.00
  Acris Antibodies GmbH

AGXT / AGT1 antibody

Western blot analysis of AGXT Antibody (Center) (AP17093PU-N) in HepG2 cell line lysates (35 µg/lane). AGXT (arrow) was detected using the purified Pab.(8 µg/ml). Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AGXT / AGT1 antibody

AGXT / AGT1 Mouse IgG2b AT2T4 Aff - Purified Hu E, ICC/IF
0.1 ml / €380.00
  Novus Biologicals Inc.

AGXT / AGT1 antibody

Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AGXT / AGT1

AGXT / AGT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AGXT / AGT1

AGXT / AGT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

AGXT / AGT1

AGXT / AGT1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

AGXT overexpression lysate

AGXT overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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