Ah receptor / AhR antibody
Principal name
Ah receptor / AhR antibody
Alternative names for Ah receptor / AhR antibody
AHR, BHLHE76, Aryl hydrocarbon receptor
Ncbi ID
9606, NP_001612, NP_001612.1, AAH69390.1, NP_038492.1, NM_013464, NM_013149, NM_001621
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA)
Background of Ah receptor / AhR antibody
Aryl hydrocarbon Receptor (AhR) is a ligand-activated transcriptiol activator that binds to XRE promoter region of its target genes and has been established to activate phase I/II xenobiotic biotransformation enzyme genes. AhR binds MYBBP1A and its efficient D binding requires dimerization with another bHLH proteinsuch as ARNT. Besides interacting with NEDD8 and IVNS1ABP, AhR also interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. AhR localize to cytoplasm and upon binding with ligand followed by interaction with a HSP90, it translocates to the nucleus. AhR has been shown to be induced or repressed by TGFB1 as well as dioxin in a cell-type specific fashion, whereas cAMP, retinoic acid, TPA (12-O-tetradecanoyl phorbol-13 acetate) have been shown to exert repressive effects on AhR. As a member of bHLH-PAS (basic helix-loop-helix Per/ARNT/Sim) family, AhR has been implicated in physiological processes such as circadian cycle, neurogenesis, organs development and regulation of cell-cycle as well as the altertive sigling pathways (independently of exposure to xenobiotics). AhR- mice exhibit infertility, liver abnormalities and cardiovascular problems such as a defective closure of the ductus venosus, indicative of the implication of the AhR in various developmental processes.
General readings
Marlowe,J.L., et al., (2004) J. Biol. Chem. 279 (28), 29013-29022
Immunofluorescence of monoclonal antibody to AHR on HeLa cell. [antibody concentration 10 ug/ml]
Western blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi ( Cat # H00000196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AHR monoclonal antibody (M02), clone 3B12 (Cat # H00000196-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
AHR monoclonal antibody (M02), clone 3B12 Western Blot analysis of AHR expression in COLO 320 HSR ( Cat # L020V1 ).
Western Blot analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M02), clone 3B12.Lane 1: AHR transfected lysate(96.147 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged AHR is approximately 0.03ng/ml as a capture antibody.
Western Blot analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M03), clone 3H4.Lane 1: AHR transfected lysate(96.1 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged AHR is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of AHR expression in transfected 293T cell line by AHR monoclonal antibody (M04), clone 4G7.Lane 1: AHR transfected lysate(96.1 KDa).Lane 2: Non-transfected lysate.
Human kidney; AHR antibody - N-terminal region (ARP32243_T100) in Human kidney cells using Immunohistochemistry
HepG2; Host: Rabbit. Target Name:AHR. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:5.0ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 6%-18%.; AHR antibody - N-terminal region (ARP32243_T100) in HepG2 cells using Western Blot
Human HepG2; WB Suggested Anti-AHR Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate; AHR antibody - N-terminal region (ARP32243_T100) in Human HepG2 cells using Western Blot
Human HepG2; . Lanes: Lane1: 50 ug HepG2 nuclei extract + benzo[a]pyrene. Lane2: 50 ug HepG2 cytoplasm extract + benzo[a]pyrene. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:1000. Gene Name: AHR (Arylhydrocarbon receptor). Submitted by: Anonymous.; AHR antibody - N-terminal region (ARP31635_T100) in Human HepG2 cells using Western Blot
Human HepG2; AHR antibody - N-terminal region (ARP31635_T100) validated by WB using Human HepG2 Lysate at 2.5ug/ml , 5ug/ml.; AHR antibody - N-terminal region (ARP31635_T100) in Human HepG2 cells using Western Blot
Human Placenta; WB Suggested Anti-AHR Antibody Titration: 2.5ug/ml. ELISA Titer: 1:12500. Positive Control: Human Placenta; AHR antibody - N-terminal region (ARP31635_T100) in Human Placenta cells using Western Blot
Immunoperoxidase of monoclonal antibody to AHR on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
Western Blot analysis of AHR expression in transfected 293T cell line (H00000196-T01) by AHR MaxPab polyclonal antibody.Lane 1: AHR transfected lysate(96.10 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of AHR expression in transfected 293T cell line (H00000196-T04) by AHR MaxPab polyclonal antibody.Lane 1: AHR transfected lysate(93.28 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of AHR in mouse liver cytosol using AHR polyclonal antibody ( Cat # PAB12490 ).
Immunohistochemistry analysis of AhR antibody (Cat.-No AP20330PU-N) in paraffin-Embedded human breast carcinoma tissue.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using AhR Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells, using AhR Antibody. The lane on the right is treated with the synthesized peptide.
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical analysis of Ah Receptor (pS36) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of Ah Receptor (pS36) expression in A431 (A); HepG2 (B); NIH3T3 (C) whole cell lysates.
Detection of AhR in mouse liver cytosol
Immunohistochemistry: Aryl hydrocarbon Receptor Antibody - Staining of Aryl hydrocarbon Receptor in mouse prostate.
Immunocytochemistry/Immunofluorescence: Aryl hydrocarbon Receptor antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western blot analysis of extracts from 293 cells, using Myc (Ab-62) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using AhR (Ab-36) antibody.The picture on the right is treated with the synthesized peptide.
Western blot analysis of AhR expression in 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA326329 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
TA326720 (0.2µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-AHR Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysate
Host: Rabbit; Target Name:AHR; Sample Tissue:HepG2; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:5.0 ug/mL; Peptide Concentration: 2.0 ug/mL; Lysate Quantity: 25 ug/lane; Gel Concentration: 6%-18%
Human kidney
Western Blot: Aryl hydrocarbon Receptor Antibody [TA336259] - U937 cell nuclear protein. Image from verified customer review.
Anti-Aryl Hydrocarbon Receptor antibody IHC of human respiratory epithelium and bronchial smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Aryl Hydrocarbon Receptor antibody IHC of human respiratory epithelium and bronchial smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Aryl Hydrocarbon Receptor antibody IHC of human respiratory epithelium and bronchial smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-Aryl Hydrocarbon Receptor antibody IHC of human respiratory epithelium and bronchial smooth muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (AHR Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)
Western blot analysis of extracts from HepG2 cells, using AhR (Phospho-Ser36) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from cos-7 cells (Lane 2), using AhR (Phospho-Ser36) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue using AhR (Phospho-Ser36) antibody.The picture on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP23638PU-N | Ah receptor / AhR (N-term) antibody |
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 |
Rabbit | IgG | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µl /
€380.00
|
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| Acris Antibodies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA301733 | Ah receptor / AhR antibody |
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| Rabbit Polyclonal Antibody against Aryl hydrocarbon Receptor | |||||||
 |
Rabbit | IgG | Purified | Ms | ICC/IF, P, WB |
0.1 ml /
€416.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA313162 | Ah receptor / AhR antibody |
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| Rabbit polyclonal AhR (Ab-36) antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA313163 | Ah receptor / AhR antibody |
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| Rabbit polyclonal AhR (Ser36) antibody(Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA325211 | Ah receptor / AhR antibody |
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| Rabbit Polyclonal AhR Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326329 | Ah receptor / AhR antibody |
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| Goat Anti-AHR Antibody | |||||||
 |
Goat | Aff - Purified | Hu | P |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA326720 | Ah receptor / AhR (749-763) antibody |
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| Goat Anti-Aryl Hydrocarbon Receptor (aa749-763) Antibody | |||||||
 |
Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA329363 | Ah receptor / AhR antibody |
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| Rabbit Polyclonal anti-AHR antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA330210 | Ah receptor / AhR antibody |
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| Rabbit Polyclonal Anti-AHR Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | P, WB |
0.1 mg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA336259 | Ah receptor / AhR antibody |
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| Goat Polyclonal AHR Antibody | |||||||
![Western Blot: Aryl hydrocarbon Receptor Antibody [TA336259] - U937 cell nuclear protein. Image from verified customer review.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-128-1-W.jpg) |
Goat | Purified | Hu, Ms, Rt | EMSA, WB |
1 ml /
€393.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341084 | Ah receptor / AhR (N-term) antibody |
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| Rabbit Polyclonal Anti-AHR Antibody (N-Terminus) | |||||||
|
Rabbit | Aff - Purified | Bear, Bov, Bt, Can, Eq, GP, Hst, Hu, Mky, Prim | P |
50 µg /
€400.00
|
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| OriGene Technologies, Inc. | |||||||
| TA341085 | Ah receptor / AhR (Internal) antibody |
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| Rabbit Polyclonal Anti-AHR Antibody (Internal) | |||||||
|
Rabbit | Aff - Purified | Can, Hu, Mky, Prim | P |
50 µg /
€400.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341086 | Ah receptor / AhR (Internal) antibody |
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| Rabbit Polyclonal Anti-AHR Antibody (Internal) | |||||||
|
Rabbit | Aff - Purified | Hu, Mky, Prim | P |
50 µg /
€400.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA341087 | Ah receptor / AhR (C-term) antibody |
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| Rabbit Polyclonal Anti-AHR Antibody (C-Terminus) | |||||||
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Rabbit | Aff - Purified | Bov, Bt, Elephant, GP, Hst, Hu, Mky, Prim | P |
50 µg /
€400.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA350968 | Ah receptor / AhR antibody |
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| Rabbit Polyclonal Anti-AHR Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AM31716PU-N | Ah receptor / AhR (279-376) antibody |
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 |
Mouse | IgG2a | 3B9 | Purified | Hu | E, P |
50 µg /
€420.00
|
| Acris Antibodies GmbH | |||||||
| +1 additional image |
|||||||
| AP20330PU-N | Ah receptor / AhR antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€350.00
|
||
| Acris Antibodies GmbH | |||||||
| A0765-1 | Ah receptor / AhR pSer36 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
50 µg /
€230.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
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| A0765-2 | Ah receptor / AhR pSer36 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | E, P, WB |
0.1 mg /
€330.00
|
||
| Assay Biotech | |||||||
| +1 additional image |
|||||||
| B0765-1 | Ah receptor / AhR antibody |
||||||
 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µg /
€230.00
|
||
| Assay Biotech | |||||||
| +2 additional images |
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