AHA1 / AHSA1 antibody

Principal name

AHA1 / AHSA1 antibody

Alternative names for AHA1 / AHSA1 antibody

C14orf3, HSPC322, Activator of 90 kDa heat shock protein ATPase homolog 1

SwissProt ID

O95433 (Human), Q8BK64 (Mouse)

Gene ID

10598 (AHSA1), 217737 (Ahsa1)

Available reactivities

Hu (Human), Chimp (Chimpanzee), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Por (Porcine)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F)

Background of AHA1 / AHSA1 antibody

Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist clientprotein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370–390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.

General readings

Lenka N., et al. (1998) Prog Nucleic Acid Res Mol Biol. 61: 309-344. Schmidt TR., et al. (2002) Gene. 286: 13-19.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AHA1 / AHSA1 antibody

Western blot analysis of extracts from HT-29 cells, A549 cells and K562 cells, using AHSA1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

WB using Anti-AHA1 antibody shows detection of AHA1 in Cos7 cells. For Lanes 2 and 4, Cos7 cells were transfected with pcDNA3-FLAG-AHA1. For Lanes 1 and 3, Cos7 cells were not transfected. Extracts (40 µg per lane) were electrophoresed and transferred to nitrocellulose. The membrane was probed with anti-AHA1 (lanes 1 and 2, 1:2,000 dilution) or anti-FLAG (lanes 3 and 4). The lower band seen in anti-AHA1 blotting (arrowhead) is endogenous AHA1. Rabbit IgG Aff - Purified Chimp, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

WB using anti-AHA1 antibody shows detection of a band ~42 kDa corresponding to AHA1 in A431 whole cell lysate (lane 1) and MCF-7 whole cell lysate (lane 2). A control lane where primary ahntibody was omitted from the incubation (lane C). Molecular weight markers are shown at the left. For best results, block the membrane overnight with 3% BSA in TBS followed by reaction with primary antibody diluted 1:1,000 and use HRP conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000. Rat 25F2.D9 Purified Chimp, Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

Western blot analysis of Aha1 in HeLa cells (1) and recombinant Aha1 (2) using a 1:1000 dilution of the antibody Mouse IgG1 4H9-D7 Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

Western blot analysis of Aha1 in a human cell line mix, using a 1:1000 dilution of the antibody Rat IgG2a 25F2.D9 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

Western blot analysis of Aha1 in a human cell line mix, using a 1:1000 dilution of the antibody Rat IgG2a 25F2.D10 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

Western blot analysis of Aha1 in a rat tissue mix using a 1:1000 dilution of the antibody Rabbit Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

WB Suggested Anti-AHSA1 Antibody; Titration: 1.0 ug/ml; Positive Control: MCF7 Whole Cell AHSA1 is supported by BioGPS gene expression data to be expressed in MCF7 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

WB Suggested Anti-AHSA1 Antibody; Titration: 1.0 ug/ml; Positive Control: Jurkat Whole Cell AHSA1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AHA1 / AHSA1 antibody

Western blot analysis of Aha1 in a human cell line mix, using a 1:1000 dilution of the antibody Rat IgG2a 25F2.D9 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
25 µg / €230.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

Western blot analysis of Aha1 in a human cell line mix, using a 1:1000 dilution of the antibody Rat IgG2a 25F2.D10 Purified Hu, Ms, Rt E, ICC/IF, IP, P, WB
25 µg / €230.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COX5A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A431 cell lysate
Lane 2. : HeLa cell lysate Mouse IgG1 AT7A6 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COX5A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : A431 cell lysate
Lane 2. : HeLa cell lysate Mouse IgG1 AT7A6 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

The Recombinant Human AHA1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AHA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : AHA1 Recombinant protein
Lane 2. : MCF-7 cell lysate
Lane 3. : PC-3 cell lysate
Lane 4. : 293T cell lysate
Lane 5. : HeLa cell lysate Mouse IgG2b AT3E9 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

The Recombinant Human AHA1 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AHA1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : AHA1 Recombinant protein
Lane 2. : MCF-7 cell lysate
Lane 3. : PC-3 cell lysate
Lane 4. : 293T cell lysate
Lane 5. : HeLa cell lysate Mouse IgG2b AT3E9 Purified Hu E, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

Western blot (WB) analysis of AHA-1 antibody (Cat.-No.: AP06790PU-N) in extracts from HT-29 cells Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AHA1 / AHSA1 (N-term) antibody

Western blot analysis of AHSA1 Antibody (N-term) (AP17095PU-N) in HL60 cell line lysates (35 µg/lane). AHSA1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AHA1 / AHSA1 antibody

AHSA1 MaxPab polyclonal antibody. Western Blot analysis of AHSA1 expression in human placenta. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

AHA1 / AHSA1 antibody

AHSA1 MaxPab polyclonal antibody. Western Blot analysis of AHSA1 expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

AHA1 / AHSA1 antibody

Immunoprecipitation of AHSA1 transfected lysate using anti-AHSA1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AHSA1 MaxPab mouse polyclonal antibody (B01) (H00010598-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

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