AIF1 antibody

Principal name

AIF1 antibody

Alternative names for AIF1 antibody

AIF-1, Protein G1, IBA1, Allograft inflammatory factor 1, Ionized Calcium-Binding Adapter Molecule 1

SwissProt ID

O70200 (Mouse), P55008 (Human), P55009 (Rat), P81076 (Pig), Q9BDK2 (Bovin)

Gene ID

199 (AIF1)

Available reactivities

Hu (Human), Por (Porcine), Rt (Rat), Ms (Mouse), Can (Canine), Bov (Bovine), Eq (Equine), Gt (Goat), Hst (Hamster), Mky (Monkey), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of AIF1 antibody

Monoclonal antibody 1022-5 reacts with Allograft-inflammatory factor-1 (AIF-1) polypeptide, also known as Ionized calcium-binding adapter molecule 1, Iba-1. AIF-1 is a 17 kDa IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region. The monoclonal antibody 1022-5 is cross reactive with rat Allograft-inflammatory factor-1 (rat AIF-1). Human and rat amino acids sequences are 98% homologous.

General readings

Autieri MV & Agrawal N IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein. J Biol Chem 273:14731-7 (1998). PubMed PMID: 9614071

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AIF1 (149-161) antibody

Formalin-Fixed, Paraffin-Embedded Human Lung staining using AP08912PU-N AIF1 Antibody at 5 µg/ml after heat-induced antigen retrieval. Goat IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Gt, Hst, Hu, Mky, Ms, Platypus, Por, Prim, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AIF1 antibody

Iba1 antibody detects Iba1 protein by western blot analysis. A. 30 ug THP-1 whole cell lysate/extract. B. 30 ug HL-60 whole cell lysate/extract. 15 % SDS-PAGE. Iba1 antibody (TA308592) dilution: 1:1000 Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIF1 (Isoform 1 and 3) (C-term) antibody

TA302786 (0.5 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIF1 (Isoform 3) antibody

Anti-AIF1 (0.5µg/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIF1 (Isoform 1 and 3) antibody

TA303109 (1µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIF1 antibody

Western blot analysis of Mouse kidney tissue using TA323136(AIF1 Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIF1 antibody

Immunohistochemistry of paraffin-embedded Human gastic cancer tissue using TA323137(AIF1 Antibody)(left image) at dilution 1/50, the image on the right is treated with the fusion protein.(Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIF1 (138-148) antibody

AP08793PU-N AIF1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Placenta tissue at 2.5 µg/ml. Goat IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Fu, Gt, Hu, Mky, Pl, Por, Prim, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AIF1 antibody

Western Blot Analysis using of ant AIF1 cat# AP20480PU-N on MCF7 cells (lane 1), Mouse liver tissue lysate (lane 2) and rat heart tissue lysate (land 3) Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AIF1 (N-term) antibody

AIF1 antibody (N-term) (Cat. #AP50122PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AIF1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AIF1 antibody

BM4114 AIF1 antibody staining of Paraffin Human Spleen Section. Mouse IgG2b 1022-5 Aff - Purified Hu, Por, Rt P
0.2 mg / €570.00
  Acris Antibodies GmbH

AIF1 antibody

Figure 1. BM4114B AIF1 antibody staining of Human Spleen Paraffin Section. Mouse IgG2b 1022-5 Biotin Hu, Por, Rt P
0.1 mg / €350.00
  Acris Antibodies GmbH

AIF1 antibody

AIF1 Mouse IgG2b 1022-5 FITC Hu, Por, Rt F, P
0.2 mg / €570.00
  Acris Antibodies GmbH

AIF1 antibody

AIF1 monoclonal antibody (M01), clone 2A2-B6 Western Blot analysis of AIF1 expression in K-562 ( Cat # L009V1 ). Mouse IgG1 2A2-B6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AIF1 antibody

AIF1 polyclonal antibody ( Cat # PAB6158 ) ( 0.5µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

AIF1 antibody

AIF1 polyclonal antibody ( Cat # PAB6990 ) ( 1 µg/ml ) staining of human PBMCs lysate ( 35 µg protein in RIPA buffer ) with ( B ) and without ( A ) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

AIF1 antibody

AIF1 polyclonal antibody ( Cat # PAB7034 ) ( 1 µg/ml ) staining of human lymph node lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

AIF1 antibody

AIF1 Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €290.00
  Boster Immunoleader

AIF1 antibody

Western Blot: Iba1 Antibody - Microglial Marker [NB100-1028] - NB100-1028 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

AIF1 antibody

Immunohistochemistry-Paraffin: Iba1 Antibody [NB100-94240] - AIF in human retina with anti-AIF (IN) at 10 ug/ml. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €530.00
  Novus Biologicals Inc.

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