AIFM1 / AIF antibody

Principal name

AIFM1 / AIF antibody

Alternative names for AIFM1 / AIF antibody

PDCD8, Apoptosis-inducing factor 1 mitochondrial, Programmed cell death protein 8

SwissProt ID

O95831 (Human), O95831-2, Q9JM53 (Rat), Q9Z0X1 (Mouse)

Gene ID

51060 (TXNDC12), 9131 (AIFM1), 199 (AIF1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), Bt (Bat), Eq (Equine), Fi (Fish), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Rb (Rabbit), Xen (Xenopus), Bear, Sh (Sheep), GP (Guinea Pig)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), EPair for Elisa (EPair)

Background of AIFM1 / AIF antibody

AIF (apoptosis-inducing factor) was initially discovered as a protein involved in caspase-independent cell death. It is now known that AIF has both vital and lethal functions (reviewed in Modjtahedi et al, 2006). In healthy cells, AIF is a flavoprotein present in the mitochondria where it has vital roles in cellular redox metabolism and mitochondrial bioenergetics. In many models of apoptosis, AIF is released from the mitochondria during mitochondrial outer membrane permeabilization along with other mitochondrial proteins. Upon release, AIF translocates first to the cytosol and then to the nucleus where it induces chromatin condensation and D degradation. Although several lines of evidence suggest that AIF is a main mediator of capsase-independent cell death, the mechanisms regulating AIF pro-apoptotic function remain to be fully elucidatied and may depend on the cell type and type of apoptotic stimuli. Human AIF is transcribed from a nuclear gene located on the X chromosome and translated in the cytoplasm to a precursor protein of 613 amino acids (aa) which corresponds to ~67 kDa. The precursor protein is imported into the mitochonria by mitochondrial localization sequences located within the N-termil prodomain of AIF. Once inside the mitochondria, the prodomain is cleaved giving rise to a mature AIF form of ~57 kDa. AIF isoforms generated from a single AIF gene have been identified, including AIF short isoform 2 (324 aa protein; GenBank no. AAY84739.1) and AIF short isoform 3 (237 aa protein; GenBank noAAY84741.1 (reviewed in Delttre et al, 2006). The generation of multiple isoforms from a common gene is an evolutiory mechanism that increases protein diversiity in eukaryotes. Regulating gene expression through the production of multiple isoforms from a single gene is thought to play a major role in the control of apoptosis and other forms of programmed cell death. It recognizes AIF and AIF isoforms containing the peptide immunogen sequence, RARDPGARVLIVSEDPELP.

General readings

Zamzami N., et al. (1996) J Exp Med. 183: 1533-1544.
Susin S A., et al. (1996) J Exp Med. 184: 1331-1341.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AIFM1 / AIF antibody

Western blot analysis of extracts of various cell lines, using AIFM1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining. Mouse IgG2b 4E7 Ascites Hu, Mky, Ms, Rt E, F, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

AIFM1 / AIF (N-term) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AIFM1 / AIF antibody

Immunohistochemistry: AIF Antibody (E20) [NB110-55446] - Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using NB110-55446. Rabbit IgG E20 Supernatant Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

AIFM1 / AIF (N-term) antibody

Western blot analysis of AIF in K562 cell lysate with AIF antibody at 1 ug/mL. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 ug/ml. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF (C-term) antibody

Western blot analysis of AIF in K562 with AIF antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Western blot analysis of extracts from Jurkat cells, HepG2 cells, HUVEC cells and HeLa cells, using AIFM1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Predicted band size: 67 kDa . Positive control: NIH/3T3 , Hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 12+10%SDS-PAGE Lane1: NIH/3T3 cell lysate Lane2: Hela cell lysate Lane3: 293T cell lysate Lysates: 30 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Predicted band size: 67 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 60 ug per lane Primary antibody: 1/150 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

WB Suggested Anti-PDCD8 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysateAIFM1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

WB Suggested Anti-AIFM1 Antibody ; Titration: 1 ug/ml; Positive Control: Rat tissue Rabbit IgG Purified Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Western Blot: AIF Antibody [TA336318] - AIF levels in human neuroblastoma. Image from verified customer review. Rabbit Purified Hu, Ms, Rt C, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

Western Blot: AIF Antibody [TA337148] - Analysis of AIF in human colon using this antibody. Lane 1, normal colon. Lanes 2-7, colon carcinoma tissue lysatesfrom 6 different patients. mAIF, thought to represent mitochondrial AIF. Rabbit Purified Bov, Can, Hu, Ms, Rt C, IP, P, WB
50 µl / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

WB Suggested Anti-PDCD8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate.AIFM1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

TA348939 (0.1 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF antibody

TA348955 (0.01 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIFM1 / AIF (593-606) antibody

Figure 1. Staining AIF in Kidney tissue by Immunohistochemistry using Formalin-Fixed Paraffin-Embedded (FFPE) tissue. Rabbit Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fe, Fi, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AIFM1 / AIF (593-606) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fe, Fi, Hst, Hu, Mky, Ms, Op, Por, Prim, Rb, Rt, Xen E, ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AIFM1 / AIF Control Peptide

AIFM1 / AIF
50 µg / €160.00
  Acris Antibodies GmbH

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