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AIM2 antibody

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Principal name

AIM2 antibody

Alternative names for AIM2 antibody

Absent in melanoma 2, Interferon-inducible protein AIM2

SwissProt ID

O14862 (Human), Q91VJ1 (Mouse)

Gene ID

9447 (AIM2), 383619 (Aim2)

Ncbi ID

9606, NP_004824, NP_004824.1, AAH10940.1, NM_001013779, NM_004833

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Eq (Equine)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Immunoprecipitation (IP)

Background of AIM2 antibody

AIM2 (Absent in Melanoma-2) is an interferon-inducible cytosolic protein of the IFI20X/IFI16 family, which is essential for an effective inte immune response. It plays a key role in the defence against bacterial and viral D. AIM2 is essential for inflammasome activation in response to Francisella tularensis, vaccinia virus and Listeria monocytogenes (Ferndes-Alnemri T et al, 2009). It recognizes cytosolic dsD and forms a Caspase-1 activating inflammasome with ASC (apoptosis-associated speck-like protein containing a CARD) protein (Jones JW et al, 2010). The C-termil HIN domain binds double stranded D and acts as a cytosolic dsD sensor. The pyrin domain allows for the association with ASC and formation of the inflammasome. Formation of the AIM2 inflammsome leads to the processing and release of mature IL-1Beta and IL-18 and host cell death (Hornung V et al, 2009). AIM2 plays a role in tumorigenic reversion and may control cell proliferation. It may repress NF-kappaB transcriptiol activity.AIM2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM2 induces the expression of invasion-associated genes such as VIM and MCAM, whereas downregulates ANXA10 and CDH1. AIM2 reduces breast cancer proliferation and mammary tumor growth and shows a high frequency of frameshift mutations in microsatellite unstable (MSI-H) gastric, endometrial and colorectal cancers.Defects in AIM2expression within immune cells contribute to increased susceptibility to lupus. AIM2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients. It may serve as a potential therapeutic gene for future development of AIM2-based gene therapy for human breast cancer (Liu G et al, 2004).

General readings

Woerner,S.M., (2007) Genes Chromosomes Cancer 46 (12), 1080-1089

H00009447-B01P - AIM2
Immunofluorescence of purified MaxPab antibody to AIM2 on HeLa cell. [antibody concentration 10 ug/ml]
H00009447-B01P - AIM2
Western Blot analysis of AIM2 expression in transfected 293T cell line by AIM2 MaxPab polyclonal antibody.Lane 1: AIM2 transfected lysate(37.73 KDa).Lane 2: Non-transfected lysate.
H00009447-B02P - AIM2
Western Blot analysis of AIM2 expression in transfected 293T cell line (H00009447-T01) by AIM2 MaxPab polyclonal antibody.Lane 1: AIM2 transfected lysate(39.27 KDa).Lane 2: Non-transfected lysate.
ARP50505_P050 - AIM2
Human Lung; WB Suggested Anti-AIM2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Lung; AIM2 antibody - N-terminal region (ARP50505_P050) in Human Lung cells using Western Blot
C12049-1 - AIM2
Western blot analysis of extracts from HuvEc cells, using AIM2 Antibody. The lane on the right is treated with the synthesized peptide.
AP22454PU-N - AIM2
AP22454PU-N AIM2 antibody staining of IFNbeta-treated WI-38 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
AP22454PU-N - AIM2
AP22454PU-N AIM2 antibody staining of paraffin embedded Human Spleen at 3.8 µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
AP50126PU-N - AIM2
Western blot analysis of AIM2 Antibody (N-term) (Cat. #AP50126PU-N) in MCF-7, HL-60, K562, Hela cell line lysates (35 µg/lane). AIM2 (arrow) was detected using the purified Pab.
AP50126PU-N - AIM2
AIM2 Antibody (N-term) (Cat. #AP50126PU-N) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CPA3366-100ul - AIM2
Immunohistochemical analysis of AIM2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CPA3366-100ul - AIM2
Immunofluorescent analysis of AIM2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA3366-100ul - AIM2
Western blot analysis of AIM2 expression in Jurkat (A) whole cell lysates.
TA314913 - AIM2
Western blot analysis of extracts from HUVEC cells, using AIM2 antibody.The lane on the right is treated with the synthesized peptide.
TA314914 - AIM2
Western blot analysis of extracts from K562 cells and A549 cells, using CSRL1 antibody.The lane on the right is treated with the synthesized peptide.
TA314914 - AIM2
Immunofluorescence analysis of A549 cells, using CSRL1 antibody.The picture on the right is treated with the synthesized peptide.
TA324972 - AIM2
AIM2 Antibody(Cat. #TA324972) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the AIM2 antibody detected the AIM2 protein (arrow).
TA326327 - AIM2
TA326327 (0.3µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA328184 - AIM2
LCK Antibody (Cat. #TA328184) western blot analysis in HL-60,Jurkat,NCI-H292 cell line lysates (35ug/lane).This demonstrates the LCK antibody detected the LCK protein (arrow).
TA337162 - AIM2
Western Blot: AIM2 Antibody (10M5G5) [TA337162] - Analysis using AIM2 antibody. Recombinant human AIM2 probed with AIM2 antibody at 2 ug/ml.
TA337162 - AIM2
Western Blot: AIM2 Antibody (10M5G5) [TA337162] - Western blot analysis of Daudi lysate (35 ug per lane, RIPA buffer) using AIM2 antibody (TA337162) at 0.1ug/ml. Band observed at ~38kDa. (Expected MW of 39.0kDa according to NP_004824.1).
TA337163 - AIM2
Western Blot: AIM2 Antibody (10M2B3) [TA337163] - analysis using AIM2 antibody. Recombinant human AIM2 probed with AIM2 antibody at 2 ug/ml.
TA337163 - AIM2
Western Blot: AIM2 Antibody (10M2B3) [TA337163] - Western blot analysis of Daudi lysate (35 ug per lane, RIPA buffer) using AIM2 antibody (TA337163) at 0.3ug/ml. Band observed at ~38kDa. (Expected MW of 39.0kDa according to NP_004824.1)
TA339684 - AIM2
WB Suggested Anti-AIM2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Lung
TA352400 - AIM2
HL-60 cells were treated with human IFN? recombinant protein at 10 ng/ml for 16 hours. The untreated cells were used as the negative control. Cell lysates from control cells and the hIFN? treated cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-AIM2 antibody (clone 3B10). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
TA349676 - AIM2
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (AIM2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
TA349676 - AIM2
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (AIM2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AIM2 (C-term) antibody
AP22454PU-N AIM2 antibody staining of IFNbeta-treated WI-38 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+1 additional image

Acris recommends this product for Western blotting.

AIM2 antibody
  Rabbit polyclonal anti-AIM2 antibody  
Western blot analysis of extracts from HUVEC cells, using AIM2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIM2 antibody
  Rabbit polyclonal anti-CSRL1 antibody  
Western blot analysis of extracts from K562 cells and A549 cells, using CSRL1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

AIM2 antibody
  Mouse monoclonal AIM2 Antibody  
AIM2 Antibody(Cat. #TA324972) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the AIM2 antibody detected the AIM2 protein (arrow). Mouse IgM 812CT9.2.2.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AIM2 antibody
  Goat Anti-AIM2 Antibody  
TA326327 (0.3µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AIM2 antibody
  Mouse monoclonal anti-AIM2 antibody(Ascites)  
LCK Antibody (Cat. #TA328184) western blot analysis in HL-60,Jurkat,NCI-H292 cell line lysates (35ug/lane).This demonstrates the LCK antibody detected the LCK protein (arrow). Mouse IgM 812CT9.2.2.1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AIM2 antibody
  Mouse Monoclonal AIM2 Antibody (10M5G5)  
Western Blot: AIM2 Antibody (10M5G5) [TA337162] - Analysis using AIM2 antibody. Recombinant human AIM2 probed with AIM2 antibody at 2 ug/ml. Mouse IgG2a 10M5G5 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

AIM2 antibody
  Mouse Monoclonal AIM2 Antibody (10M2B3)  
Western Blot: AIM2 Antibody (10M2B3) [TA337163] - analysis using AIM2 antibody. Recombinant human AIM2 probed with AIM2 antibody at 2 ug/ml. Mouse IgG2a 10M2B3 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

AIM2 antibody
  Rabbit Polyclonal Anti-AIM2 Antibody  
WB Suggested Anti-AIM2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Lung Rabbit IgG Purified Eq, Hu, Ms, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

AIM2 antibody
  Rabbit Polyclonal Anti-AIM2 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (AIM2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

AIM2 antibody
  Mouse Monoclonal anti-AIM2 Antibody  
HL-60 cells were treated with human IFN? recombinant protein at 10 ng/ml for 16 hours. The untreated cells were used as the negative control. Cell lysates from control cells and the hIFN? treated cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with mouse anti-AIM2 antibody (clone 3B10). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. Mouse 3B10 Purified Hu ICC/IF, IP, WB
0.1 mg / €280.00
  OriGene Technologies, Inc.

AIM2 antibody
  Rabbit Polyclonal AIM2 Antibody  
AIM2 Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

AIM2 (N-term) antibody
Western blot analysis of AIM2 Antibody (N-term) (Cat. #AP50126PU-N) in MCF-7, HL-60, K562, Hela cell line lysates (35 µg/lane). AIM2 (arrow) was detected using the purified Pab. Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €370.00
  Acris Antibodies GmbH
+1 additional image

AIM2 antibody
Western blot analysis of extracts from HuvEc cells, using AIM2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €230.00
  Assay Biotech

AIM2 antibody
Western blot analysis of extracts from HuvEc cells, using AIM2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

AIM2 - human gene knockout kit via CRISPR
AIM2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AIM2 - human gene knockout kit via CRISPR
AIM2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AIM2 - human gene knockout kit via CRISPR
AIM2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AIM2 - human gene knockout kit via CRISPR
AIM2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, AIM2 (mGFP-tagged)-Human absent in melanoma 2 (AIM2), 200 uL, >10^7 TU/mL
Lenti ORF particles, AIM2 (mGFP-tagged)-Human absent in melanoma 2 (AIM2), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.
 

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