AKAP12 / SSeCKS antibody

Principal name

AKAP12 / SSeCKS antibody

SwissProt ID

Q62766 (Rat)

Ncbi ID

10116

Available reactivities

Bov (Bovine), Rt (Rat)

Available hosts

Sheep

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB)

Background of AKAP12 / SSeCKS antibody

Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for SSeCKS, a PKA/PKC scaffolding protein, has been implicatedduring the process of spermiogenesis and in the actinbased stellate morphology of mesangial cells.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKAP12 / SSeCKS antibody

Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/5%NFDM, probed with 5 μg/ml of pre-immune serum 5 µg of SSeCKS antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-Sheep IgG coupled to HRP 1/10,000, and detected with Luminol. Sheep IgG Purified Bov, Rt C, IP, WB
0.25 mg / €370.00
  Acris Antibodies GmbH

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