AKAP12 antibody

Principal name

AKAP12 antibody

Alternative names for AKAP12 antibody

AKAP250, Gravin, PRKA12, A-kinase anchor protein 12, A-kinase anchor protein 250 kDa, Myasthenia gravis autoantigen

SwissProt ID

Q02952 (Human), Q5QD51 (Rat), Q9WTQ5 (Mouse)

Gene ID

9590 (AKAP12), 83397 (Akap12)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of AKAP12 antibody

Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. Antibodies to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKAP12 antibody

Sample (30ug whole cell lysate). A:HeLa S3. 5% SDS PAGE. TA307903 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKAP12 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP12 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKAP12 antibody

Predicted cell location: Cytoplasm . Positive control: Human brain and lung cancer tissue . Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using AKAP12 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKAP12 antibody

Predicted band size: 191 kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKAP12 antibody

ELISA: AKAP12 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

AKAP12 (C-term) antibody

AKAP12 Goat Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AKAP12 antibody

AKAP12 Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

AKAP12 antibody

Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2b 1C5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKAP12 antibody

Detection limit for recombinant GST tagged AKAP12 is approximately 3ng/ml as a capture antibody. Mouse IgG2b 3C3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKAP12 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP12 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
50 µg / €205.00
  Assay Biotech

AKAP12 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using AKAP12 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P
0.1 mg / €310.00
  Assay Biotech

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AKAP12

AKAP12 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AKAP12

AKAP12 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

13 Item(s)

per page
  • LinkedIn