AKB ligase / GCAT antibody

Principal name

AKB ligase / GCAT antibody

Alternative names for AKB ligase / GCAT antibody

Glycine acetyltransferase, Aminoacetone synthetase, KBL, 2-amino-3-ketobutyrate coenzyme A ligase

SwissProt ID

O75600 (Human), Q0P5L8 (Bovin)

Gene ID

23464 (GCAT)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of AKB ligase / GCAT antibody

GCAT is also known as KBL, aminoacetone synthase or 2-amino-3-ketobutyrate coenzyme A ligase and is a 419 amino acid protein. GCAT is localized to mitochondria in various tissues, including heart, brain, liver, pancreas and lungs. In mitochondria, two enzymes function in catalyzing the reaction that converts L-threonine into glycine, the second of which is GCAT. L-threonine is first converted into 2-amino-3-ketobutyrate by TDH, a reaction that is proceeded by the CoA- and GCAT-dependent formation of glycine and acetyl-CoA. Defects in the gene encoding GCAT may result in elevated levels of aminoacetone and carbon dioxide, both of which are products of the 2-amino-3-ketobutyrate intermediate and can further breakdown to form methylglyoxal, hydrogen peroxide and ammonia. Excess blood levels of methylglyoxal may cause kidney damage and are thought to be associated with diabetic complications.

General readings

Edgar A.J., et al. (2000) Eur. J. Biochem. 267:1805-1812Hoshino A., et al. (2007) Cell Stress Chaperones. 12:186-191

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKB ligase / GCAT (N-term) antibody

Western blot analysis of GCAT (arrow) using AKB ligase / GCAT Antibody (N-term) Cat.-No AP51795PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GCAT gene. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AKB ligase / GCAT antibody

Immunohistochemistry-Paraffin: GCAT Antibody [NBP1-81564] Immunohistochemical staining of human small intestine shows distinct positivity in mucus of goblet cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AKB ligase / GCAT (full length, N-term HIS tag)

AKB ligase / GCAT Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

AKB ligase / GCAT (22-419, His-tag)

AKB ligase / GCAT Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

AKB ligase / GCAT (22-419, His-tag)

AKB ligase / GCAT Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

AKB ligase / GCAT

AKB ligase / GCAT Human
2 µg / €300.00
  Abnova Taiwan Corp.

AKB ligase / GCAT

AKB ligase / GCAT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AKB ligase / GCAT

AKB ligase / GCAT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

8 Item(s)

per page
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