AKR1B10 antibody

Principal name

AKR1B10 antibody

Alternative names for AKR1B10 antibody

AKR1B11, Aldose reductase-like, ARL-1, Aldose reductase-related protein, Small intestine reductase, Aldo-keto reductase family 1 member B10

SwissProt ID

O60218 (Human)

Gene ID

57016 (AKR1B10)

Available reactivities

Hu (Human), Ms (Mouse), Dros (Drosophila), Rt (Rat), Ze (Zebrafish), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)

Background of AKR1B10 antibody

AKR1B10 (aldo-keto reductase 1B10) is a monomeric enzyme that reduces aliphatic and aromatic aldehydes as well as certain hexoses thereby playing a critical role in detoxification of dietary/cellular alpha or beta-unsaturated carbonyls and lipid peroxides (LPOs) ultimately leading to host cells protection against carbonyl lesions. AKR1B10 is active against polycyclic aromatic hydrocarbon (PAHs) which are potent procarcinogens and against all-trans-retil, the sigling molecule retinoic acid's precursor which regulates cellular proliferation and differentiation. AKR1B10 is a cytosolic protein, but gets secreted through lysosome-mediated mechanisms and in normal tissues, it is expressed in colon, small intestine, adrel gland etc., whereas its expression gets upregulated in lung bronchial epithelium of cigarette smokers further augmenting the risk of PAHs mediated initiation of carcinogenesis. Moreover, AKR1B10 is overexpressed in certain cancers such as liver, breast and lung cancer, and because of its reducing potential for C13 ketonic groups of daunorubicin and idarubicin, it may provide chemoresistance for cancer cells to these drugs. Moreover, upregulated AKR1B10 in malignt cells blocks ubiquitin-dependent degradation of ACCA (acetyl-CoA carboxylase-alpha) thereby promoting fatty acid/lipid synthesis which is essential to cancer cell growth and proliferation.

General readings

Tammali R, Ramana KV, Singhal SS, Awasthi S, Srivastava SK.
Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells.
Cancer Res. 2006 Oct 1;66(19):9705-13.
PMID: 17018629

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKR1B10 antibody

AKR1B10 antibody detects AKR1B10 protein by Western blot analysis. A. 50 ug mouse colon lysate/extract. 10 % SDS-PAGE. AKR1B10 antibody (TA308188) dilution: 1:5000 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1B10 antibody

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1A6 Purified Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

AKR1B10 antibody

Immunoperoxidase of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml] Mouse IgG2a 1A6 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1B10 (C-term) antibody

TA302407 (0.03µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKR1B10 antibody

Predicted band size: 36kDa. Positive control: Raji and Jurkat cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: Raji cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/175 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1B10 antibody

Predicted band size: 36kDa. Positive control: A549 cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/184 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1B10 antibody

Western Blot: AKR1B10 Antibody [TA336761] - AKR1B10 expression in 1) aldose reductase (AR) protein, 2) AKR1B10 protein, 3) 293T cells expressing AR and 4) HCT-8 cells expressing AKR1B10. Image courtesy of the laboratory of Dr. Deliang Cao at Southern Illi Rabbit Purified Hu, Ms C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1B10 antibody

WB Suggested Anti-AKR1B10 Antibody Titration: 1.25 ug/ml; Positive Control: A549 cell lysate; AKR1B10 is supported by BioGPS gene expression data to be expressed in A549 Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKR1B10 (100-200) antibody

Colon: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AKR1B10 antibody

AKR1B10 polyclonal antibody (A01), Lot # UOP11060207QCS1 Western Blot analysis of AKR1B10 expression in K-562 ( Cat # L009V1 ). Mouse Serum Hu, Ms E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

AKR1B10 antibody

Western Blot analysis of AKR1B10 expression in transfected 293T cell line (H00057016-T01) by AKR1B10 MaxPab polyclonal antibody.

Lane 1: AKR1B10 transfected lysate(34.76 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

AKR1B10 antibody

AKR1B10 Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

AKR1B10 antibody

AKR1B10 monoclonal antibody (M03), clone 2B3. Western Blot analysis of AKR1B10 expression in HepG2 ( Cat # L019V1 ). Mouse IgG1 2B3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1B10 antibody

AKR1B10 polyclonal antibody ( Cat # PAB7448 ) ( 0.03 µg/ml ) staining of A-549 cell lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

AKR1B10 antibody

Human A549; WB Suggested Anti-AKR1B10 Antibody Titration: 1.25ug/ml. Positive Control: A549 cell lysate. AKR1B10 is supported by BioGPS gene expression data to be expressed in A549.; AKR1B10 antibody - N-terminal region (ARP42553_T100) in Human A549 cells using Western Blot Rabbit Purified Dros, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

AKR1B10 antibody

Western Blot: AKR1B10 Antibody [NB100-93370]- HEK293 overexpressing AKR1B10 and probed with EB08336 (mock transfection in first lane). Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

AKR1B10 antibody

AKR1B10 Rabbit Aff - Purified Hu C, ICC/IF, P, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

AKR1B10 antibody

AKR1B10 Rabbit Aff - Purified Hu C, E, ICC/IF, IP, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

AKR1B10 antibody

AKR1B10 Rabbit Aff - Purified Hu C, E, ICC/IF, IP, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

AKR1B10 antibody

AKR1B10 Rabbit Aff - Purified Hu C, ICC/IF, P, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

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