AKR1C1 / DHH1 antibody

Principal name

AKR1C1 / DHH1 antibody

Alternative names for AKR1C1 / DHH1 antibody

20-alpha-hydroxysteroid dehydrogenase, Aldo-keto reductase family 1 member C1, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, Dihydrodiol dehydrogenase 1/2, Chlordecone reductase homolog HAKRC, High-affinity hepatic bile acid-binding protein

SwissProt ID

Q04828 (Human)

Gene ID

1645 (AKR1C1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit), GP (Guinea Pig), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Frozen Sections (C), Flow Cytometry (F), Immunoprecipitation (IP)

Background of AKR1C1 / DHH1 antibody

DD1 is also designated AKR1C1, DDH or DDH1, while DD2 also can be designated AKR1C2, dDD, BABP or DDH2. AKR1C3 and 3alpha-HSD are alternate designations for human DD3 (which is referred to as AKR1C18 in rodents), while DD4 also can be called AKR1C4, CD, CHDR or AKR1C6 (in rodents). DD1 and DD2 are 20alpha-HSDs, whereas DD3 and DD4 are the 3alpha-HSDs. The multiple human cytosolic dihydrodiol dehydrogenases are involved in the metabolism of xenobiotics, such as polycyclic aromatic hydrocarbons, pesticides and steroid hormones, and are responsible for the reduction of ketone-containing drugs by using NADH or NADPH as a cofactor. The 20alpha-HSD catalyzes the reaction of progesterone to the inactive form 20alpha-hydroxyprogesterone. The 3alpha-HSD is a cytosolic, monomeric, NADPH-dependent oxidoreductase that reduces 3-keto-5-dihydrosteroids to their tetrahydro products. DD1 and DD2 are ubiquitously expressed, whereas DD4 mRNA is restricted to the liver. DD3 is a unique enzyme that can specifically catalyze the dehydrogenation of trans-benzenedihydrodiol and trans-naphthalenedihydrodiol.

General readings

Zhang Y., et al. (2000) J. Mol. Endocrinol. 25:221-228.
Zhang Y., et al. (2009) Mol Cell Endicrinol. 298(1-2):76-83.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKR1C1 / DHH1 antibody

Sample(30 ug whole cell lysate). A:Raji . 10% SDS PAGE. TA308032 diluted at 1:1000 Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1C1 / DHH1 (1-323) antibody

Western blot analysis: The extracts of HepG2 (33 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT6D10 Purified Hu E, F, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

AKR1C1 / DHH1 (1-323) antibody

Western blot analysis: The extracts of HepG2 (33 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKR1C1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT6D10 Purified Hu E, F, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

AKR1C1 / DHH1 antibody

Predicted band size: 37 kDa. Positive control: Human liver cancer and breast infiltrative duct tissue, human fetal brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane1: Human breast infiltrative duct tissue lysate Lane1: Human fetal brain tissue lysate Lysate: 27 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1C1 / DHH1 antibody

Predicted cell location: Cytoplasm . Positive control: Human breast and thyroid cancer tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using AKR1C1 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1C1 / DHH1 antibody

WB Suggested Anti-AKR1C1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AKR1C1 / DHH1 antibody

WB Suggested Anti-AKR1C1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh WB
50 µg / €325.00
  OriGene Technologies, Inc.

AKR1C1 / DHH1 antibody

AKR1C1 polyclonal antibody (A01), Lot # 070510JCS1 Western Blot analysis of AKR1C1 expression in HeLa ( Cat # L013V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

AKR1C1 / DHH1 antibody

AKR1C1 MaxPab polyclonal antibody. Western Blot analysis of AKR1C1 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

AKR1C1 / DHH1 antibody

Western Blot analysis of AKR1C1 expression in transfected 293T cell line (H00001645-T02) by AKR1C1 MaxPab polyclonal antibody.

Lane 1: AKR1C1 transfected lysate(36.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

AKR1C1 / DHH1 antibody

AKR1C1 MaxPab rabbit polyclonal antibody. Western Blot analysis of AKR1C1 expression in human liver. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

AKR1C1 / DHH1 antibody

AKR1C1 / DHH1 Mouse IgG2a 1B3 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1C1 / DHH1 (N-term) antibody

AKR1C1 / DHH1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AKR1C1 / DHH1 (N-term) antibody

AKR1C1 / DHH1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AKR1C1 / DHH1 antibody

Immunohistochemical analysis of AKR1C1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKR1C1 / DHH1 antibody

Immunohistochemical analysis of AKR1C1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKR1C1 / DHH1 antibody

Immunohistochemical analysis of AKR1C1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKR1C1 / DHH1 antibody

AKR1C1 / DHH1 Mouse IgG1 X 197 Hu C, F, P, WB
50 µg / €500.00
  Novus Biologicals Inc.

AKR1C1 / DHH1 antibody

AKR1C1 / DHH1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

AKR1C1 / DHH1 antibody

AKR1C1 / DHH1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

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