AKR1D1 / SRD5B1 antibody

Principal name

AKR1D1 / SRD5B1 antibody

Alternative names for AKR1D1 / SRD5B1 antibody

3-oxo-5-beta-steroid 4-dehydrogenase, Delta(4)-3-ketosteroid 5-beta-reductase, Delta(4)-3-oxosteroid 5-beta-reductase, Aldo-keto reductase family 1 member D1

SwissProt ID

P31210 (Rat), P51857 (Human), Q8VCX1 (Mouse)

Gene ID

6718 (AKR1D1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P)

Background of AKR1D1 / SRD5B1 antibody

Aldo-keto reductase family 1, member D1, also known as AKR1D1, is a member of the AKR superfamily. The AKR family of proteins are soluble NADPH oxidoreductases. They play important roles in the metabolism of drugs, carcinogens and reactive aldehydes. AKR1D1 is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones which carry a delta (4)-3-one structure. AKR1D1 is highly expressed in liver, colon and testis. Deficiency of this enzyme may contribute to hepatic dysfunction. Recombinant human AKR1D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

1. Charbonneau A., et al. (2001) Biochim Biophys Acta. 1517:228-235.
2. Lemonde H A., et al. (2003) Gut. 52:1494-1499.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKR1D1 / SRD5B1 antibody

Predicted band size: 37 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 antibody

Predicted band size: 37 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 antibody

WB Suggested Anti-AKR1D1 Antibody; Titration: 1.0 ug/ml; Positive Control: RPMI-8226 Whole Cell Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 (Center) antibody

AKR1D1 Antibody (Center) (Cat. #AP50135PU-N) western blot analysis in 293 cell line lysates (35 µg/lane).This demonstrates the AKR1D1 antibody detected the AKR1D1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 antibody

AKR1D1 MaxPab polyclonal antibody. Western Blot analysis of AKR1D1 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line (H00006718-T01) by AKR1D1 MaxPab polyclonal antibody.

Lane 1: AKR1D1 transfected lysate(37.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

AKR1D1 / SRD5B1 Mouse IgG2b 1A6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2.

Lane 1: AKR1D1 transfected lysate(37.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 1C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

Human RPMI-8226; WB Suggested Anti-AKR1D1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: RPMI-8226 Whole Cell; AKR1D1 antibody - middle region (ARP60804_P050) in Human RPMI-8226 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

AKR1D1 / SRD5B1 antibody

AKR1D1 / SRD5B1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 (1-326, His-tag)

Recombinant human AKR1D1, 1-326 aa, His-tagged Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 (1-326, His-tag)

Recombinant human AKR1D1, 1-326 aa, His-tagged Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 (1-326)

AKR1D1 / SRD5B1 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 (1-326)

AKR1D1 / SRD5B1 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified
0.1 mg / €380.00
  Novus Biologicals Inc.

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