AKR1D1 / SRD5B1 antibody

Principal name

AKR1D1 / SRD5B1 antibody

Alternative names for AKR1D1 / SRD5B1 antibody

3-oxo-5-beta-steroid 4-dehydrogenase, Delta(4)-3-ketosteroid 5-beta-reductase, Delta(4)-3-oxosteroid 5-beta-reductase, Aldo-keto reductase family 1 member D1

SwissProt ID

P31210 (Rat), P51857 (Human), Q8VCX1 (Mouse)

Gene ID

6718 (AKR1D1), 208665 (Akr1d1), 192242

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Paraffin Sections (P)

Background of AKR1D1 / SRD5B1 antibody

Kit Component:
- KN301096G1, Akr1d1 gRNA vector 1 in pCas-Guide vector
- KN301096G2, Akr1d1 gRNA vector 2 in pCas-Guide vector
- KN301096D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Moore S A., et al. (1995) J Mol Biol. 249(1):195-214Gould S J., et al. (1988) Anal Biochem. 175(1):5-13

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKR1D1 / SRD5B1 antibody

  Anti-AKR1D1 rabbit polyclonal antibody  
Predicted band size: 37 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 antibody

  Anti-AKR1D1 rabbit polyclonal antibody  
Predicted band size: 37 kDa . Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 80 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 antibody

  Rabbit Polyclonal Anti-AKR1D1 Antibody  
WB Suggested Anti-AKR1D1 Antibody; Titration: 1.0 ug/ml; Positive Control: RPMI-8226 Whole Cell Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 (Center) antibody

AKR1D1 Antibody (Center) (Cat. #AP50135PU-N) western blot analysis in 293 cell line lysates (35 µg/lane).This demonstrates the AKR1D1 antibody detected the AKR1D1 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 antibody

AKR1D1 MaxPab polyclonal antibody. Western Blot analysis of AKR1D1 expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line (H00006718-T01) by AKR1D1 MaxPab polyclonal antibody.

Lane 1: AKR1D1 transfected lysate(37.40 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

AKR1D1 / SRD5B1 Mouse IgG2b 1A6 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

Western Blot analysis of AKR1D1 expression in transfected 293T cell line by AKR1D1 monoclonal antibody (M03), clone 1C2.

Lane 1: AKR1D1 transfected lysate(37.4 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2b 1C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1 antibody

Human RPMI-8226; WB Suggested Anti-AKR1D1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: RPMI-8226 Whole Cell; AKR1D1 antibody - middle region (ARP60804_P050) in Human RPMI-8226 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

Akr1d1 - mouse gene knockout kit via CRISPR

Akr1d1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Akr1d1 - mouse gene knockout kit via CRISPR

Akr1d1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Akr1d1 - mouse gene knockout kit via CRISPR

Akr1d1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Akr1d1 - mouse gene knockout kit via CRISPR

Akr1d1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

AKR1D1 / SRD5B1

  Recombinant protein of human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1)  
AKR1D1 / SRD5B1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

AKR1D1 / SRD5B1 (1-326, His-tag)

Recombinant human AKR1D1, 1-326 aa, His-tagged Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 (1-326, His-tag)

Recombinant human AKR1D1, 1-326 aa, His-tagged Human Purified > 90 % E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 (1-326)

AKR1D1 / SRD5B1 Human Purified > 95 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1 (1-326)

AKR1D1 / SRD5B1 Human Purified > 95 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

AKR1D1 / SRD5B1

AKR1D1 / SRD5B1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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