AKT1 / PKB antibody

Principal name

AKT1 / PKB antibody

Alternative names for AKT1 / PKB antibody

Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase

SwissProt ID

P31749 (Human), P31750 (Mouse), P47196 (Rat), Q01314 (Bovin)

Gene ID

207 (AKT1), 11651 (Akt1), 24185

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), African clawed frog, Chimp (Chimpanzee), Rb (Rabbit), Mky (Monkey), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F), EPair for Elisa (EPair), Luminex (LMNX)

Background of AKT1 / PKB antibody

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.

General readings

Beffert, U., et al. (2002) Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. J. Biol. Chem. 277(51):49958-49964.
Hsu, J., et al. (2001) The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9):2853-2855.
Persad, S., et al. (2001) Regulation of protein kinase B/Akt-threonine-308 phosphorylation by integrin linked kinase (ILK): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. J. Biol. Chem. 276(29):27462-27469.
Nelson, J.M. and D.W. Fry. (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. J. Biol. Chem. 276(18):14842-14847.
Genot, E.M., et al. (2000) The T-Cell receptor regulates Akt (Protein Kinase B) via a pathway involving Rac1 and phosphotidylinositide 3-Kinase. Mol. Cell. Biol. 20(15):5469-5478.
Datta, S.R., et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91(2):231-241
del Peso, L., et al. (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278(5338):687-689
Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 275(5300):661-665
Franke, T.F., et al. (1997) PI3K: downstream AKTion blocks apoptosis. Cell 88(4):435-437

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT (pan) antibody

rhAkt1, rhAkt2, rhAkt3 (all 5 ng; lower panel), and lysates of human MCF-7, MDA-MB-123, and mouse TS1 cells (upper panel) were resolved by SDS-PAGE. Following electrophoresis, recombinant proteins and lysates were transferred to an Immobilon-P membrane and immunoblotted with 0.2 μg/mL anti-pan Akt, as described in Protocols for Immunoblotting. A fifteen second exposure to film is shown. Mouse IgG2b 281046 Purified Hu, Ms, Rt C, E, F, ICC/IF, WB
0.1 mg / €340.00
  Neuromics Antibodies

AKT1/AKT2 antibody

Thymus, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AKT1/AKT2/AKT3 (C-term) antibody

  Rabbit polyclonal anti-AKT antibody  
WB of detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red)at 1:10,000. Rabbit Serum Chk, Hu, Ms, Rt C, E, ICC/IF, IP, P, WB
0.2 ml / €387.00
  OriGene Technologies, Inc.

AKT1/AKT2/AKT3 (C-term) antibody

AP22624PU-N AKT antibody staining of Formalin-Fixed Paraffin-Embedded Human Liver at 1/100 followed by Biotin conjugated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Serum Chk, Hu, Ms, Rt E, ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

AKT1/AKT2/AKT3 antibody

  Mouse monoclonal anti-AKT antibody  
WB of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none. Mouse IgG 14E5.16C8.25F6 Purified Chimp, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

AKT1/AKT2/AKT3 pTyr315/316/312 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) Antibody (#AP08030PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
0.1 ml / €320.00
  Acris Antibodies GmbH

AKT1/AKT2/AKT3 pTyr315/316/312 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) Antibody (#AP08030PU). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
50 µl / €240.00
  Acris Antibodies GmbH

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI4D6 (formerly 4D6)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504230) Mouse IgG1 OTI4D6 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB antibody

  Sheep polyclonal anti-AKT antibody  
Western Blot of Rabbit Anti-AKT Antibody. Lane 1: HeLa cells. Lane 2: H2O2 treated MCF7 cells. Lane 3: Recombinant AKT. Lane 4: A431 cells. Load: 35 µg per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4°C. Secondary antibody: IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO overnight at 4°C. Predicted/Observed size: ~55-60 kDa for AKT . Other band(s): unspecific. Sheep IgG Aff - Purified Chk, Hu, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI4E10 (formerly 4E10)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504234) Mouse IgG1 OTI4E10 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB non pSer129 antibody

  Rabbit polyclonal Akt (Ab-129) antibody  
Western blot analysis of extracts from HuvEc cells, treated with Serum (30%, 30mins), using Akt (Ab-129) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT1 / PKB antibody

  Rabbit polyclonal Akt (Ser473) antibody(Phospho-specific)  
Western blot analysis of extracts from K562, using Akt (Phospho-Ser473) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT1 / PKB pSer473 antibody

  Rabbit polyclonal AKT pS473 antibody  
Western Blot of Rabbit anti-AKT pS473 antibody. Lane 1: nuclear extract from cells infected with adenovirus expressing nuclear-targeted AKT kinase. Load: 35 µg per lane. Primary antibody: AKT pS473 antibody at 1:200 dilution for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~56 kDa for AKT pS473. Other band(s): unspecific. Rabbit Aff - Purified Chk, Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

AKT1 / PKB pSer473 antibody

  Mouse monoclonal Akt phospho S473 antibody  
WB of Mouse Anti-AKT pS473 antibody. Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT (indicated by arrowhead at ~56 kDa) on PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT pS473 antibody at 1:10,000 in TBS with 0.05% Tween-20 with 1% BSA, for 1 h at 4° C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:20,000 dilution. Mouse IgG1 17F6.B11 Purified Chimp, Hu, Ms, Rt E, ICC/IF, IP, P, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI3E3 (formerly 3E3)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504231) Mouse IgG1 OTI3E3 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504232) Mouse IgG1 OTI4G4 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI3B4 (formerly 3B4)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504233) Mouse IgG1 OTI3B4 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI4C11 (formerly 4C11)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504235) Mouse IgG1 OTI4C11 Purified Can, Hu, Mky, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB antibody

  AKT1 mouse monoclonal antibody, clone OTI1G5 (formerly 1G5)  
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504238) Mouse IgG1 OTI1G5 Purified Can, Hu, Ms, Rt F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT1 / PKB antibody

  Rabbit Polyclonal AKT1 Antibody  
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-AKT1 rabbit polyclonal antibody. (TA590196) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt Dot, E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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