AKT1 / PKB antibody

Principal name

AKT1 / PKB antibody

Alternative names for AKT1 / PKB antibody

Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase

SwissProt ID

P31749 (Human), P31750 (Mouse), P47196 (Rat), Q01314 (Bovin)

Gene ID

207 (AKT1), 11651 (Akt1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Por (Porcine), African clawed frog, Xen (Xenopus), Chimp (Chimpanzee), Rb (Rabbit), Mky (Monkey), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse, Chicken, Sheep

Available applications

Frozen Sections (C), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F), EPair for Elisa (EPair), Luminex (LMNX)

Background of AKT1 / PKB antibody

Cell signaling mechanisms often transmit information via posttranslational protein modifications, most importantly reversible protein phosphorylation mediated by protein kinases. AKT (also called protein kinase B) is a serine-threonine kinase that is activated downstream of growth factor receptors. It is a key player in signaling pathways that regulate energy metabolism, proliferation, and cell survival (1). AKT1 was first described as the cellular homologue of the product of v-akt oncogene. Protein kinase B, also known as Akt was first described as the cellular homologue of the product of the v-akt oncogene. PKB comprise an N-terminal pleckstrin homology (PH) domain, a flexible hinge between the PH and the kinase domain, a catalytic (kinase) domain, and a C-terminal regulatory part (containing a hydrophobic motif, or HM). Activation of AKT depends upon the integrity of PH homology domain, which mediates its membrane translocation and the phosphorylation of Thr308 in the activation loop and Ser473 (2). AKT is a direct substrate of protein tyrosine kinase 6, that is not expressed in normal cells but induced in mammary cancer cells, phosphorylates AKT at Tyrosine residues Y315 and Y326. Protein kinase B is expressed in three isoforms (PKBalpha/AKT1, PKBbeta/AKT2 and PKBgamma/AKT3), AKT1 is expressed in multiple spliced variant forms. The AKT1 gene is catalytically yin active but selectively activated by PDGF and the activation is abrogated by mutations in the PH domain. The activation involves the IP3 kinase. Understanding the regulation of Akt has been of major interest for elucidating the control of normal cellular physiology as well as malignant transformation. The activation of Akt involves phosphatidylinositol 3-kinase-dependent membrane localization followed by activating phosphorylation of Thr-308 and Ser-473. Many of the activating signals for Akt involve the stimulation of receptor and non-receptor tyrosine kinases, and the most potent activator known is the tyrosine phosphatase inhibitor pervanadate, highlighting a possible role for tyrosine phosphorylation in the regulation of the enzyme (3). PKB is a 60 kDa protein expressed in majority of cells, mutations in this gene lead to proteus syndrome. . The AKT1-selective antibodies were generated against unique antigenic sequence form with in the C-terminal 50 amino acids. The sequence was conserved in rat, mouse, human and many other species, but was unique only toAKT1 and was not present in other protein kinases. The AKT antibodies were affinity purified over immobilized antigen based chromatography, and the affinity purified immunoglobulins are stabilized in antibody stabilization buffer.

General readings

Franke TF et al. PI3K: downstream AKTion blocks apoptosis. Cell 88:435-7 (1997). PubMed PMID: 9038334
Carpenter CL & Cantley LC Phosphoinositide kinases. Curr Opin Cell Biol 8:153-8 (1996). PubMed PMID: 8791418
Burgering BM & Coffer PJ Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376:599-602 (1995). PubMed PMID: 7637810
Franke TF et al. The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81:727-36 (1995). PubMed PMID: 7774014
5. Beffert, U., et al. (2002). Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. JBC. 277(51): 49958-49964.
6. Hsu, J., et al. (2001). The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9): 2853-2855.
7. Persad, S., et al. (2001). Regulation of protein kianse B/Akt-theronine-308 phosphorylation by integrin kinked kinase (ilk): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. JBC. 276(29): 27462-27469.
8. Nelson, J.M., & Fry, D.W. (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. JBC. 276(18): 14842-14847.
9. Genot, E.M., et al. (2000) The T-Cell receptor regulates Akt (Protein Kinase B)via a pathway involving Rac1 and phosphotidylinositide 3-kinase. Mol. Cell. Biol. 20(15): 5469-5478.
10. Datta, S.R., et al. (1997) Akt phosphoylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell. 91(2): 231-241.
11. del Peso, L., et al. (1997) Interleukin-2-induced phosphoylation of BAD through the protein kinase Akt. Science. 278(5338): 687-689.
12. Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kianse Akt. Science. 275(5300): 661-665.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT pSer473 antibody

Immunohistochemical analysis of AKT (pS473) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT pSer473 antibody

Immunohistochemical analysis of AKT (pS473) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT pSer473 antibody

Immunohistochemical analysis of AKT (pS473) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT pThr308 antibody

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, IP, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT pThr308 antibody

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, IP, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT pThr308 antibody

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, IP, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT pTyr315 antibody

Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT pTyr315 antibody

Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT pTyr315 antibody

Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Items 1 to 20 of 323 total

per page
Page:
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