AKT1 / PKB antibody

Principal name

AKT1 / PKB antibody

Alternative names for AKT1 / PKB antibody

Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase

SwissProt ID

P31749 (Human), P31750 (Mouse), P47196 (Rat), Q01314 (Bovin)

Gene ID

207 (AKT1), 11651 (Akt1), 24185

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), African clawed frog, Chimp (Chimpanzee), Rb (Rabbit), Mky (Monkey), Sh (Sheep), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot), ELISA (detection) (E(detection)), Flow Cytometry (F), EPair for Elisa (EPair), Luminex (LMNX)

Background of AKT1 / PKB antibody

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. AKT has been shown to play a role in such metabolic processes as glucose transport, glycogen synthesis, glycolysis, and protein synthesis. It had also been shown to promote cell survival by inhibiting apoptosis through its ability to phosphoylate and inactivate several targets, including Bad, Forkhead transcription factors, and caspase 9. Activity of AKT has been associated with the phosphorylation of two sites: T308, in the activation loop of the kinase, and S473, at the carboxyl terminus. Phosphorylation of both sites contributes to AKT activity, however phosphorylation of T308 has been shown to be absolutely essential for AKT activation.

General readings

Beffert, U., et al. (2002) Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. J. Biol. Chem. 277(51):49958-49964.
Hsu, J., et al. (2001) The AKT kinase is activated in multiple myeloma tumor cells. Blood 98(9):2853-2855.
Persad, S., et al. (2001) Regulation of protein kinase B/Akt-threonine-308 phosphorylation by integrin linked kinase (ILK): Critical roles for kinase activity and amino acids arginine-211 and threonine-343. J. Biol. Chem. 276(29):27462-27469.
Nelson, J.M. and D.W. Fry. (2001) Akt, MAPK (Erk1/2), and p38 act in concert to promote apoptosis in response to ErbB receptor family inhibition. J. Biol. Chem. 276(18):14842-14847.
Genot, E.M., et al. (2000) The T-Cell receptor regulates Akt (Protein Kinase B) via a pathway involving Rac1 and phosphotidylinositide 3-Kinase. Mol. Cell. Biol. 20(15):5469-5478.
Datta, S.R., et al. (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91(2):231-241
del Peso, L., et al. (1997) Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 278(5338):687-689
Dudek, H., et al. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt. Science 275(5300):661-665
Franke, T.F., et al. (1997) PI3K: downstream AKTion blocks apoptosis. Cell 88(4):435-437

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT pSer473 antibody

Immunohistochemical analysis of AKT (pS473) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT pSer473 antibody

Immunohistochemical analysis of AKT (pS473) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT pSer473 antibody

Immunohistochemical analysis of AKT (pS473) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT pThr308 antibody

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, IP, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT pThr308 antibody

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, IP, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT pThr308 antibody

Immunofluorescent analysis of AKT (pT308) staining in HL60 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, IP, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT pTyr315 antibody

Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT pTyr315 antibody

Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT pTyr315 antibody

Immunohistochemical analysis of AKT (pY315) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze ICC/IF, P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Por, Rt, Sh, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT antibody

Immunohistochemical analysis of AKT staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt, Sh, Ze IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Items 1 to 20 of 342 total

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