AKT1S1 / PRAS40 antibody

Principal name

AKT1S1 / PRAS40 antibody

Alternative names for AKT1S1 / PRAS40 antibody

Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

SwissProt ID

Q96B36 (Human), Q9D1F4 (Mouse)

Gene ID

84335 (AKT1S1), 67605 (Akt1s1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of AKT1S1 / PRAS40 antibody

Akt, also known as protein kinase B, is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, inhibition of apoptosis and transformation of cells. The proline-rich Akt substrate PRAS40, also designated AKT1S1, becomes phosphorylated by activated Akt on Ser or Thr residues in the motif RXRXX(S/T). Phosphorylated PRAS40 subsequently binds 14-3-3 in a sequence-specific manner, thereby inducing such changes as alteration of protein subcellular localization and regulation of intrinsic enzymatic activity. Studies also suggest that PRAS40 phosphorylation and its interaction with pAkt and 14-3-3 may play an important role in neuroprotection mediated by NGF in apoptotic neuronal cell death after cerebral ischemia. PRAS40 maps to human chromosome 19q13.33.

General readings

Fonseca,B.D., (2008) Biochem. J. 411 (1), 141-149

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT1S1 / PRAS40 antibody

  Rabbit polyclonal AKT1S1 Antibody (C-term)  
AKT1S1 Antibody (C-term) (Cat. #TA324543) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the AKT1S1 antibody detected the AKT1S1 protein (arrow). Rabbit IgG Purified Hu, Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AKT1S1 / PRAS40 antibody

  Rabbit Polyclonal Anti-AKT1S1 Antibody  
WB Suggested Anti-AKT1S1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

AKT1S1 / PRAS40 antibody

  Rabbit Polyclonal Anti-AKT1S1 Antibody  
Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT1S1 / PRAS40 antibody

Immunohistochemistry (IHC) analyzes of Akt1 S1 antibody (Cat.-No.: AP06491PU-N) in paraffin-embedded human breast tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

AKT1S1 / PRAS40 pThr246 antibody

Western blot analysis of extracts from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

AKT1S1 / PRAS40 pThr246 antibody

Western blot analysis of extracts from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

AKT1S1 / PRAS40 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt1 S1 (Ab-246) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €230.00
  Assay Biotech

AKT1S1 / PRAS40 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Akt1 S1 (Ab-246) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €330.00
  Assay Biotech

AKT1S1 / PRAS40 antibody

Human 293T; WB Suggested Anti-AKT1S1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate; AKT1S1 antibody - C-terminal region (ARP48061_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

AKT1S1 / PRAS40 pThr246 antibody

Western blot analysis of PRAS40 (pT246) expression in LO2 (A); Raw264.7 (B); NIH3T3 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

AKT1S1 / PRAS40 pThr246 antibody

Western blot analysis of PRAS40 (pT246) expression in LO2 (A); Raw264.7 (B); NIH3T3 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

AKT1S1 / PRAS40 pThr246 antibody

Western blot analysis of PRAS40 (pT246) expression in LO2 (A); Raw264.7 (B); NIH3T3 (C) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

AKT1S1 / PRAS40 antibody

Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

AKT1S1 / PRAS40 antibody

Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

AKT1S1 / PRAS40 antibody

Immunohistochemical analysis of PRAS40 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

CRISPR

Catalog No. Species Donor Vector  

AKT1S1 - human gene knockout kit via CRISPR

AKT1S1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AKT1S1 - human gene knockout kit via CRISPR

AKT1S1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AKT1S1 - human gene knockout kit via CRISPR

AKT1S1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AKT1S1 - human gene knockout kit via CRISPR

AKT1S1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Akt1s1 - mouse gene knockout kit via CRISPR

Akt1s1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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