AKT2 / PKB beta antibody

Principal name

AKT2 / PKB beta antibody

Alternative names for AKT2 / PKB beta antibody

RAC-PK-beta, Protein kinase Akt-2, Protein kinase B beta, RAC-beta serine/threonine-protein kinase

SwissProt ID

P31751 (Human), P47197 (Rat), Q38898, Q60823 (Mouse)

Gene ID

208 (AKT2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Mky (Monkey), Rhesus Mky (Rhesus Monkey), GP (Guinea Pig), Hst (Hamster), Rb (Rabbit), Sh (Sheep), Xen (Xenopus)

Available hosts

Mouse, Rabbit, Chicken, Goat, Rat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Dot blot (Dot), PLA/Proximity ligation assay (PLA), Flow Cytometry (F), Luminex (LMNX)

Background of AKT2 / PKB beta antibody

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis . This protein kise is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kise. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and ictivation of several targets, including Bad, forkhead transcription factors, c-Raf, and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kise/Akt sigling pathway. LY294002 is a specific PI3 kise inhibitor. Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and ictivation of GSK-3a and ß. Akt may also play a role in insulin stimulation of glucose transport. In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3ß-mediated phosphorylation and degradation of cyclin D1 and by negatively regulating the cyclin dependent kise inhibitors p27 Kip and p21 Waf1/CIP1. Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, Akt phosphorylates and ictivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT2 / PKB beta (N-term) antibody

Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat.#TA302204) in mouse liver tissue lysates (35ug/lane). AKT2(arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

Immunoprecipitation(IP) of AKT2 by using TrueMab monoclonal anti-AKT2 antibodies (Negative control: IP without adding anti-AKT2 antibody.). For each experiment, 500ul of DDK tagged AKT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-AKT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. Mouse IgG1 OTI8D9 Purified Hu F, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT2 / PKB beta antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AKT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500814) Mouse IgG1 OTI4H7 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

AKT2 / PKB beta antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AKT2 rabbit polyclonal antibody. (TA590729) Rabbit IgG Purified Hu E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

AKT2 monoclonal antibody (M02), clone 1B4. Western Blot analysis of AKT2 expression in NIH/3T3(Cat # L018V1 ). Mouse IgG1 1B4 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT2 / PKB beta antibody

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1D9 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT2 / PKB beta antibody

Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG1 1F3 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT2 / PKB beta antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AKT2 (phospho S474) polyclonal antibody ( Cat # PAB8183 ). Rabbit Hu, Ms, Rt P, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

AKT2 / PKB beta antibody

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta (C-term) antibody

(0.3ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

Western Blot of Rat anti-AKT2 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT2 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rat secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT2. Other band(s): none. Rat 16G11.A7 Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and breast cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using AKT2 antibody at dilution 1/70, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta (C-term) antibody

Western blot analysis of AKT2 (arrow) using AKT2 Antibody (C-term) (Cat.#TA324897). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

Western blot analysis of Akt2 expression in TNF-a treated A2780 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta specific antibody

Western blot analysis of AKT2 (PKBbeta) in cell line mix using a 1:1000 dilution of the antibody Rabbit IgG Purified Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen E, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

Western blot analysis of extracts of various cell lines, using AKT2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT2 / PKB beta antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2. Mouse IgG2a OTI1A2 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT2 / PKB beta antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT2 (RC217733, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT2. Mouse IgG1 OTI8G8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT2 / PKB beta antibody

Immunoprecipitation(IP) of AKT2 by using TrueMab monoclonal anti-AKT2 antibodies (Negative control: IP without adding anti-AKT2 antibody.). For each experiment, 500ul of DDK tagged AKT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-AKT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody. Mouse IgG1 OTI4G1 Purified Hu IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT2 / PKB beta antibody

AKT2 ELISA with 1A2 Capture (TA600009) and 8D9 Detection (TA700009) Antibodies. Substrate used: Recombinant Human AKT2 (TP317733) Mouse IgG2a OTI1A2 Purified Hu E, LMNX
0.1 mg / €325.00
  OriGene Technologies, Inc.

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