AKT3 / PKB gamma antibody

Principal name

AKT3 / PKB gamma antibody

Alternative names for AKT3 / PKB gamma antibody

RAC-PK-gamma, Protein kinase B gamma, PKBG, STK-2, RAC-gamma serine/threonine-protein kinase

SwissProt ID

Q63484 (Rat), Q9WUA6 (Mouse), Q9Y243 (Human)

Gene ID

10000 (AKT3)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Chk (Chicken), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Ze (Zebrafish), Tr (Turkey), Bear

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot), Whole mount Immunohistochemistry (IHC-wm)

Background of AKT3 / PKB gamma antibody

Akt3 (also designated protein kinase B gamma or v-akt murine thymoma viral oncogene homolog 3), with 479-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt2. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. Akt3 is not required for the maintenance of normal carbohydrate metabolism but is essential for the attainment of normal organ size. Identifying Akt3 as a selective target in melanoma cells also provides new therapeutic opportunities for patients in the advanced stages of this disease.

General readings

Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clin Cancer Res. 2001 Aug;7(8):2475-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT3 / PKB gamma antibody

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500001) Mouse IgG3 OTI9B2 Purified Hu, Ms F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

AKT3 / PKB gamma antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-AKT3 (TA500080) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min). Mouse IgG3 OTI9H8 Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT3 / PKB gamma antibody

AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 3F3 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT3 / PKB gamma antibody

AKT3 monoclonal antibody (M04), clone 4C1. Western Blot analysis of AKT3 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 4C1 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT3 / PKB gamma antibody

AKT3 monoclonal antibody (M06), clone 2H4. Western Blot analysis of AKT3 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 2H4 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT3 / PKB gamma antibody

AKT3 monoclonal antibody (M07), clone 6F12. Western Blot analysis of AKT3 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 6F12 Purified Hu, Ms E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT3 / PKB gamma antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCZ and AKT3. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-AKT3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 6E11 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

AKT3 / PKB gamma antibody

TA302640 (1µg/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
20 µg / €200.00
  Acris Antibodies GmbH

AKT3 / PKB gamma antibody

Western blot analysis of lysate from Jurkat cell line,using AKT3 Antibody (Q103)(Cat. #TA300042).TA300042 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Rabbit Ig Purified Hu, Mky, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

Western blot analysis of AKT3 (arrow) using rabbit polyclonal AKT3 Antibody (S472) (Cat.#TA302230). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT3 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

TA302640 (1µg/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using AKT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using AKT3 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

Western Blot: AKT3 Antibody (66C1247) [TA336674] - Analysis for AKT3 using TA336674 at 2 ug/ml dilution against 30 ug/lane of human kidney lysate. Mouse IgG1 66C1247 Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma (C-term) antibody

Anti – AKT3 (DB 184)Western blot analysis of DB 184 sensitivity using recombinant proteins AKT1, AKT2 and AKT3: lane 1 – 0.2 μg of human His- AKT1; lane 2 -0.2 μg of human GST-AKT2; lane 3 – 1 μg of human GST-AKT3, lane 4 – 0.5 μg of human GST-AKT3, lane 5 – 0.2 μg of human GST-AKT3 loaded. Rabbit IgG E18-H Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 ml / €580.00
  DB Biotech, spol. s r.o.

Sample sizes available

AKT3 / PKB gamma (C-term) antibody

Anti – AKT3 (DB 184)Western blot analysis of DB 184 sensitivity using recombinant proteins AKT1, AKT2 and AKT3: lane 1 – 0.2 μg of human His- AKT1; lane 2 -0.2 μg of human GST-AKT2; lane 3 – 1 μg of human GST-AKT3, lane 4 – 0.5 μg of human GST-AKT3, lane 5 – 0.2 μg of human GST-AKT3 loaded. Rabbit IgG E18-H Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
50 µl / €380.00
  DB Biotech, spol. s r.o.

Sample sizes available

AKT3 / PKB gamma (119-136) antibody

Formalin-Fixed, Paraffin-Embedded Human Brain tissue stained with AKT3 Antibody Cat.-No AM06001PU-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 66C1247.1 Purified Bear, Bov, Bt, Can, Chk, Elephant, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr, Ze P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AKT3 / PKB gamma antibody

Western blot analysis using Akt3 antibody Cat.-No AM06129SU-N against truncated Akt3 recombinant protein (Lane 1) and human ovary carcinoma tissue lysate (Lane 2). Mouse IgG 3B12F3F10 Ascites Hu E, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

AKT3 / PKB gamma antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AKT3 / PKB gamma pSer472 antibody

Dot blot analysis of anti-AKT3-pSer472 Phospho-specific Pab (CAt#AP12884PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

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