AKT3 / PKB gamma antibody

Principal name

AKT3 / PKB gamma antibody

Alternative names for AKT3 / PKB gamma antibody

RAC-PK-gamma, Protein kinase B gamma, PKBG, STK-2, RAC-gamma serine/threonine-protein kinase

SwissProt ID

Q63484 (Rat), Q9WUA6 (Mouse), Q9Y243 (Human)

Gene ID

10000 (AKT3), 23797 (Akt3), 29414

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Hst (Hamster), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Ze (Zebrafish), Tr (Turkey), Bear, Eq (Equine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP), Dot blot (Dot), Flow Cytometry (F)

Background of AKT3 / PKB gamma antibody

Kit Component:
- KN301102G1, Akt3 gRNA vector 1 in pCas-Guide vector
- KN301102G2, Akt3 gRNA vector 2 in pCas-Guide vector
- KN301102D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clin Cancer Res. 2001 Aug;7(8):2475-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

AKT3 / PKB gamma antibody

  AKT3 mouse monoclonal antibody, clone OTI9B2 (formerly 9B2)  
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500001) Mouse IgG3 OTI9B2 Purified Hu, Ms F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

AKT3 / PKB gamma antibody

  anti-AKT3 mouse monoclonal antibody, clone OTI9H8 (formerly 9H8)  
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-AKT3 (TA500080) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min). Mouse IgG3 OTI9H8 Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

AKT3 / PKB gamma antibody

  AKT3 Antibody (Center ) Rabbit Polyclonal Antibody  
Western blot analysis of lysate from Jurkat cell line,using AKT3 Antibody (Q103)(Cat. #TA300042).TA300042 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Rabbit Ig Purified Hu, Mky, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

  Rabbit Polyclonal Antibody against AKT3 (S472)  
Western blot analysis of AKT3 (arrow) using rabbit polyclonal AKT3 Antibody (S472) (Cat.#TA302230). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT3 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

  Goat Polyclonal Antibody against AKT3  
TA302640 (1µg/ml) staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

  Anti-AKT3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human gastric and lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human gastric cancer tissue using AKT3 antibody at dilution 1/30, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

  Anti-AKT3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human brain and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using AKT3 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma antibody

  Mouse Monoclonal Akt3 Antibody (66C1247)  
Western Blot: AKT3 Antibody (66C1247) [TA336674] - Analysis for AKT3 using TA336674 at 2 ug/ml dilution against 30 ug/lane of human kidney lysate. Mouse IgG1 66C1247 Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

AKT3 / PKB gamma (C-term) antibody

Anti – AKT3 (DB 184)Western blot analysis of DB 184 sensitivity using recombinant proteins AKT1, AKT2 and AKT3: lane 1 – 0.2 μg of human His- AKT1; lane 2 -0.2 μg of human GST-AKT2; lane 3 – 1 μg of human GST-AKT3, lane 4 – 0.5 μg of human GST-AKT3, lane 5 – 0.2 μg of human GST-AKT3 loaded. Rabbit IgG E18-H Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
0.1 ml / €580.00
  DB Biotech, spol. s r.o.

Sample sizes available

AKT3 / PKB gamma (C-term) antibody

Anti – AKT3 (DB 184)Western blot analysis of DB 184 sensitivity using recombinant proteins AKT1, AKT2 and AKT3: lane 1 – 0.2 μg of human His- AKT1; lane 2 -0.2 μg of human GST-AKT2; lane 3 – 1 μg of human GST-AKT3, lane 4 – 0.5 μg of human GST-AKT3, lane 5 – 0.2 μg of human GST-AKT3 loaded. Rabbit IgG E18-H Aff - Purified Hu, Ms, Rt E, ICC/IF, IP, WB
50 µl / €380.00
  DB Biotech, spol. s r.o.

Sample sizes available

AKT3 / PKB gamma (119-136) antibody

Formalin-Fixed, Paraffin-Embedded Human Brain tissue stained with AKT3 Antibody Cat.-No AM06001PU-N at 10 µg/ml after heat-induced antigen retrieval. Mouse IgG1 66C1247.1 Purified Bear, Bov, Bt, Can, Chk, Elephant, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr, Ze P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AKT3 / PKB gamma antibody

Western blot analysis using Akt3 antibody Cat.-No AM06129SU-N against truncated Akt3 recombinant protein (Lane 1) and human ovary carcinoma tissue lysate (Lane 2). Mouse IgG 3B12F3F10 Ascites Hu E, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

AKT3 / PKB gamma pSer472 antibody

Dot blot analysis of anti-AKT3-pSer472 Phospho-specific Pab (CAt#AP12884PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

AKT3 / PKB gamma (119-133) antibody

Immunohistochemistry Image: Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr, Ze E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

AKT3 / PKB gamma (119-136) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

AKT3 - human gene knockout kit via CRISPR

AKT3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

AKT3 - human gene knockout kit via CRISPR

AKT3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

AKT3 - human gene knockout kit via CRISPR

AKT3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

AKT3 - human gene knockout kit via CRISPR

AKT3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Akt3 - mouse gene knockout kit via CRISPR

Akt3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

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