ALAS1 antibody

Principal name

ALAS1 antibody

Alternative names for ALAS1 antibody

ALAS3, ALASH, Delta-ALA synthetase, 5-aminolevulinate synthase nonspecific mitochondrial, 5-aminolevulinic acid synthase, Delta-aminolevulinate synthase

SwissProt ID

A6QLI6 (Bovin), P13195 (Rat), P13196 (Human)

Gene ID

211 (ALAS1), 212 (ALAS2)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Xen (Xenopus), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ALAS1 antibody

Official Gene Symbol: ALAS1 Gene ID: 211(human) Gene Map Locus: 3p21.1(human) ALAS-1 (EC 2.3.1.37) is a nuclear-encoded mitochondrial matrix enzyme catalyzing the condensation of glycine with succinyl-CoA to form delta-amino levulinate, CO2 and CoA. It regulates the first and rate-limiting step of heme biosynthetic pathway. It is one of the two isoforms of ALAS and is a pyridoxal-5' phosphate dependant housekeeping enzyme. It is ubiquitously expressed and is responsible of providing heme for cytochromes and other hemoproteins. ALAS1 in liver undergoes negative feedback regulation by heme. Transcription of ALAS1 gene is stimulated by cAMP and respiratory uncoupling whereas Phorbol esters and insulin repress ALAS1 gene expression. ALAS1 is suggested to be involved in reciprocal regulation of Heme biosynthesis and circadian clock suggesting a potential target for treatment of circadian disorders. ALAS1 gene expression is down regulated in Acute Liver failure resulting in altered heme metabolism and liver function.

General readings

Jung,M., (2007) Urologe A 46 (9), 1083-1084

17 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALAS1 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast and thyroid cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ALAS1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALAS1 antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using ALAS1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALAS1 antibody

WB Suggested Anti-ALAS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Lung Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ALAS1 antibody

Western Blot: Alas1 Antibody [TA336353] - analysis of HepG2 cell lysate using ALAS1 antibody at1 ug/ml. Rabbit Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALAS1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALAS1 (RC219938, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALAS1.(1:2000) Mouse IgG1 OTI1C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALAS1 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALAS1 (RC219938, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALAS1.(1:2000) Mouse IgG1 OTI5D5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALAS1 (480-530) antibody

Skin: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ALAS1 antibody

ALAS1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

ALAS1 antibody

ALAS1 monoclonal antibody (M01), clone 3G10 Western Blot analysis of ALAS1 expression in JAR ( Cat # L003V1 ). Mouse IgG2a 3G10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ALAS1 antibody

ALAS1 monoclonal antibody (M03), clone 2D5. Western Blot analysis of ALAS1 expression in COLO 320 HSR ( Cat # L020V1 ). Mouse IgG2a 2D5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ALAS1 antibody

Western blot analysis of ALAS1 in HepG2 cell lysate. Using ALAS1 polyclonal antibody ( Cat # PAB0349 ) at 1 µg/ml. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ALAS1 antibody

Human Lung; WB Suggested Anti-ALAS1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Lung; ALAS1 antibody - N-terminal region (ARP54346_P050) in Human Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ALAS1 antibody

Western Blot: ALAS1 Antibody [NBP1-54668] - This Anti-ALAS1 antibody was used in Western Blot of Fetal Lung tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Xen, Ze WB
50 µg / €440.00
  Novus Biologicals Inc.

ALAS1 antibody

Immunohistochemistry-Paraffin: Alas1 Antibody [NBP1-91656] - Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ALAS1 (full length, N-term HIS tag, transcript variant 2)

ALAS1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

ALAS1

ALAS1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ALAS1

ALAS1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

17 Item(s)

per page
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