Alcohol dehydrogenase 5 / ADH5 antibody

Principal name

Alcohol dehydrogenase 5 / ADH5 antibody

Alternative names for Alcohol dehydrogenase 5 / ADH5 antibody

Alcohol dehydrogenase class 3 chi chain, S-(hydroxymethyl)glutathione dehydrogenase, Glutathione-dependent formaldehyde dehydrogenase

SwissProt ID

P11766 (Human), P12711 (Rat), P28474 (Mouse), Q3ZC42 (Bovin)

Gene ID

128 (ADH5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Por (Porcine), Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P)

Background of Alcohol dehydrogenase 5 / ADH5 antibody

The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including retinol, hydroxysteroids, ethanol, aliphatic alcohols and lipid peroxidation products. ADH5 (alcohol dehydrogenase 5 (class III)), also known as FDH (formaldehyde dehydrogenase), ADHX, ADH-3 or GSNOR, is a 374 amino acid cytoplasmic protein that belongs to the class III subfamily of alcohol dehydrogenases. Expressed ubiquitously, ADH5 uses iron as a cofactor to catalytically oxidize both long-chain primary alcohols and S-hydroxymethyl-glutathione, a product formed spontaneously between formaldehyde and glutathione. ADH5 exists as a homodimer and, via its ability to oxidize S-hydroxymethyl-glutathione and, thus, eliminate formaldehyde, functions as an important component of cellular metabolism. Genetic variations in the gene encoding ADH5 may affect drug and alcohol dependence in humans.

General readings

Adinolfi A, Adinolfi M, Hopkinson DA. Immunological and biochemical characterization of the human alcohol dehydrogenase chi-ADH isozyme. Ann Hum Genet. 1984 Jan;48(Pt 1):1-10. PMID: 6424546

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alcohol dehydrogenase 5 / ADH5 antibody

TA305835 (0.5µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alcohol dehydrogenase 5 / ADH5 antibody

Predicted band size: 40 kDa . Positive control: Kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alcohol dehydrogenase 5 / ADH5 antibody

WB Suggested Anti-ADH5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human kidney Rabbit IgG Purified Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alcohol dehydrogenase 5 / ADH5 antibody

Western blot analysis of ADH5 Antibody (Center) (AP17870PU-N) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alcohol dehydrogenase 5 / ADH5 antibody

ADH5 polyclonal antibody (A01), Lot # DUM6060320QCS1. Western Blot analysis of ADH5 expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5 antibody

Western Blot analysis of ADH5 expression in transfected 293T cell line (H00000128-T01) by ADH5 MaxPab polyclonal antibody.

Lane 1: ADH5 transfected lysate(41.14 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5 antibody

ADH5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ADH5 expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5 antibody

Alcohol dehydrogenase 5 / ADH5 Mouse IgG2a 12F7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5 antibody

Alcohol dehydrogenase 5 / ADH5 Mouse IgG2b 2D11 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5 antibody

Alcohol dehydrogenase 5 / ADH5 Goat Hu, Ms, Rt E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5 antibody

Alcohol dehydrogenase 5 / ADH5 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Alcohol dehydrogenase 5 / ADH5 antibody

Alcohol dehydrogenase 5 / ADH5 Goat IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Alcohol dehydrogenase 5 / ADH5 antibody

Western Blot: alcohol dehydrogenase 5 Antibody [NBP1-56447] - This Anti-ADH5 antibody was used in Western Blot of Fetal Kidney    tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Alcohol dehydrogenase 5 / ADH5

Alcohol dehydrogenase 5 / ADH5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Alcohol dehydrogenase 5 / ADH5 (1-374, His-tag)

Alcohol dehydrogenase 5 / ADH5 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Alcohol dehydrogenase 5 / ADH5 (1-374, His-tag)

Alcohol dehydrogenase 5 / ADH5 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Alcohol dehydrogenase 5 / ADH5

Alcohol dehydrogenase 5 / ADH5 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5

Alcohol dehydrogenase 5 / ADH5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5

Alcohol dehydrogenase 5 / ADH5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Alcohol dehydrogenase 5 / ADH5

Alcohol dehydrogenase 5 / ADH5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

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