ALDH1 antibody

Principal name

ALDH1 antibody

Alternative names for ALDH1 antibody

Retinal dehydrogenase 1, RALDH1, ALDH1A1, ALDC, PUMB1, Aldehyde dehydrogenase cytosolic, ALHDII, ALDH-E1, Aldehyde dehydrogenase family 1 member A1

SwissProt ID

P00352 (Human), P24549 (Mouse), P27463 (Chick), P48644 (Bovin), P51647 (Rat)

Gene ID

216 (ALDH1A1), 11668 (Aldh1a1), 24188

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Chk (Chicken), Eq (Equine), Mky (Monkey), Op (Opossum), Prim (Primate), Sh (Sheep), Xen (Xenopus), L (Lizard)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ALDH1 antibody

ALDH1A1 is an aldehyde dehydrogenase able to oxidize a wide variety of aliphatic aldehydes (retinaldehyde, acetaldehyde, etc.) to the corresponding carboxylic acids (retinoic acid, acetic acid, etc.). ALDH1A1 (also known as RALDH1, ALDH1, or AHD2) is highly expressed in the dorsal retina, ventral midbrain (dopaminergic neurons), and hematopoietic stem cells. ALDH1A1 is involved in retinoic acid (RA) synthesis during vertebrate embryogenesis. ALDH1A1 is first detected at E9.0-E10.5 in cranial tissues (ventral mesencephalon, dorsal retina, thymic primordia, optic vesicles) and in the mesonephros. ALDH1A1 is also of interest in Parkinson's Disease (PD) being expressed in the the A9 dopaminergic (DA) neuronal group projecting to the dorsal striatum; this is the most vulnerable site in PD (Chung et al, 2005). ALDH1A1 protein is a known mesencephalic dopaminergic marker. ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid .ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea .ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development .The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALDH1 (C-term) antibody

  Goat Polyclonal Antibody against ALDH1A1 (C Terminus)  
TA302641 staining (0.03µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALDH1 (476-488) antibody

Formalin-Fixed Paraffin-Embedded Human Testis stained with ALDH1A1 antibody Cat.-No AP08840PU-N  at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Goat IgG Aff - Purified Bov, Bt, Can, Chk, Eq, Hu, L, Mky, Ms, Op, Prim, Rt, Sh, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ALDH1 antibody

  Mouse monoclonal ALDH1A1 Antibody  
ALDH1A1 Monoclonal Antibody(Cat. #TA324334) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow). Mouse IgG1 152CT1.2.2 Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ALDH1 (1-502) antibody

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1G6 Purified Hu E, IP, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

ALDH1 antibody

  Rabbit Polyclonal Antibody against ALDH1A1 (Center)  
Western blot analysis of ALDH1A1 (arrow) using rabbit polyclonal ALDH1A1 Antibody (Center) (Cat# TA302024). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu F, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALDH1 antibody

  Goat Polyclonal Antibody against ALDH1A1 (Internal)  
TA302409 (1µg/ml) staining of Human Liver Lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALDH1 antibody

  Anti-ALDH1A1/ALDH1A2/ALDH1A3 Rabbit Polyclonal Antibody  
Predicted band size: 57 kDa. Positive control: Human liver cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/450 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALDH1 antibody

  Anti-ALDH1A1/ALDH1A2/ALDH1A3 Rabbit Polyclonal Antibody  
Predicted band size: 57 kDa. Positive control: Mouse liver tissue lysate . Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/400 dilution Secondary antibody: Donkey anti Rabbit IgG - H&L (HRP) at 1/3000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALDH1 antibody

  Rabbit polyclonal ALDH1A1 Antibody  
Western blot analysis of lysates from HepG2 cell line and human lung tissue lysate (from left to right),  using ALDH1A1 Antibody(Cat.  #TA324559).  TA324559 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.  Lysates at 35ug per lane. Rabbit IgG Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ALDH1 antibody

  Rabbit anti-ALDH1A1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALDH1 antibody

Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and lung cancer (right) using ALDH1A1 antibody Cat.-No AM06513SU-N with DAB staining. Mouse IgG1 5A11 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Aldh1a1 - mouse gene knockout kit via CRISPR

Aldh1a1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Aldh1a1 - mouse gene knockout kit via CRISPR

Aldh1a1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Aldh1a1 - mouse gene knockout kit via CRISPR

Aldh1a1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Aldh1a1 - mouse gene knockout kit via CRISPR

Aldh1a1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ALDH1

  Recombinant protein of human aldehyde dehydrogenase 1 family, member A1 (ALDH1A1)  
ALDH1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ALDH1 (1-501)

ALDH1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

ALDH1 (1-501)

ALDH1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Aldh1a1 (GFP-tagged) - Mouse aldehyde dehydrogenase family 1, subfamily A1 (Aldh1a1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Aldh1a1 (GFP-tagged) - Mouse aldehyde dehydrogenase family 1, subfamily A1 (Aldh1a1), 200 uL, >10^7 TU/mL  
200 µl / €1,120.00
  OriGene Technologies, Inc.

Lenti ORF particles, Aldh1a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 1, subfamily A1 (Aldh1a1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Aldh1a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 1, subfamily A1 (Aldh1a1), 200 uL, >10^7 TU/mL  
200 µl / €1,000.00
  OriGene Technologies, Inc.

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