Aldolase A / ALDOA antibody

Principal name

Aldolase A / ALDOA antibody

Alternative names for Aldolase A / ALDOA antibody

ALDA, NY-LU-1, Muscle-type aldolase, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1

SwissProt ID

P00883 (Rabit), P04075 (Human), P05064 (Mouse), P05065 (Rat)

Gene ID

100009055 (ALDOA), 226

Available reactivities

Hu (Human), Rb (Rabbit), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Can (Canine)

Available hosts

Goat, Mouse, Rabbit, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R)

Background of Aldolase A / ALDOA antibody

ALDOA is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

General readings

Pfleiderer G., et al. (1975) Beitr Pathol. 156(3):266-79. Kishi H., et al. (1987) Proc Natl Acad USA. 84(23):8623-7.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aldolase A / ALDOA antibody

Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Goat IgG Purified Hu, Rb E, ICC/IF, IP, P, WB
50 µl / €530.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Western blot analysis of Hela cell and HepG2 cell lysate using ALDOA antibody. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA antibody

Western blot analysis of extracts from A549 cells, HT-29 cells and HeLa cells, using ALDOA antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA antibody

Predicted band size: 39 kDa . Positive control: A549 and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA antibody

Predicted band size: 39 kDa . Positive control: A549 and hela cell lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA (N-term) antibody

Western blot analysis of lysates from 293,RD,mouse NIH/3T3,rat L6 cell line (from left to right),using ALDOA Antibody (N-term)(Cat. #TA324726).TA324726 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Rabbit Ig Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA antibody

Anti-ALDOA antibody (1µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA (86-96) antibody

TA329071 (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Aldolase A / ALDOA antibody

Formalin-Fixed, Paraffin-Embedded  Human Human Kidney, Tubules stained with Aldolase A / ALDOA Antibvody Cat.-No AM31917PU-N (Clone 2E6) at 5 µg/ml after heat-induced antigen retrieval. Mouse 2E6 Purified Hu, Ms E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Western blot (WB) analysis of Aldolase A antibody (Cat.-No.: AP06656PU-N) at 1/500 dilution Lane1:HEK293T whole cell lysate Lane2:HepG2 whole cell lysate Lane3:NIH-3T3 whole cell lysate Lane4:PC12 whole cell lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Aldolase A / ALDOA (C-term) antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody (C-term) (Cat.#AP12350PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Aldolase A / ALDOA Sheep IgG Azide Free Rb Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Aldolase A / ALDOA Sheep IgG Biotin Rb Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Aldolase A / ALDOA Sheep IgG Purified Rb Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Immunoprecipitation and Western blot. 300 µl aliquots of whole anti-aldolase antiserum (Cat.-No. R1054) were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 µl 0.1 N NaOH.  90 µl of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.  Gel was stained, destained and imaged using standard protocols.  Precipitation of aldolase was confirmed by comparison of increasing amounts of antigen with the control protein by SDS PAGE and observation of a 40-45 kD MW band corresponding to Aldolase.  Additional higher and lower molecular weight bands correspond to serum proteins. Goat Serum Hu, Rb E, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Aldolase A / ALDOA Goat Biotin Hu, Rb E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

IgG purified antibody to rabbit muscle aldolase (R1054, R1054P and R1054HRP) was used at a 1/1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. Goat HRP Hu, Rb E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

IgG purified antibody to rabbit muscle aldolase (R1054, R1054P and R1054HRP) was used at a 1/1000 dilution to detect human aldolase by western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase. Goat Purified Hu, Rb E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Aldolase A / ALDOA antibody

Aldolase A / ALDOA Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Aldolase A / ALDOA antibody

Aldolase A / ALDOA Mouse Hu WB
50 µg / €370.00
  Abnova Taiwan Corp.

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