Aldolase B / ALDOB antibody

Principal name

Aldolase B / ALDOB antibody

Alternative names for Aldolase B / ALDOB antibody

Liver-type aldolase, Fructose-bisphosphate aldolase B

SwissProt ID

P00884 (Rat), P05062 (Human), P07341 (Chick), P52210 (Sheep), P79226 (Rabit), Q3T0S5 (Bovin), Q5RFA6, Q8JH71, Q91Y97 (Mouse)

Gene ID

229 (ALDOB), 230163 (Aldob)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit), Mky (Monkey), Por (Porcine), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Aldolase B / ALDOB antibody

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related \'housekeeping\' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13 ) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related \'housekeeping\' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Caffe A R., et al. (1994) J Comp Neurol. 348:291-297.Dehnes Y., et al. (1998) J Neurosci. 18:3606-3619.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aldolase B / ALDOB antibody

Immunohistochemistry analyzes of Aldolase B antibody (Cat.-No.: AP01402PU-N) in paraffin-embedded Human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Aldolase B / ALDOB antibody

  ALDOB mouse monoclonal antibody, clone OTI3C6 (formerly 3C6)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDOB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502908) Mouse IgG2b OTI3C6 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB antibody

  ALDOB mouse monoclonal antibody, clone OTI3E10 (formerly 3E10)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDOB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502909) Mouse IgG1 OTI3E10 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB antibody

  Rabbit Polyclonal Aldolase B Antibody  
Immunohistochemical staining of human kidney shows strong positivity in proximal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

  Rabbit polyclonal anti-ALDOB antibody  
Western blot analysis of extracts from MCF-7 cells and HUVEC cells, using ALDOB antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

  Anti-ALDOB Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

  Anti-ALDOB Rabbit Polyclonal Antibody  
Predicted band size: 39 kDa . Positive control: Human fetal liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/260 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

  ALDOB mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDOB (RC220062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDOB. Mouse IgG1 OTI2A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB antibody

  ALDOB mouse monoclonal antibody, clone OTI 5D1 (formerly 5D1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDOB (RC220062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDOB. Mouse IgG1 OTI 5D1 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB (111-160) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aldolase B / ALDOB antibody

Western blot analysis of extracts from MCF-7/HuvEc cells, using ALDOB Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

Aldolase B / ALDOB antibody

Western blot analysis of extracts from MCF-7/HuvEc cells, using ALDOB Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ALDOB - human gene knockout kit via CRISPR

ALDOB - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ALDOB - human gene knockout kit via CRISPR

ALDOB - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ALDOB - human gene knockout kit via CRISPR

ALDOB - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ALDOB - human gene knockout kit via CRISPR

ALDOB - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Aldob - mouse gene knockout kit via CRISPR

Aldob - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Aldob - mouse gene knockout kit via CRISPR

Aldob - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Aldob - mouse gene knockout kit via CRISPR

Aldob - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Aldob - mouse gene knockout kit via CRISPR

Aldob - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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