Aldolase B / ALDOB antibody

Principal name

Aldolase B / ALDOB antibody

Alternative names for Aldolase B / ALDOB antibody

Liver-type aldolase, Fructose-bisphosphate aldolase B

SwissProt ID

P00884 (Rat), P05062 (Human), P07341 (Chick), P52210 (Sheep), P79226 (Rabit), Q3T0S5 (Bovin), Q5RFA6, Q8JH71, Q91Y97 (Mouse)

Gene ID

229 (ALDOB), 321664 (aldob)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit), Ze (Zebrafish), Mky (Monkey), Por (Porcine), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Aldolase B / ALDOB antibody

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related \'housekeeping\' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13 ) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related \'housekeeping\' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Caffe A R., et al. (1994) J Comp Neurol. 348:291-297.Dehnes Y., et al. (1998) J Neurosci. 18:3606-3619.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Aldolase B / ALDOB antibody

Immunohistochemistry analyzes of Aldolase B antibody (Cat.-No.: AP01402PU-N) in paraffin-embedded Human brain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Aldolase B / ALDOB antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDOB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502908) Mouse IgG2b OTI3C6 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDOB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502909) Mouse IgG1 OTI3E10 Purified Hu F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB antibody

Immunohistochemical staining of human kidney shows strong positivity in proximal tubules.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

Western blot analysis of extracts from MCF-7 cells and HUVEC cells, using ALDOB antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

Predicted band size: 39 kDa . Positive control: Mouse liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

Predicted band size: 39 kDa . Positive control: Human fetal liver tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/260 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Aldolase B / ALDOB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDOB (RC220062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDOB. Mouse IgG1 OTI2A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDOB (RC220062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDOB. Mouse IgG1 OTI 5D1 Purified Hu F, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Aldolase B / ALDOB (111-160) antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Aldolase B / ALDOB antibody

ALDOB polyclonal antibody (A01). Western Blot analysis of ALDOB expression in human ovary cancer. Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Aldolase B / ALDOB antibody

ALDOB MaxPab polyclonal antibody. Western Blot analysis of ALDOB expression in human liver. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Aldolase B / ALDOB antibody

Aldolase B / ALDOB Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Aldolase B / ALDOB antibody

Western Blot analysis of ALDOB expression in transfected 293T cell line (H00000229-T01) by ALDOB MaxPab polyclonal antibody.

Lane 1: ALDOB transfected lysate(39.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Aldolase B / ALDOB antibody

Detection limit for recombinant GST tagged ALDOB is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 3B3 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Aldolase B / ALDOB antibody

Western blot analysis of extracts from MCF-7/HuvEc cells, using ALDOB Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Aldolase B / ALDOB antibody

Western blot analysis of extracts from MCF-7/HuvEc cells, using ALDOB Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Aldolase B / ALDOB antibody

Human Liver; WB Suggested Anti-ALDOB Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; ALDOB antibody - middle region (ARP41365_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Aldolase B / ALDOB antibody

Immunohistochemical analysis of ALDOB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rb, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Aldolase B / ALDOB antibody

Immunohistochemical analysis of ALDOB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rb, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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