ALIX / PDCD6IP antibody

Principal name

ALIX / PDCD6IP antibody

Alternative names for ALIX / PDCD6IP antibody

PDCD6-interacting protein, ALG-2-interacting protein 1, Hp95, KIAA1375, Programmed cell death 6-interacting protein

SwissProt ID

Q8WUM4 (Human), Q9QZA2 (Rat), Q9WU78 (Mouse)

Gene ID

10015 (PDCD6IP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Frozen Sections (C)

Background of ALIX / PDCD6IP antibody

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation.

General readings

Morita E., et al. (2007) EMBO J. 26(19):4215-27.
Katoh K., et al. (2004) Arch Biochem Biophys. 421(1):159-65.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALIX / PDCD6IP antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDCD6IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507312) Mouse IgG2a OTI1A4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALIX / PDCD6IP antibody

PDCD6IP MaxPab rabbit polyclonal antibody. Western Blot analysis of PDCD6IP expression in mouse spleen. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP antibody

NIH3T3 whole cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Alix antibody (clone 3A9). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluninescence detection. Mouse IgG1 3A9 Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALIX / PDCD6IP antibody

Western blot analysis of HeLa whole cell lysate probed with Mouse Anti-ALIX (AM01318PU-N) F(ab')2 Rabbit Anti-Mouse IgG:HRP (SP1012HRP). Mouse IgG1 3A9 Purified Hu, Ms, Rt, Xen E, ICC/IF, IP, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

ALIX / PDCD6IP antibody

Western Blot: ALIX antibody (8E8) staining of 10 µg (lane 1) and 100 µg (lane 2) of recombinant GST-ALIX Mouse IgG1 8E8 Purified Hu C, E, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

ALIX / PDCD6IP antibody

BHK cell line transfected with Alix gene and stained with ALIX antibody Cat.-No AP54754SU-N Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

ALIX / PDCD6IP antibody

PDCD6IP MaxPab polyclonal antibody. Western Blot analysis of PDCD6IP expression in human stomach. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP antibody

ALIX / PDCD6IP Rabbit Serum Hu, Ms E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP antibody

Immunofluorescence of monoclonal antibody to PDCD6IP on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 3C4 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP antibody

Western Blot: PDC6IP Antibody (3A9) [NB100-65678] - Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (STAR13B) Mouse IgG1 3A9 Purified Hu, Ms, Rt, Xen E, ICC/IF, IP, P, WB
0.1 mg / €500.00
  Novus Biologicals Inc.

ALIX / PDCD6IP antibody

Western Blot: Alix Antibody [NBP1-49700]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Affinity purified rabbit anti-ALIX antibody used for IP at 3 mcg/mg lysate.  ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49701, which recognizes a downstream epitope. Rabbit Aff - Purified Hu IP
0.1 mg / €440.00
  Novus Biologicals Inc.

ALIX / PDCD6IP antibody

Western Blot: Alix Antibody [NBP1-49701]-Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells.  Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate.  ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

ALIX / PDCD6IP antibody

Immunohistochemistry-Paraffin: PDC6IP Antibody [NBP1-90201] - Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ALIX / PDCD6IP

ALIX / PDCD6IP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ALIX / PDCD6IP (1-392, His-tag)

ALIX / PDCD6IP Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

ALIX / PDCD6IP (1-392, His-tag)

ALIX / PDCD6IP Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

ALIX / PDCD6IP

ALIX / PDCD6IP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP

ALIX / PDCD6IP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP

ALIX / PDCD6IP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ALIX / PDCD6IP

ALIX / PDCD6IP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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