ALIX / PDCD6IP antibody

Principal name

ALIX / PDCD6IP antibody

Alternative names for ALIX / PDCD6IP antibody

PDCD6-interacting protein, ALG-2-interacting protein 1, Hp95, KIAA1375, Programmed cell death 6-interacting protein

SwissProt ID

Q8WUM4 (Human), Q9QZA2 (Rat), Q9WU78 (Mouse)

Gene ID

10015 (PDCD6IP), 18571 (Pdcd6ip), 501083

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Xen (Xenopus)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of ALIX / PDCD6IP antibody

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumel endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several altertively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012].

General readings

Morita E., et al. (2007) EMBO J. 26(19):4215-27.
Katoh K., et al. (2004) Arch Biochem Biophys. 421(1):159-65.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALIX / PDCD6IP antibody

  PDCD6IP mouse monoclonal antibody, clone OTI1A4 (formerly 1A4)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDCD6IP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507312) Mouse IgG2a OTI1A4 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALIX / PDCD6IP antibody

  Mouse Monoclonal anti-Alix Antibody  
NIH3T3 whole cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Alix antibody (clone 3A9). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluninescence detection. Mouse IgG1 3A9 Purified Hu, Ms ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALIX / PDCD6IP antibody

  Mouse Monoclonal anti-Alix Antibody  
ALIX / PDCD6IP Mouse 3A9 Purified Hu, Ms ICC/IF, IP, WB
25 µg / €248.00
  OriGene Technologies, Inc.

ALIX / PDCD6IP antibody

Western blot analysis of HeLa whole cell lysate probed with Mouse Anti-ALIX (AM01318PU-N) F(ab')2 Rabbit Anti-Mouse IgG:HRP (SP1012HRP). Mouse IgG1 3A9 Purified Hu, Ms, Rt, Xen E, ICC/IF, IP, P, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

ALIX / PDCD6IP antibody

Western Blot: ALIX antibody (8E8) staining of 10 µg (lane 1) and 100 µg (lane 2) of recombinant GST-ALIX Mouse IgG1 8E8 Purified Hu C, E, WB
0.1 mg / €400.00
  Acris Antibodies GmbH

ALIX / PDCD6IP antibody

BHK cell line transfected with Alix gene and stained with ALIX antibody Cat.-No AP54754SU-N Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

PDCD6IP - human gene knockout kit via CRISPR

PDCD6IP - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

PDCD6IP - human gene knockout kit via CRISPR

PDCD6IP - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDCD6IP - human gene knockout kit via CRISPR

PDCD6IP - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

PDCD6IP - human gene knockout kit via CRISPR

PDCD6IP - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdcd6ip - mouse gene knockout kit via CRISPR

Pdcd6ip - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Pdcd6ip - mouse gene knockout kit via CRISPR

Pdcd6ip - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdcd6ip - mouse gene knockout kit via CRISPR

Pdcd6ip - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Pdcd6ip - mouse gene knockout kit via CRISPR

Pdcd6ip - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ALIX / PDCD6IP

  Recombinant protein of human programmed cell death 6 interacting protein (PDCD6IP)  
ALIX / PDCD6IP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ALIX / PDCD6IP (1-392, His-tag)

ALIX / PDCD6IP Human Purified > 95 % by SDS-PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

ALIX / PDCD6IP (1-392, His-tag)

ALIX / PDCD6IP Human Purified > 95 % by SDS-PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

PDCD6IP overexpression lysate

PDCD6IP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, PDCD6IP (mGFP-tagged) - Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, PDCD6IP (mGFP-tagged) - Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, PDCD6IP (Myc-DDK tagged) - Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, PDCD6IP (Myc-DDK tagged) - Human programmed cell death 6 interacting protein (PDCD6IP), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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