Alkaline phosphatase / ALPI / IAP antibody

Principal name

Alkaline phosphatase / ALPI / IAP antibody

Alternative names for Alkaline phosphatase / ALPI / IAP antibody

Intestinal alkaline phosphatase

SwissProt ID

P09923 (Human), P15693 (Rat), P19111 (Bovin), P24822 (Mouse)

Gene ID

248 (ALPI), 280993

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Goat, Mouse, Sheep

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Flow Cytometry (F)

Background of Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

General readings

This product has been used in:Lai AP et al. Bone marrow macrophages and megakaryocytes express common acute lymphoblastic leukaemia antigen. Leuk Res 9:1155-9 (1985). PubMed PMID: 2933564

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alkaline phosphatase / ALPI / IAP antibody

Sample (30 ug of whole cell lysate). A: Hep G2 . 7.5% SDS PAGE. TA308737 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPI / IAP antibody

Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Rabbit Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPI / IAP antibody

Both the antiserum and IgG fractions of anti-Alkaline Phosphatase (Human Intestine) are shown to detect under reducing conditions of SDS-PAGE the 60,000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:5,000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] diluted 1:4,000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results. Rabbit Serum Hu E, WB
2 ml / €900.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPI / IAP antibody

Western blot analysis of lysate from human placenta tissue,using ALPI Antibody (Center)(Cat. #TA324568).TA324568 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane. Rabbit Ig Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPI / IAP antibody

Formalin-Fixed Paraffin-Embedded Human Small intestine stained with Alkaline Phosphatase Antibody Cat.-No AP07542PU-N at 5 µg/ml after heat-induced antigen retrieval. Rabbit Purified Hu E, ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Goat IgG HRP Bov C, E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Goat IgG Purified Bov Dot, E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Goat Serum Bov E, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Sheep IgG Azide Free Bov Dot, E, IP, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Sheep IgG HRP Bov E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Sheep IgG Purified Bov Dot, E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Rabbit IgG Aff - Purified Bov Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Small intestine, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Purified Hu P
50 µg / €470.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Goat Serum Bov C, E
1 ml / €340.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Rabbit Biotin Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Rabbit HRP Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Rabbit Serum Bov E, IP, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Rabbit Biotin Bov E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Alkaline phosphatase / ALPI / IAP Rabbit HRP Bov E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPI / IAP antibody

Western blot of rabbit anti Alkaline phosphatase (calf intestine) antibody Cat.-No. R1053P. Lane 1: Bovine Alkaline phosphatase. Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline phosphatase (calf intestine) antibody at 1/1,000 overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1/40,000 for 30 min at RT. Blocking buffer for 30 min at RT. Predicted/observed size: 57 kDa, 60 kDa for Alkaline phosphatase (calf intestine). Rabbit Purified Bov C, E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

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