Alkaline phosphatase / ALPL antibody

Principal name

Alkaline phosphatase / ALPL antibody

Alternative names for Alkaline phosphatase / ALPL antibody

AP-TNAP, TNSALP, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase liver/bone/kidney isozyme

SwissProt ID

P05186 (Human), P08289 (Rat), P09242 (Mouse), P09487 (Bovin), Q29486 (Felca), Q92058 (Chick)

Gene ID

249 (ALPL), 945041 (phoA), 25586 (Alpl)

Available reactivities

Fe (Feline), Hu (Human), Por (Porcine), Rb (Rabbit), Bov (Bovine), E. coli (Escherichia coli), Bac (Bacteria), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit

Available applications

Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of Alkaline phosphatase / ALPL antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

General readings

J Rheumatol. 2009 Dec;36(12):2758-65.
Calcif Tissue Int. 2009 Sep;85(3):228-34.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alkaline phosphatase / ALPL antibody

Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) (Cat.#TA324563). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene. Rabbit IgG Purified Hu, Ms F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPL antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ALPL rabbit polyclonal antibody. (TA590021) Rabbit IgG Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPL antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ALPL antibody Cat.-No AM06737PU-N with DAB staining. Mouse IgG1 2F4 Purified Hu E, F, P, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL antibody

Western blot analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. Mouse IgG 4H1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alkaline phosphatase / ALPL antibody

Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (EPR4477) [NBP1-95392] - Analysis of paraffin-embedded human kidney tissue using anti-ALPL. Rabbit EPR4477 Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Alkaline phosphatase / ALPL antibody

Sample (30 ug of whole cell lysate). A: Hela. 7.5% SDS PAGE. TA308847 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPL antibody

Alkaline phosphatase / ALPL Mouse IgG1 Purified Bov, Hu E, WB
50 µg / €345.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPL antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human tonsil tissue . Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using ALPL antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPL antibody

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ALPL antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / ALPL antibody

Alkaline phosphatase / ALPL Mouse IgG3 BGN/03/66KF44 Purified Hu E, P
0.2 mg / €390.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL (N-term) antibody

Western blot analysis of ALPL Antibody (N-term) (AP11472PU-N) in mouse brain tissue lysates (35 µg/lane). ALPL(arrow) was detected using the purified Pab (1:60 dilution). Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL (N-term) antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (small intestine), staining ALPL in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL antibody

Alkaline phosphatase / ALPL Rabbit Serum Rt C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL antibody

Alkaline phosphatase / ALPL Mouse IgG1 BGN/03/661 Purified Hu E, WB
0.2 mg / €540.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL antibody

Western Blot analysis of bacterial alkaline phosphatase tagged, HCA106 using Mouse anti Human alkaline phosphatase (BM217), followed by SP1012HRP. Mouse IgG1 BG/03/662 Purified Hu E, WB
0.2 mg / €390.00
  Acris Antibodies GmbH

Alkaline phosphatase / ALPL antibody

ALPL MaxPab polyclonal antibody. Western Blot analysis of ALPL expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Alkaline phosphatase / ALPL antibody

Immunoprecipitation of ALPL transfected lysate using anti-ALPL MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ALPL MaxPab mouse polyclonal antibody (B01) (H00000249-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

Alkaline phosphatase / ALPL antibody

Western Blot analysis of ALPL expression in transfected 293T cell line (H00000249-T01) by ALPL MaxPab polyclonal antibody.

Lane 1: ALPL transfected lysate(57.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Alkaline phosphatase / ALPL antibody

Alkaline phosphatase / ALPL Mouse IgG1 3A7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alkaline phosphatase / ALPL antibody

Western blot analysis of ALPL polyclonal antibody ( Cat # PAB3138 ) in mouse brain tissue lysates ( 35 µg/lane ) . ALPL ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

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