Alkaline phosphatase / PLAP / ALPP antibody

Principal name

Alkaline phosphatase / PLAP / ALPP antibody

Alternative names for Alkaline phosphatase / PLAP / ALPP antibody

PLAP-1, Alkaline phosphatase Regan isozyme, Alkaline phosphatase, placental type

SwissProt ID

P05187 (Human)

Gene ID

250 (ALPP)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), African clawed frog, Hst (Hamster), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Paraffin Sections (P), Frozen Sections (C), Enzyme Immunoassay (E), Flow Cytometry (F), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), ELISA (detection) (E(detection))

Background of Alkaline phosphatase / PLAP / ALPP antibody

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. ALPP is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3\' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

General readings

1. Fisken, J et al. (1989. Serum placental-like alkaline phosphatase (PLAP): a novel combined enzyme linked immunoassay for monitoring ovarian cancer. J. Clin. Pathol. 42:
40 - 45.
2. Tucker, D. F. et al. (1985). Serum marker potential of placental alkaline phosphate-like activity in testicular germ cell tumours evauated by H17E2 monoclonal antibody assay. Br. J. Cancer. 51: 631 - 639.
3. Epentos, A. A. et al. (1984). An immunohistological study of testicular germ cell tumours using different monoclonal antibodies against placental alkaline phophatase. Br. J. Cancer. 49: 11-15.
4. Kalofonos, H. P. et al. (1990). Immunolocalisation of testicular tumours using radiolabelled monoclonal antibody to placental alkalin phosphate. J. Nucl. Med. Allied. Sci. 34: 294 298.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alkaline phosphatase / PLAP / ALPP antibody

Formalin-Fixed, Paraffin-Embedded Human seminoma (4 µm) stained with anti-PLAP antibody (DB 047) shows strong positive membrane immunostaining of tumor cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG P16-D Aff - Purified Hu P
0.1 ml / €220.00
  DB Biotech, spol. s r.o.

Sample sizes available

Alkaline phosphatase / PLAP / ALPP antibody

Formalin-Fixed, Paraffin-Embedded Human seminoma (4 µm) stained with anti-PLAP antibody (DB 047) shows strong positive membrane immunostaining of tumor cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG P16-D Aff - Purified Hu P
0.2 ml / €290.00
  DB Biotech, spol. s r.o.

Sample sizes available

Alkaline phosphatase / PLAP / ALPP antibody

Formalin-Fixed, Paraffin-Embedded Human seminoma (4 µm) stained with anti-PLAP antibody (DB 047) shows strong positive membrane immunostaining of tumor cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG P16-D Aff - Purified Hu P
0.5 ml / €360.00
  DB Biotech, spol. s r.o.

Sample sizes available

Alkaline phosphatase / PLAP / ALPP antibody

Formalin-Fixed, Paraffin-Embedded Human seminoma (4 µm) stained with anti-PLAP antibody (DB 047) shows strong positive membrane immunostaining of tumor cells. Kindly performed and provided by Katarína Poliaková, MD and Ľubomír Straka, MD, Ph. D., from Clinical Pathology Prešov, Ltd., Prešov, Slovak republic. Rabbit IgG P16-D Aff - Purified Hu P
1 ml / €540.00
  DB Biotech, spol. s r.o.

Sample sizes available

Alkaline phosphatase / PLAP / ALPP antibody

AM11186PU PLAP antibody staining of Human seminoma. Rabbit SP15 Supernatant Hst, Hu, Ms, Rt P
0.5 ml / €390.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

AM11186PU PLAP antibody staining of Human seminoma. Rabbit SP15 Supernatant Hu, Ms, Rt P
1 ml / €550.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

AM11186PU PLAP antibody staining of Human seminoma. Rabbit SP15 Supernatant Hu, Ms, Rt P
0.1 ml / €290.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Sample (30 ug of whole cell lysate). A: H1299. B: Hela. C: Hep G2 . 7.5% SDS PAGE. TA308470 diluted at 1:500. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / PLAP / ALPP antibody

ALPP Antibody (N-term) (Cat. #TA324348) western blot analysis in mouse placenta tissue lysates (35ug/lane).This demonstrates the ALPP antibody detected the ALPP protein (arrow). Mouse IgG1 421CT8.1.1 Ascites Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / PLAP / ALPP antibody

Western Blot: Alkaline Phosphatase, Placental Antibody (8B6) [TA336510] - Analysis of Alkaline Phosphatase (Placental) expression in JAR whole cell lysate. Mouse IgG2a 8B6 Purified Hu C, E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / PLAP / ALPP antibody

Western blot detection of Placental alkaline phosphatase (PLAP) in Hela,Lncap,human Placenta and A549 cell lysates and using Placental alkaline phosphatase (PLAP) mouse mAb (1:500 diluted).Predicted band size: 76KDa.Observed band size:76KDa. Mouse IgG2b 8F10-2C10-C9 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alkaline phosphatase / PLAP / ALPP antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALPP (RC210504, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALPP. Mouse IgG2b OTI1H2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alkaline phosphatase / PLAP / ALPP antibody

Alkaline phosphatase / PLAP / ALPP Mouse IgG2a H7E8 Aff - Purified Hu C, E, P, WB
0.1 mg / €190.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer (A), stomach cancer (B) using ALPP antibody Cat.-No AM06465SU-N with DAB staining. Mouse IgG2b 3E5 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Formalin-Fixed Paraffin-Embedded Human Brain, cerebellum stained with ALPP Antibody Cat.-No AM08240PU-N (Clone H7E8) Mouse IgG2a H7E8 Aff - Purified Hu C, E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP (Internal) antibody

AM08281PU-N ALPP antibody staining of Formalin-Fixed, Paraffin-Embedded Human Placenta at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu P
0.125 ml / €470.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Alkaline phosphatase / PLAP / ALPP Mouse IgG1 MIG-P1 Supernatant Hu P
1.5 ml / €680.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Alkaline phosphatase / PLAP / ALPP Mouse IgG1 V17.1 Purified C, E
0.1 mg / €300.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Alkaline phosphatase / PLAP / ALPP Mouse IgG1 R92F6 Supernatant Hu C, P
1 ml / €620.00
  Acris Antibodies GmbH

Alkaline phosphatase / PLAP / ALPP antibody

Human Placenta (formalin-fixed, paraffin-embedded) stained with ALPP antibody AP22628PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Purified Hu E, ICC/IF, IP, P, WB
0.1 mg / €470.00
  Acris Antibodies GmbH

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