ALKBH1 antibody

Principal name

ALKBH1 antibody

Alternative names for ALKBH1 antibody

ALKBH, ABH, Alkylated DNA repair protein alkB homolog 1

SwissProt ID

Q13686 (Human)

Gene ID

8846 (ALKBH1), 388468 (POTEC)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of ALKBH1 antibody

This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of D alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. Dioxygese that repairs alkylated single-stranded D and R containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen; alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation. Has D lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded D and double-stranded D at abasic sites; with the greatest activity towards double-stranded D with two abasic sites.

General readings

"Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA." Westbye M.P., Feyzi E., Aas P.A., Vagbo C.B., Talstad V.A., Kavli B., Hagen L., Sundheim O., Akbari M., Liabakk N.B., Slupphaug G., Otterlei M., Krokan H.E. J. Biol. Chem. 283:25046-25056(2008)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALKBH1 antibody

Sample (30 ug of whole cell lysate). A: NT2D1. 10% SDS PAGE. TA308976 diluted at 1:1000. Rabbit IgG Aff - Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALKBH1 antibody

Predicted band size: 44 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALKBH1 antibody

Predicted band size: 44 kDa . Positive control: Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/250 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALKBH1 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ALKBH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805636) Mouse IgG1 OTI4C4 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALKBH1 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ALKBH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805682) Mouse IgG1 OTI1B8 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALKBH1 (N-term) antibody

Human Fetal Kidney; WB Suggested Anti-ALKBH1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Kidney; ALKBH1 antibody - N-terminal region (AP46320PU-N) in Human Fetal Kidney cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  Acris Antibodies GmbH

ALKBH1 antibody

ALKBH1 Mouse Purified Hu E(detection), ICC/IF, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

ALKBH1 antibody

Western Blot analysis of ALKBH1 expression in transfected 293T cell line (H00008846-T02) by ALKBH1 MaxPab polyclonal antibody.

Lane 1: ALKBH transfected lysate(42.79 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

ALKBH1 (N-term) antibody

Human Fetal Kidney; WB Suggested Anti-ALKBH1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Kidney; ALKBH1 antibody - N-terminal region (ARP61830_P050) in Human Fetal Kidney cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ALKBH1 antibody

ALKBH1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ALKBH1

ALKBH1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ALKBH1 293T Cell Transient Overexpression Lysate(Denatured)

ALKBH1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

ALKBH Lysate(Denatured)

ALKBH Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

13 Item(s)

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