ALOX5 / LOG5 antibody

Principal name

ALOX5 / LOG5 antibody

Alternative names for ALOX5 / LOG5 antibody

5-lipoxygenase, 5-LO, Arachidonate 5-lipoxygenase

SwissProt ID

P09917 (Human), P12527 (Rat), P48999 (Mouse)

Gene ID

240 (ALOX5)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Prim (Primate), Can (Canine), Bov (Bovine), Rb (Rabbit), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of ALOX5 / LOG5 antibody

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Drazen JM, Lilly CM, Sperling R, Rubin P, Israel E (1994) Role of cysteinyl leukotrienes in spontaneous asthmatic
responses. Adv. Prostaglandin Thromboxane Leukot Res 22:251-262.
2. Dwyer JH, Allayee H, Dwyer KM, Fan J, Wu H, Mar R, Lusis AJ, Mehrabian M (2004) Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis. New England J Med 350:29-37.
3. Funk, CD (2001) Prostaglandins and leukotrienes: advances in eicosanoid biology. Science 294:1871-1875.
4. Luo M, Jones SM, Phare SM, Coffey MJ, Peters-Golden M, Brock TG (2004) Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on Serine 523. J Biol Chem 279:41,512-41,520.
5. Wilborn J, Bailie M, Coffey M, Burdick M, Strieter R, Peters-Golden M. (1996) Constitutive activation of 5-lipoxygenase in the lungs of patients with idiopathic pulmonary fibrosis. J Clin Invest 97:1827-1836.

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)

Items 1 to 20 of 42 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ALOX5 / LOG5 pSer523 antibody

Figure 1. Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523  antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Rabbit IgG Aff - Purified Hu, Prim, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

ALOX5 / LOG5 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA807013)(1:150) Mouse IgG2a OTI3F1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALOX5 / LOG5 antibody

TA302414 (0.3µg/ml) staining of Rat Stomach lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ALOX5 / LOG5 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and thyroid cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue  using ALOX5 antibody at dilution 1/50, on the right is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ALOX5 antibody at dilution 1/50. (Original magnification: x 400) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALOX5 / LOG5 antibody

Predicted cell location: Cytoplasm . Positive control: Human esophagus and lung cancer tissue.Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue  using ALOX5 antibody at dilution 1/25, on the right is immunohistochemistry of paraffin-embedded Human lung cancer tissue using ALOX5 antibody at dilution 1/25. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALOX5 / LOG5 antibody

Western Blot: 5-Lipoxygenase Antibody [TA336527] - Detection of LOX5 in human peripheral leukocytes using TA336527 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2). Rabbit Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALOX5 / LOG5 antibody

Western Blot: 5-Lipoxygenase Antibody [TA336528] - Detection of LOX5 in human peripheral leukocytes using TA336528 at 0.5(Lane 1) and 2.0 ug/ml (Lane 2). Rabbit Purified Bov, Hu, Ms, Rb, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

ALOX5 / LOG5 antibody

Host: Rabbit; Target Name: ALOX5; Sample Tissue: HepG2 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

ALOX5 / LOG5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX5.(1:2000) Mouse IgG1 OTI2C5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALOX5 / LOG5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX5. Mouse IgG1 OTI2C7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALOX5 / LOG5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX5. Mouse IgG1 OTI2B7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALOX5 / LOG5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALOX5.(1:500) Mouse IgG1 OTI2D7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ALOX5 / LOG5 pSer523 antibody

Western blot of Rat hippocampal lysate showing phospho-specific immunolabelling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 Rabbit IgG Purified Hu, Mky, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

ALOX5 / LOG5 antibody

Immunohistochemistry analyzes of ALOX5 antibody (Cat.-No.: AP06493PU-N) in paraffin-embedded human skeletal muscle tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ALOX5 / LOG5 antibody

Human Colon, Adipocytes: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ALOX5 / LOG5 antibody

ALOX5 MaxPab rabbit polyclonal antibody. Western Blot analysis of ALOX5 expression in mouse spleen. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

ALOX5 / LOG5 antibody

ALOX5 polyclonal antibody ( Cat # PAB7097 ) ( 0.3 µg/ml ) staining of rat stomach lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ALOX5 / LOG5 antibody

Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of ALOX5 phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the ALOX5 (phospho S523) polyclonal antibody ( Cat # PAB9606 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Rabbit Hu, Rt WB
0.1 ml / €510.00
  Abnova Taiwan Corp.

ALOX5 / LOG5 antibody

Detection of ALOX5 in human peripheral leukocytes using ALOX5 polyclonal antibody ( Cat # PAB12488 ) at 0.5 ( Lane 1 ) and 2.0 µg/ml ( Lane 2 ). Rabbit Hu, Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

ALOX5 / LOG5 antibody

Detection of ALOX5 in human peripheral leukocytes using ALOX5 polyclonal antibody ( Cat # PAB12489 ) at 0.5 ( Lane 1 ) and 2.0 µg/ml ( Lane 2 ). Rabbit Bov, Hu, Ms, Rb, Rt WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

Items 1 to 20 of 42 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn