Alpha-1-antitrypsin antibody

Principal name

Alpha-1-antitrypsin antibody

Alternative names for Alpha-1-antitrypsin antibody

SERPINA1, AAT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase

SwissProt ID

A1ILJ0 (Canfa), P01009 (Human), P12725 (Sheep), P17475 (Rat), P34955 (Bovin), P50447 (Pig)

Gene ID

5265 (SERPINA1), 480422, 397688

Available reactivities

Hu (Human), Mam (Mammalia), Ms (Mouse), Rt (Rat), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat, Chicken, Sheep

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunodiffusion (ID), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Flow Cytometry (F), Luminex (LMNX)

Background of Alpha-1-antitrypsin antibody

The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. All eleven variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

General / background references:Mahadeva R et al. 6-mer peptide selectively anneals to a pathogenic serpin conformation and blocks polymerization. Implications for the prevention of Z alpha(1)-antitrypsin-related cirrhosis. J Biol Chem 277:6771-4 (2002). PubMed PMID: 11773044

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-1-antitrypsin antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500374) Mouse IgG1 OTI9A1 Purified Can, Hu, Mky F, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent WB. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used to detect target proteins under reducing conditions in albumin depleted human serum. Primary antibody: Each primary antibody at 1:1000 . Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000. Goat Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Goat-anti-Alpha-1-Anti-Trypsin (TA319139, red), Rabbit anti-Transferrin , and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing (R) conditions (+4% BME) in albumin depleted human serum with 320 ng of added GST. Membrane was probed with three primary antibodies at 1:1000 dilution. Detection shown was using DyLight549 Donkey anti-Rabbit IgG, DyLight 488 Donkey anti-Mouse IgG , and DyLight 649 Donkey anti-Goat IgG. Goat Serum Hu C, E, WB
2 ml / €900.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Alpha-1-antitrypsin Goat Biotin Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alpha-1-antitrypsin antibody

Alpha-1-antitrypsin Goat HRP Hu C, E, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alpha-1-antitrypsin antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500068) Mouse IgG2b OTI1C2 Purified Hu F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Alpha-1-antitrypsin Mouse IgG2b OTI3C5 Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500371) Mouse IgG1 OTI5B12 Purified Can, Hu, Mky IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500375) Mouse IgG1 OTI15H10 Purified Can, Hu, Mky IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500376) Mouse IgG1 OTI8E3 Purified Hu IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500378) Mouse IgG1 OTI11G2 Purified Can, Hu, Mky IP, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-1-antitrypsin antibody

Immunohistochemistry-Paraffin: alpha 1 Antitrypsin Antibody (9A1) [NBP1-47957] - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-SERPINA1mouse monoclonal antibody. (Dilution 1:50) Mouse IgG1 9A1 Aff - Purified Can, Hu, Mky F, IP, P, WB
0.1 ml / €530.00
  Novus Biologicals Inc.

Alpha-1-antitrypsin antibody

Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent WB. Lane 1: MW marker. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum. Primary antibody: Each primary antibody at 1:1000 .Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody . Rabbit Aff - Purified Hu E, P, WB
1 mg / €900.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Predicted cell location: Cytoplasm. Positive control: Human brain tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SERPINA1 antibody at dilution 1/45, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using SERPINA1 antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

TA326723 (0.3µg/ml) staining of Albumin-depleted Human Plasma lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin (230-241) antibody

TA326724 (0.1µg/ml) staining of Albumin-depleted Human Serum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Western blot analysis of extracts of various cell lines, using SERPINA1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Immunohistochemistry staining of Paraffin Tonsil tissue by A-1 Antitrypsin antibody (dilution: 1:500 - 1:2000; visualization of staining: Cytoplasmic) Rabbit Purified Hu P
1 ml / €200.00
  OriGene Technologies, Inc.

Alpha-1-antitrypsin antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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