Bov (Bovine), Chk (Chicken), Hu (Human), Rb (Rabbit), Ms (Mouse), Rt (Rat), Kangaroo, Trout, Xen (Xenopus), Por (Porcine), Ze (Zebrafish)
Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of alpha 1 cardiac muscle Actin / ACTC1 antibody
Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A type lamins and B type lamins. The A type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A type lamins coincides with cell differentiation and as A type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A type lamins.
Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11032-1-AP(ACTC1 antibody) at dilution of 1:500
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with ACTB/ACTC antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of ACTB/ACTC (arrow) using rabbit polyclonal ACTB/ACTC Antibody (Center) (AP11506PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACTB/ACTC gene (Lane 2) (Origene Technologies).
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (GTX101876) diluted at 1:200.Blue: Hoechst 33342 staining.
alpha Cardiac Muscle Actin antibody detects alpha Cardiac Muscle Actin protein at cytoplasm on mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse small intestine. alpha Cardiac Muscle Actin antibody (GTX101900) diluted at 1:500.
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.