Alpha-2-macroglobulin / A2M antibody

Principal name

Alpha-2-macroglobulin / A2M antibody

Alternative names for Alpha-2-macroglobulin / A2M antibody

Alpha-2-M, CPAMD5, Alpha 2 macroglobulin, PZP-like alpha-2-macroglobulin domain-containing

SwissProt ID

P01023 (Human), P06238 (Rat), Q7SIH1 (Bovin)

Gene ID

2 (A2M), 100911545 (LOC100911545), 513856

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Eq (Equine), Bov (Bovine)

Available hosts

Sheep, Chicken, Goat, Mouse, Rabbit

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P), Radioimmunoassay (R), Immunodiffusion (ID)

Background of Alpha-2-macroglobulin / A2M antibody

Alpha 2 macroglobulin is a serum protein which is mainly synthesised in the liver. It functions as a broad range irreversible proteinase inhibitor that forms a "trap" around most proteases. Alpha 2 macroglobulin is a tetrameric molecule, and with a molecular weight of 720 kDa, it so large a molecule that it tends to remain intravascular. Following conformational change the proteinase is deposited within a central cavity where it is still active but prevented from further contact with its substrate. Removal of the complex is mediated by the generation of recognition sites which react with receptors on a variety of cells including macrophages and liver cells. Alpha 2 macroglobulin is localized on the surface of peripheral blood lymphocytes.

General readings

General / background references:Dolmer K et al. Crystallisation and preliminary X-ray analysis of the receptor-binding domain of human and bovine alpha 2-macroglobulin. FEBS Lett 372:93-5 (1995). PubMed PMID: 7556651

Items 1 to 20 of 43 total

per page
Page:
  1. 1
  2. 2
  3. 3

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-2-macroglobulin / A2M antibody

Goat anti Alpha-2-Macroglobulin (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat. Goat Serum Hu E, IP, WB
2 ml / €900.00
  OriGene Technologies, Inc.

Alpha-2-macroglobulin / A2M antibody

Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms. Goat Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-2-macroglobulin / A2M antibody

Western blot analysis of extracts of HepG2 cell lines, using A2M antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-2-macroglobulin / A2M antibody

Anti-A2M antibody (0.5µg/ml) staining of Human Albumin-depleted Serum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Rabbit IgG Serum Bov E
1 ml / €340.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Liver. Goat IgG Purified Hu E, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M (C-term) antibody

Human Liver (formalin-fixed, paraffin-embedded) stained with A2M antibody AP22655PU-N at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Hu E, IP, P, WB
0.1 mg / €470.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Goat Serum Rt ID, IP
1 ml / €250.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M (C-term) antibody

A2M Antibody (C-term) (Cat. #AP50006PU-N) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the A2M antibody detected the A2M protein (arrow) Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M (N-term) antibody

A2M Antibody (N-term) (Cat. #AP50007PU-N) western blot analysis in mouse plasma lysates (35 g/lane).This demonstrates the A2M antibody detected the A2M protein (arrow) Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Mouse IgG1 2N1/10 Purified Hu E, R
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Sheep Ig Fraction Hu C, E
1 ml / €330.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Goat IgG Biotin Hu E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Goat IgG HRP Hu E, IP, P, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Alpha-2-macroglobulin / A2M antibody

A2M MaxPab polyclonal antibody. Western Blot analysis of A2M expression in human liver. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Alpha-2-macroglobulin / A2M antibody

Alpha-2-macroglobulin / A2M Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Alpha-2-macroglobulin / A2M antibody

A2M MaxPab rabbit polyclonal antibody. Western Blot analysis of A2M expression in human placenta. Rabbit Serum Hu E(detection), WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Alpha-2-macroglobulin / A2M antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between IL1B and A2M. HeLa cells were stained with anti-IL1B rabbit purified polyclonal 1:1200 and anti-A2M mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2B5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-2-macroglobulin / A2M antibody

Immunoblot analysis of human plasma protein. Lane 1 : Alpha 2-macroglobulin isolated from human plasma. Lane 2 : Human plasma. ( * N. S : Nonspecific band ) Mouse IgG1 2D9 Hu E, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Items 1 to 20 of 43 total

per page
Page:
  1. 1
  2. 2
  3. 3
  • LinkedIn