Alpha-actinin-1 / ACTN1 antibody

Principal name

Alpha-actinin-1 / ACTN1 antibody

Alternative names for Alpha-actinin-1 / ACTN1 antibody

Alpha actinin cytoskeletal isoform, Non-muscle alpha-actinin 1, F-actin cross-linking protein

SwissProt ID

B8JHU4, P05094 (Chick), P12814 (Human), Q3B7N2 (Bovin), Q7TPR4 (Mouse), Q9Z1P2 (Rat)

Gene ID

87 (ACTN1), 373918, 560400 (actn1)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Alpha-actinin-1 / ACTN1 antibody

Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Youssoufian, H.; McAfee, M.; Kwiatkowski, D. J. : Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am. J. Hum. Genet. 47: 62-72, 1990.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACTN1/2/3/4 antibody

Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ACTN1/2/3/4 antibody

Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ACTN1/2/3/4 antibody

Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt, Ze IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Alpha-actinin-1 / ACTN1 antibody

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072) Mouse IgG2a OTI7A4 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-actinin-1 / ACTN1 antibody

ACTN1 antibody - N-terminal region validated by WB using 1.-2.Rat cortical neurons ctr siRNA (30ug); 2. Rat cortical neurons ACTN1 siRNA (30ug) at 1:1000-1:2000. Rabbit IgG Purified Bov, Can, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

rACTNX-GFP; . Sample Type :. ACTNX-GFP transfected COS-7 cells. Primary Antibody Dilution :. 1:1000. Secondary Antibody :. Anti-rabbit-Alexa-Fluor 568. Secondary Antibody Dilution :. 1:100. Color/Signal Descriptions :. Green: GFP Red: ACTN1. Gene Name :. ACTN1. Submitted by :. Johannes W. Hell, Ph.D., Professor, Department of Pharmacology, University of California, Room 2419D Tupper Hall, Davis, CA 95616-5270, Phone: (530) 752 6540, Lab: (530) 752 7551, FAX: (530) 752 7710; ACTN1 antibody - N-terminal region (ARP58413_P050) in rACTNX-GFP cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Alpha-actinin-1 / ACTN1 antibody

Western blot analysis of extracts from K562 cells, using ACTN1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

Predicted cell location: Cytoplasm. Positive control: Human thyroid and colon cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ACTN1 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

Western blot analysis of extracts of various cell lines using ACTN1 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 (596-609) antibody

TA329085 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

Alpha-actinin-1 / ACTN1 Mouse IgG1 SA-20 Purified Hu, Ms, Rb, Rt ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

Alpha-actinin-1 / ACTN1 Mouse IgG1 CB11 Ig Fraction Chk, Hu C, E, WB
0.1 mg / €470.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : PC-3 cell lysate 
Lane 2. : Balb/3T3 cell lysate 
Lane 3. : A549 cell lysate Mouse IgG2a AT1D10 Purified Hu E, F, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : PC-3 cell lysate 
Lane 2. : Balb/3T3 cell lysate 
Lane 3. : A549 cell lysate Mouse IgG2a AT1D10 Purified Hu E, F, WB
50 µl / €220.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 (N-term) antibody

Western blot analysis of ACTN1 Antibody (N-term) (AP17086PU-N) in MDA-MB231 cell line lysates (35 µg/lane). ACTN1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

ACTN1 polyclonal antibody (A01), Lot # 051128JC01 Western Blot analysis of ACTN1 expression in MES-SA/Dx5 ( Cat # L021V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Alpha-actinin-1 / ACTN1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between ACTN1 and CAMK2A. HeLa cells were stained with anti-ACTN1 rabbit purified polyclonal 1:1200 and anti-CAMK2A mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Alpha-actinin-1 / ACTN1 antibody

Western Blot analysis of ACTN1 expression in transfected 293T cell line by ACTN1 monoclonal antibody (M01), clone 3F1.

Lane 1: ACTN1 transfected lysate(103.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG3 3F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-1 / ACTN1 antibody

Alpha-actinin-1 / ACTN1 Mouse IgG3 3F1 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-1 / ACTN1 antibody

Alpha-actinin-1 / ACTN1 Mouse IgG1 SA-20 Hu, Ms, Rb, Rt C, P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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