Alpha-actinin-1 / ACTN1 antibody

Principal name

Alpha-actinin-1 / ACTN1 antibody

Alternative names for Alpha-actinin-1 / ACTN1 antibody

Alpha actinin cytoskeletal isoform, Non-muscle alpha-actinin 1, F-actin cross-linking protein

SwissProt ID

B8JHU4, P05094 (Chick), P12814 (Human), Q3B7N2 (Bovin), Q7TPR4 (Mouse), Q9Z1P2 (Rat)

Gene ID

87 (ACTN1), 109711 (Actn1)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Chk (Chicken), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Frozen Sections (C), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Alpha-actinin-1 / ACTN1 antibody

Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Youssoufian, H.; McAfee, M.; Kwiatkowski, D. J. : Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene. Am. J. Hum. Genet. 47: 62-72, 1990.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-actinin-1 / ACTN1 antibody

  alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4)  
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500072) Mouse IgG2a OTI7A4 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Alpha-actinin-1 / ACTN1 antibody

  Rabbit Polyclonal Anti-ACTN1 Antibody - N-terminal region  
ACTN1 antibody - N-terminal region validated by WB using 1.-2.Rat cortical neurons ctr siRNA (30ug); 2. Rat cortical neurons ACTN1 siRNA (30ug) at 1:1000-1:2000. Rabbit IgG Purified Bov, Can, Hu, Ms, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

  Rabbit polyclonal anti-ACTN1 antibody  
Western blot analysis of extracts from K562 cells, using ACTN1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

  Anti-ACTN1 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human thyroid and colon cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using ACTN1 antibody at dilution 1/10, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

  Rabbit anti-ACTN1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines using ACTN1 antibody. Rabbit IgG Purified Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 (596-609) antibody

  ACTN1 (aa596-609) Goat Polyclonal Antibody  
TA329085 (0.3µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-1 / ACTN1 antibody

Alpha-actinin-1 / ACTN1 Mouse IgG1 SA-20 Purified Hu, Ms, Rb, Rt ICC/IF, IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

Alpha-actinin-1 / ACTN1 Mouse IgG1 CB11 Ig Fraction Chk, Hu C, E, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : PC-3 cell lysate 
Lane 2. : Balb/3T3 cell lysate 
Lane 3. : A549 cell lysate Mouse IgG2a AT1D10 Purified Hu E, F, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTN1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : PC-3 cell lysate 
Lane 2. : Balb/3T3 cell lysate 
Lane 3. : A549 cell lysate Mouse IgG2a AT1D10 Purified Hu E, F, WB
50 µl / €220.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 (N-term) antibody

Western blot analysis of ACTN1 Antibody (N-term) (AP17086PU-N) in MDA-MB231 cell line lysates (35 µg/lane). ACTN1 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-actinin-1 / ACTN1 antibody

Western blot analysis of extracts from 293/HeLa/A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

Alpha-actinin-1 / ACTN1 antibody

Western blot analysis of extracts from 293/HeLa/A549 cells, using ACTN1/2/3/4 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

ACTN1 - human gene knockout kit via CRISPR

ACTN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ACTN1 - human gene knockout kit via CRISPR

ACTN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ACTN1 - human gene knockout kit via CRISPR

ACTN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ACTN1 - human gene knockout kit via CRISPR

ACTN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Actn1 - mouse gene knockout kit via CRISPR

Actn1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Actn1 - mouse gene knockout kit via CRISPR

Actn1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Actn1 - mouse gene knockout kit via CRISPR

Actn1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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