Alpha-actinin-2 / ACTN2 antibody

Principal name

Alpha-actinin-2 / ACTN2 antibody

Alternative names for Alpha-actinin-2 / ACTN2 antibody

Alpha actinin skeletal muscle isoform 2, F-actin cross-linking protein

SwissProt ID

P20111 (Chick), P35609 (Human), Q2YDR5, Q3ZC55 (Bovin), Q9JI91 (Mouse)

Gene ID

88 (ACTN2), 11472 (Actn2), 3641519

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Fe (Feline), Fi (Fish), Gt (Goat), Hst (Hamster), Por (Porcine), Rb (Rabbit), Sh (Sheep), Snake, Xen (Xenopus), L (Lizard), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of Alpha-actinin-2 / ACTN2 antibody

The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in posttal skeletal muscle, suggesting independent functiolpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and alogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-39 DC378038.1 17-55 40-2961 BC051770.1 1-2922 2962-3458 CB153006.1 65-561 3459-3637 AL359185.25 28531-28709 3638-4210 M86406.1 3609-4181 4211-4528 AL359185.25 29283-29600

General readings

1. Verhaart IE  et al. The Dynamics of Compound, Transcript, and Protein Effects After Treatment With 2OMePS Antisense Oligonucleotides in mdx Mice. Mol Ther Nucleic Acids 3:e148 (2014). WB .
2. Giudice J  et al. Alternative splicing regulates vesicular trafficking genes in cardiomyocytes during postnatal heart development. Nat Commun 5:3603 (2014). WB ; Mouse .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ACTN2 antibody

Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ACTN2 antibody

Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ACTN2 antibody

Immunohistochemical analysis of ACTN2 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Alpha-actinin-2 / ACTN2 (11-347) antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Alpha-actinin-2 / ACTN2 (215-500) antibody

Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Alpha-actinin-2 / ACTN2 antibody

Western blot analysis of extracts from HeLa cells, HepG2 cells and HUVEC cells, using Actin a-2/3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-2 / ACTN2 antibody

WB Suggested Anti-ACTN2 Antibody Titration: 0.625 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Muscle Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-2 / ACTN2 antibody

WB Suggested Anti-ACTN2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-2 / ACTN2 antibody

WB Suggested Anti-ACTN2 Antibody Titration: 1.25 ug/ml; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-2 / ACTN2 antibody

Western blot analysis of Actinin a 2/3 expression in HeLa cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-2 / ACTN2 (C-term) antibody

ACTN2 Antibody (C-term) (Cat. #AP50071PU-N) western blot analysis in mouse heart tissue lysates (35µg/lane).This demonstrates the ACTN2 antibody detected the ACTN2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-actinin-2 / ACTN2 antibody

Alpha-actinin-2 / ACTN2 Mouse IgG1 EA-53 Supernatant Hu C, P
1 ml / €470.00
  Acris Antibodies GmbH

Alpha-actinin-2 / ACTN2 antibody

Alpha-actinin-2 / ACTN2 Mouse IgG1 EA-53 Supernatant Hu C, P
0.5 ml / €310.00
  Acris Antibodies GmbH

Alpha-actinin-2 / ACTN2 antibody

Alpha-actinin-2 / ACTN2 Mouse Hu WB
50 µl / €370.00
  Abnova Taiwan Corp.

Alpha-actinin-2 / ACTN2 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actinin alpha-2/3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

Alpha-actinin-2 / ACTN2 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Actinin alpha-2/3 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

Alpha-actinin-2 / ACTN2 antibody

Human Muscle; WB Suggested Anti-ACTN2 Antibody Titration: 0.625ug/ml. ELISA Titer: 1:312500. Positive Control: Human Muscle; ACTN2 antibody - C-terminal region (ARP37422_T100) in Human Muscle cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

Alpha-actinin-2 / ACTN2 antibody

Human Muscle; Rabbit Anti-ACTN2 Antibody. Catalog Number: ARP41463. Paraffin Embedded Tissue: Human Muscle. Cellular Data: Skeletal muscle cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; ACTN2 antibody - C-terminal region (ARP41463_T100) in Human Muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

Alpha-actinin-2 / ACTN2 antibody

Alpha-actinin-2 / ACTN2 Mouse IgG1 SA-20 Ascites Hu, Ms, Rt C, P, WB
0.1 mg / €290.00
  Boster Immunoleader

Alpha-actinin-2 / ACTN2 antibody

Alpha-actinin-2 / ACTN2 Mouse IgM NH3 Aff - Purified Hu C, E, F, WB
0.25 mg / €360.00
  Novus Biologicals Inc.

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