Alpha-actinin-3 / ACTN3 antibody

Principal name

Alpha-actinin-3 / ACTN3 antibody

Alternative names for Alpha-actinin-3 / ACTN3 antibody

Alpha actinin skeletal muscle isoform 3, F-actin cross-linking protein

SwissProt ID

O88990 (Mouse), Q08043 (Human), Q0III9 (Bovin)

Gene ID

89 (ACTN3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Alpha-actinin-3 / ACTN3 antibody

The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

General readings

1. Moncman CL  et al. Development transitions of thin filament proteins in rat extraocular muscles. Exp Cell Res : (2012). WB, IHC-Fr ; Rat .

13 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Alpha-actinin-3 / ACTN3 antibody

Western blot analysis of extracts from HT-29 cells, using ACTN3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-3 / ACTN3 antibody

Predicted band size: 103 kDa . Positive control: Human fetal muscle tissue and hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Human fetal muscle tissue lysate Lane2: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-3 / ACTN3 antibody

Predicted band size: 103 kDa . Positive control: Hela and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/300 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Alpha-actinin-3 / ACTN3 antibody

Western blot (WB) analysis of Actinin-α3 antibody (Cat.-No.: AP06785PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2:Mouse muscle tissue lysate Lane3:Rat muscel tissue lysate Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Alpha-actinin-3 / ACTN3 (N-term) antibody

Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Alpha-actinin-3 / ACTN3 (Center) antibody

ACTN3 Antibody (Center) (Cat. #AP50072PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTN3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Alpha-actinin-3 / ACTN3 antibody

Western blot analysis of extracts from HT-29 cells, using ACTN3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Alpha-actinin-3 / ACTN3 antibody

Western blot analysis of extracts from HT-29 cells, using ACTN3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Alpha-actinin-3 / ACTN3 antibody

Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Alpha-actinin-3 / ACTN3 antibody

Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt ICC/IF, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Alpha-actinin-3 / ACTN3 antibody

Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Bov, Hu, Ms, Rb, Rt ICC/IF, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Alpha-actinin-3 / ACTN3 antibody

Alpha-actinin-3 / ACTN3 Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Alpha-actinin-3 / ACTN3 antibody

Western Blot: Alpha Actinin 3 Antibody [NBP1-55292] - This Anti-ACTN3 antibody was used in Western Blot of Fetal Muscle tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

13 Item(s)

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